Characterization of Bifidobacterium asteroides Isolates

Pino, Alessandra; Benkaddour, Bachir; Inturri, Rosanna; Amico, Pietro; Vaccaro, Susanna; Russo, Nunziatina; Vaccalluzzo, Amanda; Agolino, Gianluigi; Caggia, Cinzia; Miloud, Hadadji; Randazzo, Cinzia Lucia

doi:10.3390/microorganisms10030655

Cited by 9 publications

(7 citation statements)

References 74 publications

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“…The function of probiotics to modulate intestinal microbiota and intestinal disease has been studied [ 59 ]. Due to their specific adaptations, probiotics can compete with other microorganisms, leading to alteration and modulation of the gut microbiota [ 60 ]. Moreover, probiotics can replace, adhere to and colonize with intestinal epithelial cells [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

Bifidobacterium animalis MSMC83 Improves Oxidative Stress and Gut Microbiota in D-Galactose-Induced Rats

et al. 2022

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The development of many chronic diseases is associated with an excess of free radicals leading to harmful oxidative stress. Certain probiotic strains have been shown to have antioxidant and anti-aging properties and are an important resource for development of microbial antioxidants. The present study aimed to explore the protection offered by Bifidobacterium animalis strain MSMC83 in a model of oxidative stress induced by D-galactose (D-gal). Male Sprague Dawley rats were randomly allocated to four groups: a control group injected with saline, a group injected subcutaneously with D-galactose, a probiotic group injected with D-galactose and administered B. animalis MSMC83 (109 CFU/mL) via daily oral gavage, and an ascorbic acid group. The probiotics significantly increased the superoxide dismutase, catalase, and glutathione peroxidase and significantly decreased the malondialdehyde in the plasma and livers of D-galactose-treated rats. Moreover, tumor necrosis factor-alpha level in the liver was significantly decreased. Furthermore, the treatment with B. animalis MSMC83 restored the microbiota diversity after D-galactose injection. Therefore, our results supported a beneficial role of B. animalis MSMC83 in alleviating oxidative stress through the increased expression of antioxidant enzymes and reduction of pro-inflammatory cytokines in rats. Our study suggests that B. animalis MSMC83 may be part of a healthy diet to prevent oxidative stress-associated diseases.

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Section: Discussionmentioning

confidence: 99%

Bifidobacterium animalis MSMC83 Improves Oxidative Stress and Gut Microbiota in D-Galactose-Induced Rats

et al. 2022

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“…Similarly, 98 putative virulence genes of 15 strains with whole genome analysis were detected in a study examining bifidobacteria causing bacteremia. 66 On the other hand, Pino et al 67 reported the absence of asa1, gelE, cylA, esp, and hyl genes for Bifidobacterium asteroides BA15 and BA17 strains. It is not possible to make a detailed comparison since there are limited studies on the virulence genes of Bifidobacterium spp.…”

Section: Hemolytic and Dnase Activitymentioning

confidence: 99%

Isolation, identification, and in vitro probiotic characterization of forty novel Bifidobacterium strains from neonatal feces in Erzurum province, Türkiye

Güler,

Çetin,

Albayrak

et al. 2024

J Sci Food Agric

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BACKGROUNDNeonatal feces are one of the most important sources for probiotic isolation. The purpose of this study was the isolation and identification of Bifidobacterium spp. from neonatal feces and the evaluation of in vitro probiotic properties of strains including safety tests.RESULTSA total of 40 isolates were obtained from 14 healthy newborns' feces in Erzurum province, Türkiye. By their rep‐PCR patterns and 16S rRNA gene sequences, isolates were identified as 26 Bifidobacterium breve and 14 Bifidobacterium longum. Fifteen of the isolates tolerated bile salts and showed high resistance to simulated gastric juice. Isolates exhibited varying rates of auto‐aggregation and hydrophobicity. In addition, most of the isolates displayed antibacterial activity against Escherichia coli O157:H7, Staphylococcus aureus ATCC 29213, Salmonella Typhimurium RSHMB 95091, and Pseudomonas aeruginosa ATCC 9027. However, only one strain showed bile salt hydrolase activity and two strains showed the ability to produce H2O2. Bifidobacterium strains were generally sensitive to the tested antibiotics and lacked kanamycin, gentamicin, and streptomycin resistance genes, and hemolytic and DNAse activities. On the other hand, it was determined that five strains had various virulence genes including gelE, esp, efaAfs, hyl, and ace.CONCLUSIONResults of the present study suggested that B. longum BH28, B. breve BH4 and B. breve BH5 strains have the potential as probiotic candidates for further studies. © 2024 Society of Chemical Industry.

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“…Bifidobacterium : Bifidobacterium is another commensal probiotic bacterium that participates in the homeostasis of the gut microorganism community ( 86 ). Bifidobacterium , the dominant species in the large intestine, requires an anaerobic environment ( 78 ).…”

Section: Culturing Ios With Microorganisms To Study Host-microbiome I...mentioning

confidence: 99%

Intestinal organoids as advanced modeling platforms to study the role of host-microbiome interaction in homeostasis and disease

Ahn¹,

Kang²,

Seo³

et al. 2022

BMB Rep

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After birth, animals are colonized by a diverse community of microorganisms. The digestive tract is known to contain the largest number of microbiome in the body. With emergence of the gut-brain axis, the importance of gut microbiome and its metabolites in host health has been extensively studied in recent years. The establishment of organoid culture systems has contributed to studying intestinal pathophysiology by replacing current limited models. Owing to their architectural and functional complexity similar to a real organ, co-culture of intestinal organoids with gut microbiome can provide mechanistic insights into the detrimental role of pathobiont and the homeostatic function of commensal symbiont. Here organoid-based bacterial co-culture techniques for modeling host-microbe interactions are reviewed. This review also summarizes representative studies that explore impact of enteric microorganisms on intestinal organoids to provide a better understanding of host-microbe interaction in the context of homeostasis and disease.

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Characterization of Bifidobacterium asteroides Isolates

Cited by 9 publications

References 74 publications

Bifidobacterium animalis MSMC83 Improves Oxidative Stress and Gut Microbiota in D-Galactose-Induced Rats

Bifidobacterium animalis MSMC83 Improves Oxidative Stress and Gut Microbiota in D-Galactose-Induced Rats

Isolation, identification, and in vitro probiotic characterization of forty novel Bifidobacterium strains from neonatal feces in Erzurum province, Türkiye

Intestinal organoids as advanced modeling platforms to study the role of host-microbiome interaction in homeostasis and disease

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