Characterization of biomaterials polar interactions in physiological conditions using liquid–liquid contact angle measurements

Velzenberger, Elodie; Kirat, Karim El; Legeay, G.; Nagel, Marie‐Danielle; Pezron, Isabelle

doi:10.1016/j.colsurfb.2008.10.022

Cited by 33 publications

(24 citation statements)

References 39 publications

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“…The significance of this idea has been recognized for many years in the context of embryogenesis, where these forces are considered important to tissue ordering and morphogenetic movements (Beysens et al, 2000;Chen and Brodland, 2008;Foty et al, 1996). It has also been recognized in the context of biomaterials, where surface properties affect cell-implant interactions (Alves et al, 2007;Bren et al, 2004;Velzenberger et al, 2009). More recently, cell motions relevant to cancer metastases and tissue engineering have been explained in terms of cell surface tensions (Foty and Steinberg, 2004;Muller et al, 2007;Napolitano et al, 2007;Preetha et al, 2005;Siniscalco et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

“…Recent experimental and theoretical investigations suggest that cell surface properties govern cell motions and tissue selforganization in settings that range from embryology to cancer to regenerative medicine (Alves et al, 2007;Brodland, 2004;Foty et al, 1996;Foty and Steinberg, 2004;Lecuit and Lenne, 2007;Liu et al, 2008;Muller et al, 2007;Napolitano et al, 2007;Preetha et al, 2005;Siniscalco et al, 2008;Steinberg, 2007;Velzenberger et al, 2009). As reviewed in Brodland (2004) and elsewhere, surfaceassociated proteins and other subcellular structural components give rise to net tensions along cell-cell and cell-medium interfaces.…”

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confidence: 99%

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Cellular interfacial and surface tensions determined from aggregate compression tests using a finite element model

2009

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Cellular interfacial and surface tensions determined from aggregate compression tests using a finite element model

2009

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“…The effect of integrin-blocking peptide GRGDSP on HUES7 cell attachment to fibronectin (white bars, 10 mg ml -1 ) and its fragments: 120 kDa (light grey bars, 10 mg ml 21 ) and 70 kDa (dark grey bars, 160 mg ml 21 ). Control peptide GRGESP was used at a concentration of 1 mg ml 21 . Error bars are standard errors from the mean, where n ¼ 3.…”

Section: Discussionmentioning

confidence: 99%

“…Protein solution was aspirated and samples were washed three times with PBS without drying. Quantification of adsorbed TMR-Fn was carried out using a previously published procedure with a few modifications [21]. Briefly, adsorbed TMR-Fn was digested using 400 ml 2.5 per cent porcine trypsin solution made in 0.9 per cent sodium chloride solution (Sigma Aldrich, UK) for 24 h at 378C.…”

Section: Fibronectin Adsorption and Quantificationmentioning

confidence: 99%

Characterization of the interface between adsorbed fibronectin and human embryonic stem cells

Kalaskar

Downes

Murray

et al. 2013

J. R. Soc. Interface.

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The cell-substrate interface plays a key role in the regulation of cell behaviour. Defining the properties of this interface is particularly important for human embryonic stem (hES) cell culture, because changes in this environment can regulate hES cell differentiation. It has been established that fibronectincoated surfaces can promote the attachment, growth and maintenance of the undifferentiated phenotype of hES cells. We investigated the influence of the surface density of adsorbed fibronectin on hES cell behaviour in defined serum-free culture conditions and demonstrated that only 25 per cent surface saturation was required to maintain attachment, growth and maintenance of the undifferentiated phenotype. The influence of surface-adsorbed fibronectin fragments was compared with whole fibronectin, and it was demonstrated that the 120 kDa fragment central binding domain alone was able to sustain hES cells in an undifferentiated phenotype in a similar fashion to fibronectin. Furthermore, hES cell attachment to both fibronectin and the 120 kDa fragment was mediated by integrin a5b1. However, although a substrateattached synthetic arginine-glycine-aspartic acid (RGD) peptide alone was able to promote the attachment and spreading of fibroblasts, it was inactive for hES cells, indicating that stem cells have different requirements in order to attach and spread on the central fibronectin RGD-cell-binding domain. This study provides further information on the characteristics of the cellsubstrate interface required to control hES cell behaviour in clearly defined serum-free conditions, which are needed for the development of therapeutic applications of hES cells.

