Characterization of buffalo interleukin 8 (IL-8) and its expression in endometritis

Abstract

River buffalo (Bubalus bubalis bubalis) with a population over 135 million heads is an important livestock. Interleukin 8 (IL-8) is a member of the chemokine family and is an important chemoattractant for neutrophils associated with a wide variety of inflammatory diseases such as endometritis. Tissue samples from the mammary gland, uterus and ovary were obtained from river buffalo (Mediterranean type) with and without endometritis. Bacteriological examination showed the presence of both gram positive and negative in all buffalo with endometritis. RNA extraction and complementary DNA (cDNA) synthesis were conducted from all tissues. Specific primer for IL8 full coding regions was designed using known cDNA sequences of Bubalus bubalis, Genbank accession number AY952930.1. IL-8 gene expression was investigated in buffalo tissues. Expression of IL-8 in buffalo with endometritis was found to increase significantly over buffalo without endometritis only in the uterus (P = 0.0159). PCR products from uterus tissues (target organs) of buffalo with and without endometritis, were purified and sequenced. No polymorphic sites were detected in the investigated samples. IL-8 cDNA nucleotide sequences of buffalo with and without endometritis were 100% identical (accession number JX413057). Buffalo IL8 cDNAs were compared with corresponding sequences of member of subfamily Bovinae (buffalo and cattle) and subfamily Caprinae (sheep and goat). IL-8 species specific differences were identified.

Chemotactic factor inducing Interleukin-8 (IL8) gene is transcriptionally elevated in experimental enterotoxaemia in goats caused by Clostridium perfringens type D

“…IL-8 is an important mediator for innate immunity in mammals and involved in recruitment and activation of different leukocytes through modulating cytokine production ( Min et al., 2001 ). Similar findings were reported in another study, where IL8 was up-regulated significantly (P < 0.0159) to 26.6 fold in uterine tissue of buffaloes with endometritis when compared to the healthy uterine tissue ( Mossallam et al., 2015 ). The current study showed higher expression of IL-8 mRNA in CS group compared to all other groups studied, which may be due to the presence of bacterial secretory components in excess that increases the possibility of IL-8 signalling to attract neutrophils to the area of infection.…”

Subclinical endometritis and postpartum ovarian resumption in respect to TNF-±, IL-8 and CRP in Egyptian buffaloes

“…The observed elevation in IL-8 levels augmented the elevation of TNF-α that induced its production and further uterine PMNs chemotaxis (Manimaran et al, 2016;Nehru et al, 2019). Similar findings were obtained by Mossallam et al (2015) who recorded significant increase in the IL-8 levels in uterine tissue of SCE buffaloes. Furthermore, Singh (2017) observed increments in both circulating and endometrial IL-8 levels in SCE buffaloes when compared to control group.…”

“…Tumor necrosis factor alpha, multifunctional pro-inflammatory cytokine, secreted by many cells as macrophages, T lymphocytes and ovarian granulosa cells (Price and Sheldon, 2013). Interleukine-8, the primary neutrophil chemokine, is produced by activated leukocytes that enhance the targeted release of neutrophils and T lymphocytes in order to eliminate pathogens in bovine uterus (Bhadaniya et al, 2019;Mossallam et al, 2015). Both TNF-α and IL-8 are essential in uterine immune cascade of SCE buffaloes via their upregulation in the endometrium (Loyi et al, 2013).…”

Ovulatory follicular fluid induces sperm phagocytosis by neutrophils, but oviductal fluid around oestrus suppresses its inflammatory effect in the buffalo oviduct in vitro

“…IL‐8 is a proinflammatory chemokine that regulates recruitment and trafficking of neutrophils (Neuman et al, ). The expression of IL‐8 was detected in mammary glands, uteri, and ovaries of buffalo with and without inflammation (Abou Mossallam, El Nahas, Mahfouz, & Osman, ). Moreover, IL‐8 was found in the preovulatory follicle, and its role in folliculogenesis was reported (Gazvani et al, ).…”