Characterization of CBL-Interacting Protein Kinases’ Gene Family and Expression Pattern Reveal Their Important Roles in Response to Salt Stress in Poplar

Bai, Xiaoyong; Ji, Jiabao; Wang, Wei; Gu, Chao; Yu, Qibin; Jiang, Jing; Yang, Chuanping

doi:10.3390/f13091353

Cited by 5 publications

(4 citation statements)

References 35 publications

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“…However, our study found that GhCIPK23 showed a different expression pattern under salt stress than under other abiotic stresses and that this gene was significantly downregulated within the salt stress treatment period. The same results were observed in poplar and wheat [ 37 , 38 ]. In poplar, the gene expression of PtrCIPK1 , PtrCIPK5 , PtrCIPK8 and PtrCIPK18 showed an overall downward trend under salt stress [ 37 ].…”

Section: Discussionsupporting

confidence: 82%

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Sequence Characteristics and Expression Analysis of GhCIPK23 Gene in Upland Cotton (Gossypium hirsutum L.)

Chao

Dong²,

Hu³

et al. 2022

IJMS

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CIPK (calcineurin B-like-interacting protein kinase) is a kind of serine/threonine protein kinase widely existing in plants, and it plays an important role in plant growth and development and stress response. To better understand the biological functions of the GhCIPK23 gene in upland cotton, the coding sequence (CDS) of the GhCIPK23 gene was cloned in upland cotton, and its protein sequence, evolutionary relationship, subcellular localization, expression pattern and cis-acting elements in the promoter region were analyzed. Our results showed that the full-length CDS of GhCIPK23 was 1368 bp, encoding a protein with 455 amino acids. The molecular weight and isoelectric point of this protein were 50.83 KDa and 8.94, respectively. The GhCIPK23 protein contained a conserved N-terminal protein kinase domain and C-terminal regulatory domain of the CIPK gene family member. Phylogenetic tree analysis demonstrated that GhCIPK23 had a close relationship with AtCIPK23, followed by OsCIPK23, and belonged to Group A with AtCIPK23 and OsCIPK23. The subcellular localization experiment indicated that GhCIPK23 was located in the plasma membrane. Tissue expression analysis showed that GhCIPK23 had the highest expression in petals, followed by sepals, and the lowest in fibers. Stress expression analysis showed that the expression of the GhCIPK23 gene was in response to drought, salt, low-temperature and exogenous abscisic acid (ABA) treatment, and had different expression patterns under different stress conditions. Further cis-acting elements analysis showed that the GhCIPK23 promoter region had cis-acting elements in response to abiotic stress, phytohormones and light. These results established a foundation for understanding the function of GhCIPK23 and breeding varieties with high-stress tolerance in cotton.

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Section: Discussionsupporting

confidence: 82%

“…The same results were observed in poplar and wheat [ 37 , 38 ]. In poplar, the gene expression of PtrCIPK1 , PtrCIPK5 , PtrCIPK8 and PtrCIPK18 showed an overall downward trend under salt stress [ 37 ]. Under high salinity conditions, TaCIPK25 expression was markedly downregulated in roots.…”

Section: Discussionsupporting

confidence: 82%

Sequence Characteristics and Expression Analysis of GhCIPK23 Gene in Upland Cotton (Gossypium hirsutum L.)

Chao

Dong²,

Hu³

et al. 2022

IJMS

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“…Under the long-term stresses, we observed the elevated expression of several genes related to Ca 2+ -dependent signaling and protein phosphorylation. Among them are CNX1 and CRT , whose products act as molecular chaperones ( Liu et al., 2017 ; Joshi et al., 2019 ); CIPK12 and CIPK6 products bind to CBLs—which are regulators of Ca 2+ -signal transduction ( Sardar et al., 2017 ; Czolpinska and Rurek, 2018 ; Guo et al 2018 ; Bai et al., 2022 ). Additionally, several genes related to Ca 2+ -signaling were upregulated (Strap, SAPK3 , PXL1 , INPP5A2 , GRP , etc.…”

Section: Discussionmentioning

confidence: 99%

Long-term cold, freezing and drought: overlapping and specific regulatory mechanisms and signal transduction in tea plant (Camellia sinensis (L.) Kuntze)

