Characterization of bladder calculi and urinalysis in rabbits (Oryctolagus cuniculus) treated with mesenchymal adipose-derived stem cells (ADSCs) after partial urinary bladder allotransplantation

Filho, Saulo Tadeu Lemos Pinto; Dalmolin, Fabíola; Pinho, Renata Medina; Giglio, C; Felin, Diandra V.; Faria, Kamila Lopes de; Vargas, Aline; Brun, Maurício Veloso; Vaz, Marcos André Braz; Pippi, Ney Luís

doi:10.5433/1679-0359.2016v37n2p867

Cited by 3 publications

(3 citation statements)

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“…Urinary stones may be found in the kidneys, urinary bladder, and urinary tract of rabbits of any age and breed (mostly middle-aged and older rabbits) (Figure 2). Rabbit urine is concentrated and sometimes creamy due to its calcium carbonate and ammonium magnesium phosphate crystals and occasionally dark red due to the existence of porphyrin and may be mistaken for hematuria due to blood in the urine (Lee et al, 1978;Pinto Filho et al, 2016). The rabbit's urine pH is alkaline and between 8 and 9.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Hairballs in the Gastrointestinal Tract and Urinary Stones in Razi Institute Laboratory Rabbits

Fallahi,

Dadashpour Davachi

2023

Iran J Vet Med

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Background: Usually, the daily self-grooming by rabbits leads to fur accumulation in the animal’s stomach. Since rabbit hair is looser than other animals and constantly licks their body, the fur can be pulled out easily. On the other hand, rabbits are susceptible to urinary stone formation. Objectives: This study was designed to investigate the presence of hairballs and urinary stones in Razi Institute Laboratory rabbits. Methods: During the 1 year, the albino Dutch laboratory rabbit colony, in research, breeding, and production of the Laboratory Animals Department of Razi Institute, including 106 males, 287 females, and 166 kittens, were monitored. After the necropsy of the selected animals, the gastrointestinal tract (stomach and intestines) were examined for the presence of hair and hairballs. Then the urinary system (kidneys, ureter, urinary bladder, and urethra) was examined for any urinary stones. Results: No symptoms of anorexia, lethargy, abdominal pain, weight loss, decrease and abnormal stools were observed in them, and also no mortality occurred in the whole colony. All samples’ stomach was full, indicating enough eating. No gas or congested spots, or hemorrhage were observed in the intestines. The amount and consistency of stool in the intestines were normal. In none of the samples, hairballs were observed, but in most rabbits’ stomachs (both sexes), a small amount of hair was observed in the stomach contents. Also, no symptoms of urinary stones were observed in the colony of the studied rabbits. Conclusion: Balanced diet, supply of nutritional requirements, and the absence of any stressors in breeding environments have played a key role and prevented many diseases, such as hairballs and urinary stones. No observation of urinary stones in this study could lead to the hypothesis that infection with the bacteria that cause urinary stones in the studied rabbits was eliminated or non-pathogenic, indicating specific pathogen-free animals. However, bacterial and other infectious agent monitoring should be specialized.

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Section: Introductionmentioning

confidence: 99%

Evaluation of the Hairballs in the Gastrointestinal Tract and Urinary Stones in Razi Institute Laboratory Rabbits

Fallahi,

Dadashpour Davachi

2023

Iran J Vet Med

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“…Todavia, quando há menor necessidade ou quando a ingestão de fósforo é restrita, o incremento na excreção renal de cálcio podendo levar à precipitação do mineral em função do pH alcalino, resultando em uma urina densa e cremosa (ECKERMANN-ROSS, 2008;BROWN, 2011). Além disso, cálcio e outros minerais podem formar complexos como fitatos, acetatos e oxalatos, que podem impedir a absorção, levando a formação de urólitos (MEREDITH, 2011;PINTO-FILHO et al, 2016).…”

