2014
DOI: 10.1016/j.jprot.2014.01.005
|View full text |Cite
|
Sign up to set email alerts
|

Characterization of carp seminal plasma proteome in relation to blood plasma

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

3
37
1

Year Published

2015
2015
2022
2022

Publication Types

Select...
4
2

Relationship

1
5

Authors

Journals

citations
Cited by 58 publications
(41 citation statements)
references
References 46 publications
3
37
1
Order By: Relevance
“…Specific isoforms of apolipoproteins were also detected: Apolipoproteins A‐I, A‐II, B and C were previously identified in fish blood (Loir et al, ), while apolipoproteins A and C were reported in fish seminal plasma (Babin and Vernier, ; Ciereszko et al, ); this is the first study to identify multiple isoforms of apolipoprotein E in carp seminal plasma. This new finding indicates that different testis‐specific isoforms are expressed and/or the presence of potential post‐translational modifications (Dietrich et al, ). The variety of distinct apolipoprotein isoforms in carp seminal plasma are likely linked to sperm energy resources and the maintenance of spermatozoon membrane lipid composition.…”
Section: Discussionmentioning
confidence: 89%
“…Specific isoforms of apolipoproteins were also detected: Apolipoproteins A‐I, A‐II, B and C were previously identified in fish blood (Loir et al, ), while apolipoproteins A and C were reported in fish seminal plasma (Babin and Vernier, ; Ciereszko et al, ); this is the first study to identify multiple isoforms of apolipoprotein E in carp seminal plasma. This new finding indicates that different testis‐specific isoforms are expressed and/or the presence of potential post‐translational modifications (Dietrich et al, ). The variety of distinct apolipoprotein isoforms in carp seminal plasma are likely linked to sperm energy resources and the maintenance of spermatozoon membrane lipid composition.…”
Section: Discussionmentioning
confidence: 89%
“…Similarly to rainbow trout, the quantitative comparison of proteome profile between SP and BP performed using 2D‐DIGE revealed that the majority of carp SP proteins are also present in BP . There is substantial overlap in the composition of the major proteins of carp and rainbow trout, suggesting that the general protective mechanisms in teleosts are well conserved.…”
Section: Discussionmentioning
confidence: 99%
“…The apparent duplication of many of the teleost genes contributes to the complexity of the fish immune system. So far, LC‐MS/MS has been applied to carp BP and has led to the identification of 88 plasma proteins . Although studies leading to the identification of a few or several blood proteins have been undertaken in some salmonid species , there is no comprehensive portrait of the salmonid BP proteome, which is needed to understand the molecular mechanisms taking place in the blood, particularly those related to immune reactions.…”
Section: Introductionmentioning
confidence: 99%
“…Mass spectrometric experiments were performed using a nano LC LTQ-Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) equipped with a nano-electrospray ion source and an Easy II nano LC, as described previously with some modifications28. Animal horn samples were divided into soluble and insoluble fractions as described above, and 5 μL samples (1 μg of total protein) were loaded onto a self-packed 5 μm Reprosil C18AQ column (75 μm × 150 mm).…”
Section: Methodsmentioning
confidence: 99%
“…All MS/MS samples were analyzed using X!Tandem (thegpm.org; version TORNADO 2010.01.01.4) which was set up to search the bovidae_uniprot-taxonomy_9895 database (downloaded on the 26 th April, 2014). Spectra were also searched against an equal number of decoy sequences to estimate the false discovery rate (FDR) as described previously28. The specified enzyme was trypsin and up to two missed cleavages were allowed.…”
Section: Methodsmentioning
confidence: 99%