Characterization of bovine microsatellites by silver staining

Comincini, Sergio; Leone, P.; Redaelli, Loredana; Giuli, L. de; Zhang, Y.; Ferretti, Luca

doi:10.1111/j.1439-0388.1995.tb00580.x

Cited by 19 publications

(15 citation statements)

References 14 publications

(16 reference statements)

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“…After digestion with the appropriate restriction enzyme, fragments were analyzed in 3% agarose gels in 1X TBE buffer, stained with ethidium bromide and photographed using an EDAS (Kodak TM ) digital system. For microsatellite loci the cycles were the same as described by Bishop et al (1994) and the PCR products were analyzed in 8% polyacrylamide gels in a manual sequencing apparatus, followed by silver staining (Comincini et al, 1995). Genotypes for the locus BM7160 were determined with an automatic laser fluorescence DNA sequencer (Pharmacia Biotech).…”

Section: Genotypingmentioning

confidence: 99%

Genetic characterization of Aberdeen Angus cattle using molecular markers

et al. 2003

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Aberdeen Angus beef cattle from the Brazilian herd were studied genetically using restriction fragment length polymorphism (RFLP) of the κ-casein -HinfI (CSN3 -HinfI), β-lactoglobulin -HaeIII (LGB -HaeIII) and growth hormone AluI (GH-AluI) genes, as well as four microsatellites (TEXAN15, CSFM50, BM1224 and BM7160). The RFLP genotypes were determined using the polymerase chain reaction (PCR) followed by digestion with restriction endonucleases and electrophoresis in agarose gels. With the exception of the microsatellite BM7160, which was analyzed in an automatic sequencer, the PCR products were genotyped by silver staining. The allele and genotype frequencies, heterozygosities and gene diversity were estimated. The values for these parameters of variability were comparable to other cattle breeds. The genetic relationship of the Aberdeen Angus to other breeds (Caracu, Canchim, Charolais, Guzerath, Gyr, Nelore, Santa Gertrudis and Simmental) was investigated using Nei's genetic distance. Cluster analysis placed the Aberdeen Angus in an isolated group in the Bos taurus breeds branch. This fact is in agreement with the geographic origin of this breed.

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Section: Genotypingmentioning

confidence: 99%

Genetic characterization of Aberdeen Angus cattle using molecular markers

et al. 2003

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“…Os oligonucleotídeos complementares à fita anti-sense, denominado primer forward, foram marcados com fluoresceína na extremidade 5'. Os fragmentos amplificados do marcador microssatélite CSFM50 foram separados em géis de poliacrilamida 6,0% e analisados pelo método de coloração com prata (COMINCINI et al, 1995).…”

Section: Condições Da Reação De Pcrunclassified

Caracterização molecular de animais da raça Nelore utilizando microssatélites e genes candidatos

et al. 2000

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RESUMO -No presente trabalho, 180 fêmeas da raça Nelore, provenientes de oito rebanhos, foram analisadas quanto aos marcadores microssatélites TEXAN15, BM1224 e CSFM50 e aos polimorfismos de comprimento de fragmentos de restrição (RFLP) nos locos κ-caseína, β-lactoglobulina e hormônio de crescimento (GH). Com exceção de GH, todos os marcadores foram polimórficos na amostra estudada. Os valores de heterozigosidade, diversidade gênica, conteúdo de informação polimórfica (PIC) e probabilidade de exclusão de paternidade (PE) foram estimados. Os maiores valores de PIC (0,685) e PE (0,521) foram obtidos para o marcador BM1224.Palavras-chave: bovino, DNA, marcador, microssatélite, Nelore, RFLP Molecular Characterization of a Nellore Beef Cattle Sample Using Microsatellites and Candidate GenesABSTRACT -In the present work, 180 Nellore females from eight herds were analyzed for TEXAN15, BM1224 and CSFM50 microsatellites and for the restriction fragments polymorphism (RFLP) at the locos κ-casein, β-lactoglobulin and growth hormone (GH). Excepted for GH, all markers were polymorphic in the studied sample. The values of heterozigozity, gene diversity, polymorphic information content (PIC) and paternity exclusion probability (PE) were estimated. The highest PIC (0.685) and PE (0.521) values were obtained for microsatellite BM1224.Key Words: bovine, DNA, marker, microsatellite, Nelore, RFLP 1 Departamento de Genética e Evolução -UFSCar, São Carlos -SP. E.mail: jtalhari@terra.com.br; matamb@terra.com.br 2 EMBRAPA -CPPSE, São Carlos, SP. E.mail: mauricio@cppse.embrapa.br; tambasco@zaz.com.br; luciana@cppse.embrapa.br 3 Departamento de Produção Animal -ESALQ, Piracicaba -SP. E.mail: llcoutin@carpa.ciagri.usp.br IntroduçãoDevido a sua importância econômica e cultural, estudos das relações genéticas entre rebanhos bovinos têm sido, historicamente, uma área ativa da sistemá-tica biológica. A caracterização de determinada raça pode ser feita por suas propriedades fenotípicas, morfológicas e fisiológicas, origem, habitat, distribuição geográfica, produção e parâmetros genéticos