Section: Introductionunclassified

“…Nos coelhos, os sinais clínicos de urolitíase dependem da localização do urólito e incluem letargia, dor, anorexia, perda de peso, poliúria, anúria, estrangúria, hematúria, postura curvada, bruxismo, polidipsia, incontinência, diminuição da produção fecal, depressão e pododermatite (WHITE, 2001;HARCOURT-BROWN, 2002; BROWN, 2011; QUESENBERRY; CARPENTER, 2012; PROENÇA; MAYER, 2014). Os animais podem apresentar uma combinação de cálculos em localização renal, ureteral, vesical e uretral, e os mais comuns são os vesicais (HARCOURT- BROWN, 2002;QUESENBERRY;CARPENTER, 2012;MACHADO et al, 2014;PROENÇA;MAYER, 2014;PINTO-FILHO et al, 2016;KUCERA et al, 2017).…”

Section: Introductionunclassified

Resolução espontânea de urolitíase uretral peniana severa em um coelho – RELATO DE CASO

Rodrigues¹,

Trentim²,

Lunardelli³

et al. 2019

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RESUMO A urolitíase é uma doença comum em coelhos, caracterizada pela presença de cálculos ou urólitos no sistema urinário. Os cálculos geralmente são constituídos por carbonato de cálcio, sendo os vesicais os mais comuns. O diagnóstico pode ser confirmado por meio de radiografias, onde se identifica o cálculo radiopaco. Devido a um mecanismo de absorção de cálcio exclusivo dos lagomorfos, uma parte importante do tratamento e prevenção desta doença consiste na correção da dieta. O objetivo deste artigo é relatar o caso de um coelho macho, de cinco anos de idade e pesando 2,9kg, que apresentava dificuldade para urinar e defecar, apetite pervertido e urinava em locais não usuais. Ao exame físico, observou-se dor à palpação abdominal, urina concentrada e tenesmo. Foram solicitados exames laboratoriais e radiografia de abdome, encontrando-se na vesícula urinária um urólito liso ovalado medindo 13 mm x 6 mm. O animal não foi submetido a cirurgia, e no decorrer de tratamento clínico o urólito acabou por ser expelido de forma espontânea. PALAVRAS-CHAVE: cálcio, lagomorfos, sistema urinário, urolitíase.

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Expression patterns of mesenchymal stem cell-specific proteins in adipose tissue-derived cells: possible immunosuppressing agent in partial allograft for restoring the urinary bladder in rabbits

et al. 2018

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Adipose tissue-derived stem cells (ADSCs) are an attractive source of mesenchymal stem cells (MSCs) for use in tissue engineering and clinical applications. This paper focuses on the characterization of ADSCs used as immunosuppressive agent in rabbits undergoing partial allograft for urine bladder restorage. For this study highlighted the characterization of the ADSCs used as immunosuppressive agents in rabbits submitted to partial allograft for restoration of the urinary vesicle, using 25 animals, six months old, New Zealand. ADSCs at the third peal were characterized by the MSC-specific CD105, CD73 and CD90 expression and by the absence of the hematopoietic marker CD45, as revealed by flow cytometry analysis. Moreover, ADSCs were efficient in preventing allograft rejection from the urinary bladder, as judged by biochemical, clinical and ultrasonography analysis. Together, these results compose characterization of protein expression profiles and immunosuppressive functionality of ADSCs in rabbits, which had undergone partial allografts of the urinary bladder, foreseeing future applications in clinical practice.

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Characterization of bladder calculi and urinalysis in rabbits (Oryctolagus cuniculus) treated with mesenchymal adipose-derived stem cells (ADSCs) after partial urinary bladder allotransplantation

Cited by 3 publications

References 9 publications

Evaluation of the Hairballs in the Gastrointestinal Tract and Urinary Stones in Razi Institute Laboratory Rabbits

Evaluation of the Hairballs in the Gastrointestinal Tract and Urinary Stones in Razi Institute Laboratory Rabbits

Resolução espontânea de urolitíase uretral peniana severa em um coelho – RELATO DE CASO

Expression patterns of mesenchymal stem cell-specific proteins in adipose tissue-derived cells: possible immunosuppressing agent in partial allograft for restoring the urinary bladder in rabbits

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