Characterization of C₆₀ fullerene complexation with antibiotic doxorubicin

Abstract

The aim of this paper was to provide the physico-chemical characterization of a key process leading to amplification of the antitumor effect of antibiotic Doxorubicin (Dox) in vivo and in vitro and occurring at the molecular level through complexation with C60 fullerene. A wide range of physico-chemical tools was used such as UV/Vis and NMR spectroscopies, atomic force microscopy, isothermal titration calorimetry and zeta-potential methods. The unusual thermodynamic behavior of the complexation process was reported, featuring unexpected and, to a certain extent, contradictory experimental observations. The explanation of the obtained results was proposed resulting in creation of a general view on aromatic drug binding with C60 fullerene. Based on these results some important practical outcomes for anticancer therapy were formulated.

Biophysical characterization of the complexation of C60 fullerene with doxorubicin in a prokaryotic model

“…This result may be interpreted as a consequence of existence of at least two statistically different entities in solution, which are the C 60 fullerene aggregates (the first maximum) and the complexes between the C 60 and doxorubicin molecules (the second maximum) . This result is in agreement with previous spectroscopic investigation of C 60 + doxorubicin interaction in water solution and provides independent confirmation to the fact of complexation .…”

Fullerene as a doxorubicin nanotransporter for targeted breast cancer therapy: Capillary electrophoresis analysis

“…Prylutskyy et al. reported a value of ‒28 mV. In contrast, some authors found different values; for instance, Brant et al.…”

C60 fullerene affects elastic properties and osmoregulation reactions of human lymphocytes

“…In order to utilize transport properties of C 60 fullerene, as a rule, it has to be chemically modified by attachment to the drug itself or other compounds (Zakharian et al 2005;Liu et al 2010). However, recently the ability of pristine C60 fullerene dissolved in water solution (to be further referred to as C 60 FAS) to act as a carrier due to non-covalent complexation with drug was reported Prylutskyy et al 2014a). This fact is manifested in the marked enhancement of the anti-tumor effect of the antibiotic doxorubicin in vitro and in vivo in the presence of C 60 fullerene (Panchuk et al 2015;Prylutska et al 2014), and makes the development of new improved regimes of combined C 60 fullerene chemotherapy potentially possible.…”