Characterization of bone resorbing activity in gingival crevicular fluid from patients with periodontitis

Rasmussen, Lena; Hänström, Lennart; Lerner, Ulf H.

doi:10.1034/j.1600-051x.2000.027001041.x

Cited by 57 publications

(60 citation statements)

References 26 publications

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“…Nishihara et al have proved in a study on mice that IL-1Ra released by cells upon stimulation by lipopolysaccharides of Aggregatibacter (Actinobacillus) actinomycetemcomitans inhibits bone tissue resorption [30]. Similar results were also reported by Oates et al [31], as well as Rasmussen et al [32]. Delima injected human recombinant cytokine IL-1 Ra into interdental papillae in monkeys with experimentally induced periodontitis.…”

Section: Interleukin-1 Receptor Antagonist (Il-1ra)supporting

confidence: 75%

The interleukin-1 receptor antagonist (IL-1-Ra) and soluble tumor necrosis factor receptor I (sTNF RI) in periodontal disease

Słotwińska¹

2013

OJI

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Section: Interleukin-1 Receptor Antagonist (Il-1ra)supporting

confidence: 75%

The interleukin-1 receptor antagonist (IL-1-Ra) and soluble tumor necrosis factor receptor I (sTNF RI) in periodontal disease

Słotwińska¹

2013

OJI

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“…Osteoclast differentiation has been suggested to be initiated by proinflammatory cytokines, including IL-1, tumor necrosis factor alpha (TNF-␣), and IL-6 (29). Elevated expression of IL-1 in periodontal tissue, as well as increased concentrations of the cytokine in gingival crevicular fluid, correlates with disease progression (3,12,30,35,36). Furthermore, IL-1 antagonists inhibit inflammatory-cell recruitment and osteoclast formation, and they prevent the loss of periodontal tissues in primate models of experimental periodontitis (1,8,9,13).…”

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confidence: 99%

Abundant Secretion of Bioactive Interleukin-1β by Human Macrophages Induced byActinobacillus actinomycetemcomitansLeukotoxin

et al. 2005

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Actinobacillus actinomycetemcomitans produces a leukotoxin that selectively kills human leukocytes. Recently, we reported that macrophages are highly sensitive to leukotoxin and that their lysis involves activation of caspase 1. In this study, we show that leukotoxin also induces the production and release of proinflammatory cytokines from human macrophages. The macrophages were challenged with leukotoxin or lipopolysaccharide (LPS) from A. actinomycetemcomitans or LPS from Escherichia coli, and the production and secretion of interleukin-1␤ (IL-1␤), IL-6, and tumor necrosis factor alpha (TNF-␣) were determined at the mRNA and protein levels by reverse transcription-PCR and enzyme-linked immunosorbent assay, respectively. Leukotoxin (1 to 30 ng/ml) induced abundant production and secretion of IL-1␤, while the effects on IL-6 and TNF-␣ production were limited. Leukotoxin (1 ng/ml) caused a 10-times-higher release of IL-1␤ than did LPS (100 ng/ml). The secreted IL-1␤ was mainly the bioactive 17-kDa protein. At higher concentrations (>30 ng/ml), leukotoxin caused secretion of mainly inactive cytokine, the 31-kDa pro-IL-1␤. The presence of specific antibodies to IL-1␤ or of a caspase 1 inhibitor blocked the secretion and production of the cytokine. Supernatants of leukotoxin-challenged macrophages stimulated bone resorption when tested in a mouse calvarial model. The activity could be blocked by an IL-1 receptor antagonist or specific antibodies to IL-1␤. We concluded that A. actinomycetemcomitans leukotoxin can trigger abundant production and secretion of bioactive IL-1␤ by human macrophages, which is mediated by activation of caspase 1.

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“…Different cell types in inflamed and healthy periodontal and peri-implant tissues are capable of producing a variety of important pro-inflammatory and anti-inflammatory cytokines and growth factors which mediate the host response. Interleukin-l /3 (IL-l /3) is able to activate osteoclast cells and consequently is considered one ofthe most important agents responsible for periodontal bone destruction at sites ofperiodontal inflammation (7)(8). IL-6 activates Bvlymphocyte differentiation, 'I'-Iymphocyte proliferation and induces bone resorption (9).…”

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confidence: 99%

Anti-Inflammatory Cytokines in Peri-Implant Soft Tissues: A Preliminary Study on Humans Using CDNA Microarray Technology

et al. 2007

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The mucosa around implants and the gingiva around teeth respond to plaque formation with the development of an inflammatory lesion which has similar magnitude and histological features. Different cell types in inflamed and healthy periodontal and peri-implant tissues are capable of producing a variety of important pro-inflammatory and anti-inflammatory cytokines and growth factors which mediate the host response. The aim of this study is to compare the expression levels of anti-inflammatory cytokines detectable in the peri-implant soft tissue of two single-implant crowns supported either by zirconia or titanium abutments. Two frozen samples of peri-implant soft tissue of two single-implant crowns supported either by zirconia or titanium abutments were treated to obtain mRNA. The mRNA extracted from these specimens was converted in cDNA and analyzed with “SuperArray GEArray Q Series Human Inflammatory Cytokine/Receptor Gene Array kit”, planned for studying 96 genes involved in inflammatory response. Data showed that gene expression levels of anti-inflammatory cytokines were higher in specimens sampled from the zirconia abutment compared with those from the titanium abutment. It was considered important to detect the mRNA levels of the anti-inflammatory mediators in healthy peri-implant tissues to verify the biological tolerability of zirconia compared with titanium abutments. The difference detected in cytokine expression could be due to the intrinsic biological tolerability of zirconia ceramics or to a lesser bacterial accumulation.

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Characterization of bone resorbing activity in gingival crevicular fluid from patients with periodontitis

Abstract: These data show that GCF contains activity(ies) stimulating osteoclastic bone resorption in vitro. The factor primarily responsible for this activity seems to be IL-1alpha, but IL-1alpha is not the sole activator of bone resorption in GCF.

Cited by 57 publications

References 26 publications

The interleukin-1 receptor antagonist (IL-1-Ra) and soluble tumor necrosis factor receptor I (sTNF RI) in periodontal disease

The interleukin-1 receptor antagonist (IL-1-Ra) and soluble tumor necrosis factor receptor I (sTNF RI) in periodontal disease

Abundant Secretion of Bioactive Interleukin-1β by Human Macrophages Induced byActinobacillus actinomycetemcomitansLeukotoxin

Anti-Inflammatory Cytokines in Peri-Implant Soft Tissues: A Preliminary Study on Humans Using CDNA Microarray Technology

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