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“…However, recently there has been increasing evidence that the biological response is also affected by the physical properties of the material (4). In particular, it has been demonstrated that cells are influenced by the substrate topography (5, 6), rigidity (7, 8), anisotropy (9, 10), surface charge (11, 12), and wettability (13,14). From this perspective, cellular response to external stimuli goes far beyond the bare ability of the cell to chemically sense specific ECM ligands and includes a wide range of physical cues that are generated at, or act on, the interface between cells and the surrounding environment.…”

mentioning

confidence: 99%

Neurons sense nanoscale roughness with nanometer sensitivity

Brunetti

Maiorano

Rizzello

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

231

213

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The interaction between cells and nanostructured materials is attracting increasing interest, because of the possibility to open up novel concepts for the design of smart nanobiomaterials with active biological functionalities. In this frame we investigated the response of human neuroblastoma cell line (SH-SY5Y) to gold surfaces with different levels of nanoroughness. To achieve a precise control of the nanoroughness with nanometer resolution, we exploited a wet chemistry approach based on spontaneous galvanic displacement reaction. We demonstrated that neurons sense and actively respond to the surface nanotopography, with a surprising sensitivity to variations of few nanometers. We showed that focal adhesion complexes, which allow cellular sensing, are strongly affected by nanostructured surfaces, leading to a marked decrease in cell adhesion. Moreover, cells adherent on nanorough surfaces exhibit loss of neuron polarity, Golgi apparatus fragmentation, nuclear condensation, and actin cytoskeleton that is not functionally organized. Apoptosis/necrosis assays established that nanoscale features induce cell death by necrosis, with a trend directly related to roughness values. Finally, by seeding SH-SY5Y cells onto micropatterned flat and nanorough gold surfaces, we demonstrated the possibility to realize substrates with cytophilic or cytophobic behavior, simply by fine-tuning their surface topography at nanometer scale. Specific and functional adhesion of cells occurred only onto flat gold stripes, with a clear self-alignment of neurons, delivering a simple and elegant approach for the design and development of biomaterials with precise nanostructuretriggered biological responses.nanobiointeractions | nanostructures | patterning T he potential of nanomaterials to trigger specific cellular responses, such as interference and/or activation of defined pathways (1-3), is promising for the development of many important scientific fields, such as regenerative medicine, biotechnology, drug delivery, and nanotoxicity assessment. Initially, cellsmaterials interactions were tackled only from a chemical point of view, because environmental sensing by cells involves specific binding between cellular receptors and ECM ligands. However, recently there has been increasing evidence that the biological response is also affected by the physical properties of the material (4). In particular, it has been demonstrated that cells are influenced by the substrate topography (5, 6), rigidity (7, 8), anisotropy (9, 10), surface charge (11, 12), and wettability (13,14). From this perspective, cellular response to external stimuli goes far beyond the bare ability of the cell to chemically sense specific ECM ligands and includes a wide range of physical cues that are generated at, or act on, the interface between cells and the surrounding environment. For instance, it has been observed that micrometer-scale roughness may affect cell proliferation and morphology (15,16), because it provides a quasi-biomimetic microenvironment to the cells. How...

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Characterization of biomaterials polar interactions in physiological conditions using liquid–liquid contact angle measurements

Cited by 33 publications

References 39 publications

Cellular interfacial and surface tensions determined from aggregate compression tests using a finite element model

Cellular interfacial and surface tensions determined from aggregate compression tests using a finite element model

Characterization of the interface between adsorbed fibronectin and human embryonic stem cells

Neurons sense nanoscale roughness with nanometer sensitivity

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