Characterization of Botryosphaeriaceae Species as Causal Agents of Trunk Diseases on Grapevines

Carlucci, Antonia; Cibelli, Francesca; Lops, Francesco; Raimondo, Maria Luisa

doi:10.1094/pdis-03-15-0286-re

Cited by 45 publications

(49 citation statements)

References 35 publications

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“…However, despite the extensive symptoms observed after inoculation of isolate LAG05 ( L. viticola ), the amount of extracellular compounds that it produced was very similar to that of the less aggressive isolates. Diplodia seriata is considered either as a saprophytic species or as a weak secondary pathogen of grapevines in many countries (Taylor et al ., ; Carlucci et al ., ), although it is thought to be pathogenic by other authors (Larignon et al ., ; van Niekerk et al ., ). The two isolates used in this study were moderately pathogenic, with variability depending on the cultivar.…”

Section: Discussionmentioning

confidence: 93%

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Life traits of four Botryosphaeriaceae species and molecular responses of different grapevine cultivars or hybrids

et al. 2016

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Botryosphaeriaceae is a fungal family comprising many species involved in botryosphaeria dieback, a worldwide grapevine trunk disease. Currently, the interactions between Botryosphaeriaceae species and various grapevine cultivars are poorly understood and little data is available. This study investigated various life traits of five isolates belonging to four species of Botryosphaeriaceae found in French vineyards (Diplodia mutila, Diplodia seriata, Lasiodiplodia viticola and Neofusicoccum parvum). The two species N. parvum and L. viticola exhibited the highest optimal growth temperature and the best growth rates. They were also responsible for the most extensive necrosis and cankers in three Vitis vinifera cultivars (Cabernet Sauvignon, Merlot and Ugni-Blanc) that differed in susceptibility to botryosphaeria dieback, and in two genotypes resistant to downy and powdery mildew (RV4 and RV5). Identification of the extracellular toxins produced by isolates in culture media showed that the N. parvum isolate had a different metabolite profile from the others, producing terremutin and salicylic acid derivatives, which are known to be compounds associated with virulence. In a second step, life traits were associated with nondestructive monitoring of gene expression involved in the defence mechanisms of five grapevine cultivars and genotypes after inoculation of wood cuttings with Botryosphaeriaceae. The transcript analyses were carried out at different times and were associated with principal component analysis (PCA). Each cultivar presented a specific transcript signature and several transcripts were correlated either with the size of necrosis/cankers or with symptom reduction, thus offering useful markers for breeding or estimating the defence status of plants.

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Section: Discussionmentioning

confidence: 93%

“…In both cases, the wood of the infected grapevines exhibits necrosis (Larignon et al ., ). Botryosphaeria dieback symptoms can lead to wedge‐shaped cankers, necrosis, progressive bud‐break failure and plant dieback (Carlucci et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Life traits of four Botryosphaeriaceae species and molecular responses of different grapevine cultivars or hybrids

et al. 2016

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“…Roesleriaceae P Root rot Japan Kobayashi (2007) Fungal Diversity Qiu et al (2011), Ú rbez-Torres (2011), Diaz et al (2013), Pitt et al (2013Pitt et al ( , 2015, Li et al (2014), Carlucci et al (2015, Pavlic-Zupanc et al (2015), Valencia et al (2015), Comont et al (2016) Teng (1996) Fungal Diversity …”