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“…Thermal cycling conditions were as follows: 5 min at 95ºC, 35 cycles of 1 min at 94ºC, 1 min at 56ºC (for all loci), 1 min at 72ºC, and a final extension of 20 min at 72ºC. Amplified products were resolved on 7% silver-stained denaturing polyacrylamide gel (Comincini et al, 1995). Alleles were scored by comparison with a 10 bp DNA ladder (Fermentas Life Sciences).…”

Section: Laboratory Proceduresmentioning

confidence: 99%

Genetic population structure of two migratory freshwater fish species (Brycon orthotaenia and Prochilodus argenteus) from the Sao Francisco River in Brazil and its significance for conservation

Sanches

Galetti

Galzerani

et al. 2012

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ABSTRACT. Previous genetic studies conducted with migratory fish populations from downstream of the Três Marias dam in the São Francisco River Basin (Brazil) have documented the occurrence of population structuring, as reported for Brycon orthotaenia and Prochilodus argenteus, two commercially important species in this basin. We revisited the genetic structure of these species using microsatellites. B. orthotaenia was sampled during the spawning season and was analyzed using five heterologous microsatellites. P. argenteus was collected in the non-reproductive season and genetic analysis was conducted using ten speciesspecific microsatellites. For both species, genetic diversity between collection sites was similar. Considering B. orthotaenia, F ST and R ST estimates and the Bayesian analysis demonstrated significant differences between sites. Two well-defined populations were identified in the study area, indicating population structuring for this species. No significant differences were found for P. argenteus. These data provide information for knowledge regarding genetic structure of migratory fish species, which may contribute toward the conservation besides the understanding the biology and ecology of these important fishery resources. Keywords: migratory fish, freshwater fish, genetic structure, Characidae, Prochilodontidae, microsatallite, Brazil.Estructura genética poblacional de dos especies de peces migratorios de agua dulce (Brycon orthotaenia y Prochilodus argenteus) en la cuenca del Río San Francisco (Brasil) y su importancia para la conservación RESUMEN. Estudios genéticos anteriores realizados con poblaciones de peces migratorios de aguas abajo de la represa de las Tres Marías en la cuenca del río San Francisco (Brasil) han documentado casos de estructuración genética, como se ha descrito para Brycon orthotaenia y Prochilodus argenteus, dos especies de importancia comercial. Se revisó la estructura genética de estas especies utilizando microsatélites. Se obtuvieron muestras de B. orthotaenia durante la temporada de desove y se analizaron mediante cinco microsatélites heterólogos. Muestras de P. argenteus fueron recogidas en la temporada no reproductiva y el análisis genético se realizó utilizando diez microsatélites específicos para P. argenteus. Para ambas especies, la diversidad genética entre los sitios de recolección fue similar. Considerando a B. orthotaenia, las estimaciones F ST y R ST y el análisis Bayesiano demostraron diferencias significativas entre los sitios. Se identificaron dos poblaciones bien definidas en el área de estudio, indicando una estructuración de la población de esta especie. No se encontraron diferencias significativas para P. argenteus. Estos datos proporcionan información para el conocimiento sobre la estructura genética de las especies de peces migratorios, que puede contribuir a la conservación, además de la comprensión de la biología y ecología de estos importantes recursos pesqueros. Palabras clave: peces migratorios, peces de agua dulce, estructura genética, Chara...

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Characterization of bovine microsatellites by silver staining

Cited by 19 publications

References 14 publications

Genetic characterization of Aberdeen Angus cattle using molecular markers

Genetic characterization of Aberdeen Angus cattle using molecular markers

Caracterização molecular de animais da raça Nelore utilizando microssatélites e genes candidatos

Genetic population structure of two migratory freshwater fish species (Brycon orthotaenia and Prochilodus argenteus) from the Sao Francisco River in Brazil and its significance for conservation

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