Characterization of canine oral papillomavirus by histopathological and genetic analysis in Korea

Yhee, Ji Young; Kwon, Byoung Mog; Kim, Jong Hyuk; Yu, Chi Ho; Im, Keum Soon; Lee, Sung Seok; Lyoo, Young S.; Chang, Byung Joon; Sur, Jung Hyang

doi:10.4142/jvs.2010.11.1.21

Cited by 12 publications

(17 citation statements)

References 17 publications

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“…Confirmation of the participation of CPV1 within these lesions was obtained due to the successful amplification of viral DNA with subsequent sequencing of the partial L1 gene of CPV1; similar results have been described (Yhee et al 2010). However, it must be highlighted that the degenerate primers (FAP 59 and FAP 64), originally designed to detect a broad range of cutaneous HPVs (Forslund et al 1999), successfully amplified the partial fragment of the CPV1 L1 gene, suggesting that direct sequencing is an important tool for the molecular characterization of PV types of infectious agents.…”

Section: Discussionsupporting

confidence: 63%

“…Similar histopathological findings were recently described in dogs with oral papillomavirus from Korea identified by molecular biology (Yhee et al 2010) and in dogs from Brazil characterized by immunohistochemistry for Papillomavirus (Bianchi et al 2012), however the last investigation did not specify the PV type identified in dogs from Brazil.…”

Section: Discussionsupporting

confidence: 63%

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Identification of canine papillomavirus type 1 (CPV1) DNA in dogs with cutaneous papillomatosis

et al. 2014

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Canine oral papillomavirus (COPV), also known as Canine Papillomavirus type 1 (CPV1), induces papillomas at the mucous membranes of the oral cavity and at the haired skin of dogs. The classification of Papillomavirus (PV) types is based on the L1 capsid protein and nucleotide sequence; so far, 14 CPV types have been described in several countries, but the molecular characterization of CPV in Brazil is lacking. This study investigated the presence of the PV in seven papillomas from four mixed breed dogs from Londrina/PR, Southern Brazil, by partial sequencing of the L1 gene. Seven exophytic cutaneous lesions were surgically removed and processed for histopathological and molecular characterization. Histopathology confirmed the lesions as viral papillomas due to typical histological features. Polymerase Chain Reaction (PCR) assay using the FAP59 and FAP64 primers targeted the L1 gene followed by sequence analysis of the amplicons identified CPV1 in all evaluated papilloma samples. This study represents the first description of CPV1 DNA associated with canine papillomatosis in Brazil.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionsupporting

confidence: 63%

Identification of canine papillomavirus type 1 (CPV1) DNA in dogs with cutaneous papillomatosis

et al. 2014

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“…The same immunostaining was seen in an outbreak of canine oral papillomavirus in Korea (Yheel et al 2010). This antibody targets the whole virus, which is formed only in differentiated squamous epithelial cells.…”

Section: Discussionsupporting

confidence: 60%

Canine papillomatosis: a retrospective study of 24 cases (2001-2011) and immunohistochemical characterization

et al. 2012

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A retrospective study of 24 cases of papillomas in dogs was performed from January 2001 to March 2011. Additionally, immunohistochemistry (IHC) was used to characterize and evaluate the samples. We found that disease was observed more in mixed breed dogs, ages ranging from 6 months to 10 years (mean 3.1 years), and there was no gender predilection. The main lesion sites were the skin (75%), lips (16.7%), and eyelids (8.3%). Upon histological evaluation, we observed papillary exophytic proliferation of squamous epithelium and papillary endophytic proliferation (inverted) in 87.5% and 12.5% of cases, respectively. The tumors were characterized by spinous layer hyperplasia (87.5%) with koilocytes (70.8%) and intranuclear pale basophilic inclusions bodies (8.3%), prominent granular layer with large amounts of keratohyalin granules (95.8%), and hyperkeratosis in the stratum corneum (100%). Positive immunostaining for Papillomavirus was found in 83.3% of cases, which were distributed between the granular layer and the stratum corneum. These findings indicate the following: that papillomas in dogs are caused by Papillomavirus, the viral cytopathic effect induces epithelial lesions, viral particles are found inside the cell nuclei, and inclusions bodies are rare.

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“…Microscopic examination of stained section is characterized by presence of prominent epithelial proliferations with thickened epidermis. The keratinocytes present in the stratum granulosum or spinosum had prominent nucleus (Yhee et al, 2010). Canine papillomatosis can also be effectively managed medically using different drugs.…”

Section: Discussionmentioning

confidence: 99%

“…Azithromycin is considered as a safe and effective therapeutic agent for the management of papillomatosis in canines. It is used at a dose rate of 10 mg/kg every 24 hour for a period of 10 days for the management of oral and cutaneous canine papillomatosis (Yaǧcı et al, 2008). Recombinant feline interferon-ω is a good choice for the management of canine papillomatosis cases, which are not responsive to other modes of therapy (Fantini et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Oral Papillomatosis in a Dog: Surgical Management and Histopathological Findings

Sharun¹,

Kalaiselvan²,

Melanthota³

et al. 2020

Adv. Anim. Vet. Sci.

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| Canine oral papillomatosis is a viral disease that commonly affects young dogs. A six-month-old male German shepherd dog was presented with a history of progressively developed nodular growth in the lip, gingiva and tongue. All physical parameters were found to be normal. The animal was pre-medicated using atropine, butorphanol and diazepam as per the standard pre-anesthetic protocol. General anaesthesia was induced using ketamine hydrochloride. General maintenance was provided using Ketamine-diazepam mixture and the nodular masses were removed using a combination of surgical excision and electrocautery. Post-operatively, the animal was treated with antibiotics for five days and anti-inflammatory drugs for three days. Excised tissue samples were processed and subjected to standard histopathological examination. Histopathologic examination revealed a diffuse epidermal hyperplasia, marked neovascularization, koilocytes with clear perinuclear vacuolization and keratinocytes with keratohyalin granules. The findings were suggestive of canine oral papilloma virus induced papillomatosis. The animal made an uneventful recovery without any recurrence. This paper describes the successful surgical management of canine oral papillomatosis without any recurrence and its peculiar histopathological findings.

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Characterization of canine oral papillomavirus by histopathological and genetic analysis in Korea

Cited by 12 publications

References 17 publications

Identification of canine papillomavirus type 1 (CPV1) DNA in dogs with cutaneous papillomatosis

Identification of canine papillomavirus type 1 (CPV1) DNA in dogs with cutaneous papillomatosis

Canine papillomatosis: a retrospective study of 24 cases (2001-2011) and immunohistochemical characterization

Oral Papillomatosis in a Dog: Surgical Management and Histopathological Findings

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