Section: Microfungi Collected From China Italy Russia and Thailandmentioning

confidence: 99%

Biodiversity of fungi on Vitis vinifera L. revealed by traditional and high-resolution culture-independent approaches

et al. 2018

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This study is unique as it compares traditional and high-resolution culture-independent approaches using the same set of samples to study the saprotrophic fungi on Vitis vinifera. We identified the saprotrophic communities of table grape (Red Globe) and wine grape (Carbanate Gernischet) in China using both traditional and culture-independent techniques. The traditional approach used direct observations based on morphology, single spore isolation and phylogenetic analysis yielding 45 taxa which 19 were commonly detected in both cultivars. The same set of samples were then used for Illumina sequencing which analyzed ITS1 sequence data and detected 226 fungal OTUs, of which 176 and 189 belong to the cultivars Carbanate Gernischet and Red Globe, respectively. There were 139 OTUs shared between the two V. vinifera cultivars and 37 and 50 OTUs were specific to Carbanate Gernischet and Red Globe cultivars respectively. In the Carbanate Gernischet cultivar, Ascomycota accounted for 77% of the OTUs and in Red Globe, almost all sequenced were Ascomycota. The fungal taxa overlap at the genus and species level between the traditional and culture-independent approach was relatively low. In the traditional approach we were able to identify the taxa to species level, while in the culture-independent method we were frequently able to identify the taxa to family or genus level. This is remarkable as we used the same set of samples collected in China for both approaches. We recommend the use of traditional techniques to accurately identify taxa. Culture-independent method can be used to get a better understanding about the organisms that are present in a host in its natural environment. We identified primary and secondary plant pathogens and endophytes in the saprotrophic fungal communities, which support previous observations, that dead plant material in grape vineyards can be the primary sources of disease. Finally, based on present and previous findings, we provide a worldwide checklist of 905 fungal taxa on Vitis species, which includes their mode of life and distribution.

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“…Javadi & A.J.L. Phillips (Carlucci et al, 2015)]. Again, we recognize that a single locus may not discriminate all species, hence our inclusion of secondary 'group-specific' barcodes in TrunkDiseaseID, which are often found to be more informative (i.e., variable) than ITS in distinguishing some genera or species of trunk pathogens [e.g., EF1-a (Úrbez-Torres et al, 2015)].…”

Section: Trunk Pathogens For Which Molecular Identification Outperformentioning

confidence: 99%

TrunkDiseaseID.org: A molecular database for fast and accurate identification of fungi commonly isolated from grapevine wood

et al. 2017

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The grapevine trunk-disease complex limits vineyard longevity in all major grape-growing regions. Although trunk diseases have been distinguished based on etiologies (e.g., Botryosphaeria-, Eutypa-, and Phomopsis dieback, Esca) and causal agents, mixed infections of trunk pathogens and other woodcolonizing fungi are frequent in grapevines. These diverse fungal communities in grapevine span four classes in the Pezizomycotina (Ascomycota) and 10 genera in the Hymenochaetales (Basidiomycota). Traditional identification based on morphology is largely untenable because of overlap in colony characteristics or spore dimensions, or lack of sporulation in culture. When based on DNA sequencing, searches of uncurated, public molecular databases can lead to misidentifications. The new molecular database TrunkDiseaseID.org is populated with accurate rDNA ITS sequences from 250 þ isolates (pathogens and saprobes) and secondary DNA barcodes for delineating closely related species. Currently, no such comprehensive database exists for grapevine wood-colonizing fungi. In addition to ITS and secondary barcode sequences, this database provides a scientific reference, origin, and ecological status for each isolate, to aid diagnosticians in communicating results and recommendations to growers. Outreach through the National Plant Diagnostic Network and the US Department of Agriculture's Identification Technology Program will connect diagnosticians to this new database for grape. Published by Elsevier Ltd.

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Characterization of Botryosphaeriaceae Species as Causal Agents of Trunk Diseases on Grapevines

Cited by 45 publications

References 35 publications

Life traits of four Botryosphaeriaceae species and molecular responses of different grapevine cultivars or hybrids

Life traits of four Botryosphaeriaceae species and molecular responses of different grapevine cultivars or hybrids

Biodiversity of fungi on Vitis vinifera L. revealed by traditional and high-resolution culture-independent approaches

TrunkDiseaseID.org: A molecular database for fast and accurate identification of fungi commonly isolated from grapevine wood

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