Characterization of border species among Pythiaceae: several Pythium isolates produce elicitins, typical proteins from Phytophthora spp.

Panabières, Franck; Ponchet, Michel; Allasia, Valérie; Cardin, L.; Ricci, Pierre

doi:10.1017/s0953756297004413

Cited by 73 publications

(59 citation statements)

References 33 publications

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“…Among the human pathogens, elicitins are present only in P. insidiosum and not in true fungi or other microorganisms (30)(31)(32)(33)(34)(35). One of the elicitins, ELI025, has been identified in P. insidiosum as a highly expressed protein on the cell surface (33)(34)(35).…”

Section: Discussionmentioning

confidence: 99%

“…Elicitins form a group of proteins found only in a phylogenetically distinct group of microorganisms, the oomycetes, but are absent in all other microorganisms, including true fungi (30)(31)(32)(33). Recently, we reported a number of elicitins from the P. insidiosum transcriptome, and one of them, ELI025, is highly expressed and appears at the pathogen cell surface (33)(34)(35).…”

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confidence: 99%

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Development of an Anti-Elicitin Antibody-Based Immunohistochemical Assay for Diagnosis of Pythiosis

et al. 2016

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Pythiosis is an emerging and life-threatening infectious disease of humans and animals living in tropical and subtropical countries and is caused by the fungus-like organism Pythium insidiosum. Antifungals are ineffective against this pathogen. Most patients undergo surgical removal of the infected organ, and many die from advanced infections. Early and accurate diagnosis leads to prompt management and promotes better prognosis for affected patients. Immunohistochemical assays (IHCs) have been developed using rabbit antibodies raised against P. insidiosum crude extract, i.e., culture filtrate antigen (CFA), for the histodiagnosis of pythiosis, but cross-reactivity with pathogenic fungi compromises the diagnostic performance of the IHC. Therefore, there is a need to improve detection specificity. Recently, the elicitin protein, ELI025, was identified in P. insidiosum, but it was not identified in other human pathogens, including true fungi. The ELI025-encoding gene was successfully cloned and expressed as a recombinant protein in Escherichia coli. This study aims to develop a new IHC using the rabbit anti-ELI025 antibody (anti-ELI) and to compare its performance with the previously reported anti-CFA-based IHC. Thirty-eight P. insidiosum histological sections stained positive by anti-ELI-based and anti-CFA-based IHCs indicating 100% detection sensitivity for the two assays. The anti-ELI antibody stained negative for all 49 negative-control sections indicating 100% detection specificity. In contrast, the anti-CFA antibody stained positive for one of the 49 negative controls (a slide prepared from Fusarium-infected tissue) indicating 98% detection specificity. In conclusion, the anti-ELI based IHC is sensitive and specific for the histodiagnosis of pythiosis and is an improvement over the anti-CFA-based assay.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Development of an Anti-Elicitin Antibody-Based Immunohistochemical Assay for Diagnosis of Pythiosis

et al. 2016

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“…Polyadenylated RNAs were reverse-transcribed using a SuperScript first-strand synthesis system (Invitrogen, Cergy Pontoise, France) with NotI-oligo(dT) [5Ј-AT TCGCGGCCGCAGGA(T) 16 -3Ј] (25). A PCR was performed on the cDNA template using a combination of the NotI-oligo(dT) primer and degenerate primer 1 (5Ј-ATGAACTTCCGCGCTCTSYTYGC-3Ј), initially designed from conserved sequences of class I elicitins located in the peptide signal region (25). This primer was assumed to efficiently anneal to the peptide signal region of every elicitin class I gene unraveled until now.…”

Section: Methodsmentioning

confidence: 99%

“…Elicitin proteins are restricted to the oomycete genus Phytophthora and a few Pythium species (18,25). They comprise a large family of polypeptides whose intrinsic function remains largely unknown and that can be divided into at least eight classes (18,23).…”

mentioning

confidence: 99%

“…In particular, group I elicitins (ELI-1), characterized by the typical 98-amino-acid elicitin domain, were reported to be the most abundant secreted proteins in culture filtrates (18). Although elicitin genes are not appropriate for phylogenetic studies because of their multigenic family feature, they can be used as a tool for identification purposes since the amino acid sequence of a given elicitin may provide a signature at the species level (25). In addition, the 3Ј untranslated regions (3ЈUTRs) of class I elicitin genes are strictly conserved within an individual species but diverge between species to such an extent that sequence alignment is almost impossible (1,6,11;Panabières et al,unpublished results).…”

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Distribution and Expression of Elicitin Genes in the Interspecific Hybrid OomycetePhytophthora alni

Ioos

Panabières²,

Industri³

et al. 2007

Appl Environ Microbiol

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Phytophthora alni subsp. alni, P. alni subsp. multiformis, and P. alni subsp. uniformis are responsible for alder disease in Europe. Class I and II elicitin gene patterns of P. alni subsp. alni, P. alni subsp. multiformis, P. alni subsp. uniformis, and the phylogenetically close species P. cambivora and P. fragariae were studied through mRNA sequencing and 3 untranslated region (3UTR)-specific PCRs and sequencing. The occurrence of multiple 3UTR sequences in association with identical elicitin-encoding sequences in P. alni subsp. alni indicated duplication/recombination events. The mRNA pattern displayed by P. alni subsp. alni demonstrated that elicitin genes from all the parental genomes are actually expressed in this allopolyploid taxon. The complementary elicitin patterns resolved confirmed the possible involvement of P. alni subsp. multiformis and P. alni subsp. uniformis in the genesis of the hybrid species P. alni subsp. alni. The occurrence of multiple and common elicitin gene sequences throughout P. cambivora, P. fragariae, and P. alni sensu lato, not observed in other Phytophthora species, suggests that duplication of these genes occurred before the radiation of these species.

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Oomycete Genetics and Genomics

Lamour¹,

Kamoun²

2008

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Characterization of border species among Pythiaceae: several Pythium isolates produce elicitins, typical proteins from Phytophthora spp.

Cited by 73 publications

References 33 publications

Development of an Anti-Elicitin Antibody-Based Immunohistochemical Assay for Diagnosis of Pythiosis

Development of an Anti-Elicitin Antibody-Based Immunohistochemical Assay for Diagnosis of Pythiosis

Distribution and Expression of Elicitin Genes in the Interspecific Hybrid OomycetePhytophthora alni

Oomycete Genetics and Genomics

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