Characterization of biomass produced by Candida tropicalis ASY2 grown using sago processing wastewater for bioenergy applications and its fuel properties

Thangavelu, Kiruthika; Sundararaju, Pugalendhi; Srinivasan, Naganandhini; Uthandi, Sivakumar

doi:10.1007/s13399-020-01129-7

Cited by 7 publications

(4 citation statements)

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“…Applications of SWW include biogas [ 22 , 23 ], hydrogen [ 24 , 25 ], microbial lipid and biodiesel production using oleaginous yeast and fungi [ 21 , 26 – 29 ]. Several oleaginous fungi and yeasts were isolated previously from this wastewater for biodiesel production with simultaneous removal of pollutants [ 27 – 31 ].…”

Section: Introductionmentioning

confidence: 99%

Lovastatin production by an oleaginous fungus, Aspergillus terreus KPR12 using sago processing wastewater (SWW)

2022

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Background Lovastatin is one of the first statins to be extensively used for its cholesterol-lowering ability. It is commercially produced by fermentation. Species belonging to the genus Aspergillus are well-studied fungi that have been widely used for lovastatin production. In the present study, we produced lovastatin from sago processing wastewater (SWW) under submerged fermentation using oleaginous fungal strains, A. terreus KPR12 and A. caespitosus ASEF14. Results The intra- and extracellular concentrations of lovastatin produced by A. terreus KPR12 and A. caespitosus ASEF14 were lactonized. Because A. caespitosus ASEF14 produced a negligible amount of lovastatin, further kinetics of lovastatin production in SWW was studied using the KPR12 strain for 9 days. Lovastatin concentrations in the intra- and extracellular fractions of the A. terreus KPR12 cultured in a synthetic medium (SM) were 117.93 and 883.28 mg L–1, respectively. However, these concentrations in SWW were 142.23 and 429.98 mg L–1, respectively. The yeast growth inhibition bioassay confirmed the antifungal property of fungal extracts. A. terreus KPR12 showed a higher inhibition zone of 14 mm than the ASEF14 strain. The two-way analysis of variance (ANOVA; p < 0.01) showed significant differences in the localization pattern, fungal strains, growth medium, and their respective interactions. The lovastatin yield coefficient values were 0.153 g g–1 on biomass (YLOV/X) and 0.043 g g–1 on the substrate, starch (YLOV/S). The pollutant level of treated SWW exhibited a reduction in total solids (TS, 59%), total dissolved solids (TDS, 68%), biological oxygen demand (BOD, 79.5%), chemical oxygen demand (COD, 57.1%), phosphate (88%), cyanide (65.4%), and void of nutrients such as nitrate (100%), and ammonia (100%). Conclusion The starch-rich wastewater serves as a suitable medium for A. terreus KPR12 for the production of lovastatin. It simultaneously decontaminates the sago processing wastewater, enabling its reuse for irrigation/recreation. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Lovastatin production by an oleaginous fungus, Aspergillus terreus KPR12 using sago processing wastewater (SWW)

2022

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“…Its cell count was measured by flow cytometry (BD Biosciences, USA). Then, the wet yeast biomass was dried in a hot air oven at 40 °C until the constant weight reached [ 18 , 51 ]. The same procedure was followed to reproduce the experimental run.…”

Section: Methodsmentioning

confidence: 99%

“…In the direct transesterification process, the moisture content in the yeast biomass affects the overall biodiesel yield [ 56 , 57 ]. Hence, the moisture content of yeast biomass was determined according to the ASTM E871 method using a hot air oven at a temperature of 105 °C for 24 h [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

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Bioconversion of sago processing wastewater into biodiesel: Optimization of lipid production by an oleaginous yeast, Candida tropicalis ASY2 and its transesterification process using response surface methodology

et al. 2021

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Background Biodiesel is an eco-friendly and renewable energy source and a valuable substitute for petro-diesel. Sago processing wastewater (SWW), a by-product of the cassava processing industry, has starch content ranging from 4 to 7 g L–1 and serves as an outstanding source for producing microbial lipids by the oleaginous microorganisms. In the present study, Candida tropicalis ASY2 was employed to optimize single-cell oil (SCO) production using SWW and subsequent transesterification by response surface methodology. Variables such as starch content, yeast extract, airflow rate, pH, and temperature significantly influenced lipid production in a preliminary study. The lipid production was scaled up to 5 L capacity airlift bioreactor and its optimization was done by response surface methodology. The dried yeast biomass obtained under optimized conditions from 5 L bioreactor was subjected to a direct transesterification process. Biomass: methanol ratio, catalyst concentration, and time were the variables used to attain higher FAME yield in the transesterification optimization process. Results Under optimized conditions, the highest lipid yield of 2.68 g L–1 was obtained with 15.33 g L–1 of starch content, 0.5 g L–1 of yeast extract, and 5.992 L min–1 of airflow rate in a bioreactor. The optimized direct transesterification process yielded a higher FAME yield of 86.56% at 1:20 biomass: methanol ratio, 0.4 M catalyst concentration, and a time of 6.85 h. Conclusions Thus, this optimized process rendered the microbial lipids derived from C. tropicalis ASY2 as potentially alternative oil substitutes for sustainable biodiesel production to meet the rising energy demands.

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Forestry Biomass as Carbon Neutral Source for the Production of Biofuels and Aromatics

Tyagi

Anand

Jain

et al. 2023

Clean Energy Production Technologies

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Characterization of biomass produced by Candida tropicalis ASY2 grown using sago processing wastewater for bioenergy applications and its fuel properties

Cited by 7 publications

References 73 publications

Lovastatin production by an oleaginous fungus, Aspergillus terreus KPR12 using sago processing wastewater (SWW)

Lovastatin production by an oleaginous fungus, Aspergillus terreus KPR12 using sago processing wastewater (SWW)

Bioconversion of sago processing wastewater into biodiesel: Optimization of lipid production by an oleaginous yeast, Candida tropicalis ASY2 and its transesterification process using response surface methodology

Forestry Biomass as Carbon Neutral Source for the Production of Biofuels and Aromatics

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