Characterization of Carotenoid-protein Complexes and Gene Expression Analysis Associated with Carotenoid Sequestration in Pigmented Cassava (Manihot Esculenta Crantz) Storage Root

Carvalho, Luiz Joaquim Castelo Branco

doi:10.2174/1874091x01206010116

Cited by 26 publications

(13 citation statements)

References 37 publications

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“…It was reported that the HSPs were involved in response to environmental stress such as heat, cold, drought and salinity stress [56, 57]. In plants, the HSPs have been speculated to act as an antioxidant under oxidative stress [58] or as an important tool for genetic manipulation of protein content in cassava storage root contributing to a natural sink for protein and carotenoid accumulation in intense yellow roots [59]. Further elucidation of the roles of HSPs in binding specifically with PPD would provide our understanding of the molecular machinery controlling PPD onset.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of injured storage roots in cassava (Manihot esculenta Crantz) under postharvest physiological deterioration

Qin

Djabou

et al. 2017

PLoS ONE

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Postharvest physiological deterioration (PPD) is a global challenge in the improvement of cassava value chain. However, how to reduce cassava spoilage and reveal the mechanism of injured cassava storage roots in response to PPD were poorly understood. In the present study, we investigated the activities of antioxidant enzymes of cassava injured storage roots in PPD-susceptible (SC9) and PPD-tolerant (QZ1) genotypes at the time-points from 0h to 120h, and further analyzed their proteomic changes using two-dimensional electrophoresis (2-DE) in combination with MALDI-TOF-MS/MS. Ninety-nine differentially expressed proteins were identified from SC9 and QZ1 genotypes in the pairwise comparison of 24h/0h, 48h/0h, 72h/0h and 96h/0h. Of those proteins were associated with 13 biological functions, in which carbohydrate and energy metabolism related proteins were the biggest amount differential proteins in both genotypes, followed by chaperones, DNA and RNA metabolism, and defense system. We speculated that SOD in combination with CAT activities would be the first line of defense against PPD to support PPD-tolerant cassava varieties. The four hub proteins including CPN60B, LOS2, HSC70-1 and CPN20B, produced from the network of protein-protein interaction, will be the candidate key proteins linked with PPD. This study provides a new clue to improve cassava PPD-tolerant varieties and would be helpful to much better understand the molecular mechanism of PPD of cassava injured storage roots.

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Section: Discussionmentioning

confidence: 99%

Proteomic analysis of injured storage roots in cassava (Manihot esculenta Crantz) under postharvest physiological deterioration

Qin

Djabou

et al. 2017

PLoS ONE

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“…The 26.4 KDa heat-shock protein (A5A8U9), which was present in the line with the addition of 7 H ch could also be playing a key role in the accumulation of carotenoids as small heat shock proteins were found to be the most abundant proteins present in the carotenoid-protein complexes of cassava roots, suggesting their involvement in the accumulation of these pigments. [ 70 ].…”

Section: Discussionmentioning

confidence: 99%

Novel Bread Wheat Lines Enriched in Carotenoids Carrying Hordeum chilense Chromosome Arms in the ph1b Background

et al. 2015

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The use of crop wild relative species to improve major crops performance is well established. Hordeum chilense has a high potential as a genetic donor to increase the carotenoid content of wheat. Crosses between the 7Hch H. chilense substitution lines in wheat and the wheat pairing homoeologous1b (ph1b) mutant allowed the development of wheat-H. chilense translocation lines for both 7Hchα and 7Hchβ chromosome arms in the wheat background. These translocation lines were characterized by in situ hybridization and using molecular markers. In addition, reverse phase chromatography (HPLC) analysis was carried out to evaluate the carotenoid content and both 7Hchα∙7AL and 7AS∙7Hchβ disomic translocation lines. The carotenoid content in 7Hchα∙7AL and 7AS∙7Hchβ disomic translocation lines was higher than the wheat-7Hch addition line and double amount of carotenoids than the wheat itself. A proteomic analysis confirmed that the presence of chromosome 7Hch introgressions in wheat scarcely altered the proteomic profile of the wheat flour. The Psy1 (Phytoene Synthase1) gene, which is the first committed step in the carotenoid biosynthetic pathway, was also cytogenetically mapped on the 7Hchα chromosome arm. These new wheat-H. chilense translocation lines can be used as a powerful tool in wheat breeding programs to enrich the diet in bioactive compounds.

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“…source of starch, but not of protein and vitamins (CARVALHO et al, 2012). However, the pink root has the genetic potential to become a source of lycopene for human consumption (CARVALHO et al, 2016).…”

Section: Crop Productionmentioning

confidence: 99%

“…(Table 4), with the 2010/2011 crop season highlighting clone 406/08 (39.19 μg g -1 dry mass) and the 2011 / 2012 crop season the clone 341/08 (42.80 μg g -1 of dry mass) (Table 4). The higher absolute values of total carotenoids in clones with pink root color compared to cream and yellow are probably due to the fact that they accumulate lycopene and β-carotene in their reserve roots (CARVALHO et al, 2012;SILVA et al, 2014). Regarding the hydrocyanic acid (HCN) content in the cassava stock roots, all clones presented amounts below 100 mg kg -1 (Table 3); and are therefore, suitable for to being commercialized fresh.…”

Section: Fv Gl ---------------------------------------------------Qm-mentioning

confidence: 99%

Agronomic and biochemical evaluation of cassava clones with roots that have pink pulp

et al. 2020

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Sweet cassava breeding programs are focused on the development of bio fortified cultivars that combine significant amounts of carotenoids in their reserve roots with desirable agronomic. The objective of this research was to evaluate agronomic and biochemical traits in sweet cassava clones with roots that have pink pulp. The nine genotypes were evaluated in two seasons in a randomized block design with three replications. Among the evaluated clones, the following stood out: i) for the height of the first branch (390/08, 345/08 and the control IAC 576-70); ii) for plant height (390/08, 345/08 e 378/08); iii) for shoot weight without original steam cutting (390/08, 406/08, 378/08 e 341/08); iv) for the percentage of starch in roots (378/08, 413/08, 390/08 and the control IAC 576-70); and v) for the root yield (the control IAC 576-70 and 341/08, 390/08, 406/08 e 387/08). In the 2011/2012 season, all clones cooked within 30 minutes, indicating that they all have good culinary qualities. Regarding the total carotenoid content in the roots, the clones that stood out were 406/08 and 341/08. All clones evaluated had HCN content below 100 mg kg-1. Clones 341/08 and 406/08 have agronomic and biochemical potential for direct cultivation by producers in the Cerrado region of Central Brazil and / or for use as stock in sweet cassava breeding programs.

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Characterization of Carotenoid-protein Complexes and Gene Expression Analysis Associated with Carotenoid Sequestration in Pigmented Cassava (Manihot Esculenta Crantz) Storage Root

Cited by 26 publications

References 37 publications

Proteomic analysis of injured storage roots in cassava (Manihot esculenta Crantz) under postharvest physiological deterioration

Proteomic analysis of injured storage roots in cassava (Manihot esculenta Crantz) under postharvest physiological deterioration

Novel Bread Wheat Lines Enriched in Carotenoids Carrying Hordeum chilense Chromosome Arms in the ph1b Background

Agronomic and biochemical evaluation of cassava clones with roots that have pink pulp

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