Characterization of bio‐fabricated silver nanoparticles for distinct anti‐fungal activity against sugarcane phytopathogens

Abstract

Advanced research, development, and application of silver nanoparticles is proceeding in recent times due to their incredible utilization in various fields. Present study was focused on the production, characterization, and antifungal activities of silver nanoparticles (AgNPs). An environment friendly extracellular biosynthetic approach was adopted to produce the AgNPs by using bacteria, fungi, and sugarcane husk. Agents used for reduction of silver to nanoparticles were taken from culture filtrate of plant growth promoting bacteria, Fusarium oxysporum and supernatant extract of sugarcane husk. Nanoparticles were also characterized by scanning electron microscopy (SEM). Synthesis of colloidal AgNPs was observed by UV–Visible diffused reflectance spectroscopy (UV–Vis DRS). Primary peak of surface plasmon resonance band was noticed around 339.782, 336.735, and 338.258 nm for bacterial, fungal, and sugarcane husk produced AgNPs. Structure of all biologically produced nanoparticles were crystalline cubic with nano size of 45.41, 49.06, and 42.75 nm for bacterial, fungal, and sugarcane husk‐based nanoparticles, respectively as calculated by Debye–Scherrer equation using XRD. Fourier transform infrared spectroscopy (FTIR) analysis revealed the presence of various compounds that aid in the reduction, capping, and stability of AgNPs. The antifungal activity of AgNPs was also investigated for sugarcane fungal pathogens Colletotricum falcatum and Fusarium moniliforme. All nanoparticles exhibit prominent antifungal activities. Maximum zone of fungal inhibition was noticed about 18, 19, and 21 mm for C. falcatum while 21, 20, and 24 mm for F. moniliforme in case of bacterial, fungal, and plant‐based nanoparticles (15 ppm), respectively. Best fungal inhibition was observed under application of sugarcane husk based AgNPs. Moreover, biologically produced AgNPs responded better towards the suppression of F. moniliforme in comparison to C. falcatum. Mentioned sources in present study can be ecofriendly nano‐factories for biosynthesis of AgNPs and mankind should benefit from their commercial application.

Development of topical silver nano gel formulation of Bixin: Characterization, and evaluation of anticancer activity

“…The IR spectral analysis of BOAgNPs with Carbapol 940 confirmed the occurrence of active groups such as O–H (stretching at 3341 cm −1 ), C = C (stretching at 1636 cm −1 ), C–H (stretching at 1451 cm −1 ) and C-O (stretching at 1230 cm −1 ) ( Amna et al, 2021 ). The observations suggested that mixing BOAgNPs with polymer (Carbapol 940) did not produce any chemical change.…”

“…The SEM analysis in the current study revealed that the particle size is a spherical shape. Such types of particles according to previous studies give a smooth texture to the formulation ( Amna et al, 2021 ). Besides, the particle size in the range of 50 – 110 µm was reported to be ideal for a drug molecule to achieve its intended therapeutic activity ( Das et al, 2022 ).…”

“…C–H and C = C. Further, stretching perceived at 1112 cm −1 , suggested the occurrence of C-O in the compound. And, bending vibrations observed at 1015 cm −1 are reported to be due to the C–H functional group in the test compound of the formulation ( Amna et al, 2021 ). Studies also suggested that the absence of any peak between 3000 – 2000 cm −1 suggested that functional groups might have played a role of reducing agents in converting Ag + to AgO ( Mughees and Wajid, 2021 ).…”

“…Furthermore, the pH of different formulations was found to be around 6.66. This value according to the literature is optimum for a formulation to avoid incidences of irritation when the drug is administered ( Amna et al, 2021 ). Further, the average drug amount in the formulation was estimated to be 83.9 %.…”

Nanomaterials as Nanofertilizers and Nanopesticides: An Overview

“…There is a lot of documents on the antifungal activity of AgNPs. In the most recent successes, in 2021, Amna et al [116] proved the prominent role of AgNPs against Colletotrichum falcatum and Fusarium moniliforme, fungal pathogens of sugarcane. Also, Al-Otibi et al [117] exhibited reduced growth mycelia of several plant pathogenic fungi, Helminthosporium rostratum, Fusarium solani, Fusarium oxysporum, and Alternaria alternata, exposed to bio-fabricated AgNPs using Cheeseweed mallow.…”