et al. 2023

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IntroductionLow temperatures and drought are two main environmental constraints reducing the yield and geographical distribution of horticultural crops worldwide. Understanding the genetic crosstalk between stress responses has potential importance for crop improvement.MethodsIn this study, Illumina RNA-seq and Pac-Bio genome resequencing were used to annotate genes and analyze transcriptome dynamics in tea plants under long-term cold, freezing, and drought.ResultsThe highest number of differentially expressed genes (DEGs) was identified under long-term cold (7,896) and freezing (7,915), with 3,532 and 3,780 upregulated genes, respectively. The lowest number of DEGs was observed under 3-day drought (47) and 9-day drought (220), with five and 112 genes upregulated, respectively. The recovery after the cold had 6.5 times greater DEG numbers as compared to the drought recovery. Only 17.9% of cold-induced genes were upregulated by drought. In total, 1,492 transcription factor genes related to 57 families were identified. However, only 20 transcription factor genes were commonly upregulated by cold, freezing, and drought. Among the 232 common upregulated DEGs, most were related to signal transduction, cell wall remodeling, and lipid metabolism. Co-expression analysis and network reconstruction showed 19 genes with the highest co-expression connectivity: seven genes are related to cell wall remodeling (GATL7, UXS4, PRP-F1, 4CL, UEL-1, UDP-Arap, and TBL32), four genes are related to calcium-signaling (PXL1, Strap, CRT, and CIPK6), three genes are related to photo-perception (GIL1, CHUP1, and DnaJ11), two genes are related to hormone signaling (TTL3 and GID1C-like), two genes are involved in ROS signaling (ERO1 and CXE11), and one gene is related to the phenylpropanoid pathway (GALT6).DiscussionBased on our results, several important overlapping mechanisms of long-term stress responses include cell wall remodeling through lignin biosynthesis, o-acetylation of polysaccharides, pectin biosynthesis and branching, and xyloglucan and arabinogalactan biosynthesis. This study provides new insight into long-term stress responses in woody crops, and a set of new target candidate genes were identified for molecular breeding aimed at tolerance to abiotic stresses.

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“…Under the long-term cold stress, we also observed the elevated expression of several genes, related to Ca 2+ -dependent signaling and protein phosphorylation in tea leaves. Among them, CNX1 (Calnexin) and calreticulin-like (CRT) which were reported to bind proteins on endoplasmic reticulum acting as molecular chaperones ( Liu et al., 2017 ; Joshi et al., 2019 ); also, CIPK12 and CIPK6 which were reported to bind CBLs regulating Ca 2+ -signal response ( Sardar et al., 2017 ; Czolpinska and Rurek, 2018 ; Bai et al., 2022 ). Additionally, several genes encoding the important components of membrane trafficking system and related to Ca 2+ -signaling were upregulated in tea plant under the long term cold stress, such as STRAP ( SERINE-THREONINE KINASE RECEPTOR-ASSOCIATED PROTEIN ) , SAPK3 ( SERINE/THREONINE-PROTEIN KINASE 3 ) , leucine-rich repeat receptor-like protein kinase PXY1 ( PHLOEM INTERCALATED WITH XYLEM-LIKE 1 ) , INPP5A2 type I inositol polyphosphate 5-phosphatase 2, glycine-rich protein A3-like GRP ( GLUTAMINE-RICH PROTEIN ), indicating their important roles in response to long-term cold stress in tea plant.…”

Section: Mechanisms Of Sensing and Signal Transduction Of Tea Plants ...mentioning

confidence: 99%

Recent progress and perspectives on physiological and molecular mechanisms underlying cold tolerance of tea plants

et al. 2023

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Tea is one of the most consumed and widely planted beverage plant worldwide, which contains many important economic, healthy, and cultural values. Low temperature inflicts serious damage to tea yields and quality. To cope with cold stress, tea plants have evolved a cascade of physiological and molecular mechanisms to rescue the metabolic disorders in plant cells caused by the cold stress; this includes physiological, biochemical changes and molecular regulation of genes and associated pathways. Understanding the physiological and molecular mechanisms underlying how tea plants perceive and respond to cold stress is of great significance to breed new varieties with improved quality and stress resistance. In this review, we summarized the putative cold signal sensors and molecular regulation of the CBF cascade pathway in cold acclimation. We also broadly reviewed the functions and potential regulation networks of 128 cold-responsive gene families of tea plants reported in the literature, including those particularly regulated by light, phytohormone, and glycometabolism. We discussed exogenous treatments, including ABA, MeJA, melatonin, GABA, spermidine and airborne nerolidol that have been reported as effective ways to improve cold resistance in tea plants. We also present perspectives and possible challenges for functional genomic studies on cold tolerance of tea plants in the future.

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Characterization of CBL-Interacting Protein Kinases’ Gene Family and Expression Pattern Reveal Their Important Roles in Response to Salt Stress in Poplar

Cited by 5 publications

References 35 publications

Sequence Characteristics and Expression Analysis of GhCIPK23 Gene in Upland Cotton (Gossypium hirsutum L.)

Sequence Characteristics and Expression Analysis of GhCIPK23 Gene in Upland Cotton (Gossypium hirsutum L.)

Long-term cold, freezing and drought: overlapping and specific regulatory mechanisms and signal transduction in tea plant (Camellia sinensis (L.) Kuntze)

Recent progress and perspectives on physiological and molecular mechanisms underlying cold tolerance of tea plants

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