Characterization of Cell Lines Developed From the Glassy-Winged Sharpshooter, Homalodisca Coagulata (Hemiptera: Cicadellidae)

Kamita, Shizuo G.; Zung, N.; Samra, Aman I.; Hagler, James R.; Hammock, Bruce D.

doi:10.1290/0501002.1

Cited by 13 publications

(2 citation statements)

References 25 publications

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“…Virions were found free or packed in crystalline arrays in large vesicles in the cytoplasm (58). Similar cytopathic effect (CPE) was also observed in RhPV-infected GWSS-Z10 cells (59), which are derived from the embryos of Homalodisca coagulate (60). Since the mitochondria and nuclear membranes remained intact during infection and the virus particles were not observed in the nuclei, these data imply that RhPV may replicate and assemble in the gut cells mitochondria.…”

Section: Infection Symptoms and Mechanisms Of Aphid Dicistrovirusessupporting

confidence: 56%

Aphid Viruses: A Brief View of a Long History

Guo

Bai

et al. 2022

Front. Insect Sci.

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Aphids are common agricultural pests with a wide range of hosts from agriculture to forestry plants. As known, aphids also serve as the major vectors to transmit plant viruses. Although numerous studies have focused on interactions between aphids and plant viruses, little is known about the aphid viruses, i.e., the insect viruses that are infectious to aphids. In the past four decades, several aphid viruses have been identified in diverse aphid species. In this review, we present a brief view of the aphid pathogenic viruses from several aspects, including classification of aphid viruses and characters of the viral genome, integration of viral sequences in host genomes, infection symptoms and influence on aphids, as well as host range and transmission modes. Taken together, these studies have increased our understanding of the rarely known aphid viruses, and will potentially contribute to the development of new strategies for controlling aphid populations.

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Section: Infection Symptoms and Mechanisms Of Aphid Dicistrovirusessupporting

confidence: 56%

Aphid Viruses: A Brief View of a Long History

Guo

Bai

et al. 2022

Front. Insect Sci.

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“…Specific facilities are required to rear live H. vitripennis in the United States; therefore, cell culture is more economical and a viable alternative, as well as increasingly vital for HoCV-01 detection and replication 2,9 . While basic methods for establishing cell cultures of H. vitripennis are described, these methods have not yet been utilized for commercial production of biological control agents, such as viruses 2 .…”

Section: Introductionmentioning

confidence: 99%

Propagation of <em>Homalodisca coagulata virus-01</em> via <em>Homalodisca vitripennis</em> Cell Culture

Biesbrock

Powell

Hunter

et al. 2014

JoVE

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The glassy-winged sharpshooter (Homalodisca vitripennis) is a highly vagile and polyphagous insect found throughout the southwestern United States. These insects are the predominant vectors of Xylella fastidiosa (X. fastidiosa), a xylem-limited bacterium that is the causal agent of Pierce's disease (PD) of grapevine. Pierce's disease is economically damaging; thus, H. vitripennis have become a target for pathogen management strategies. A dicistrovirus identified as Homalodisca coagulata virus-01 (HoCV-01) has been associated with an increased mortality in H. vitripennis populations. Because a host cell is required for HoCV-01 replication, cell culture provides a uniform environment for targeted replication that is logistically and economically valuable for biopesticide production. In this study, a system for large-scale propagation of H. vitripennis cells via tissue culture was developed, providing a viral replication mechanism. HoCV-01 was extracted from whole body insects and used to inoculate cultured H. vitripennis cells at varying levels. The culture medium was removed every 24 hr for 168 hr, RNA extracted and analyzed with qRT-PCR. Cells were stained with trypan blue and counted to quantify cell survivability using light microscopy. Whole virus particles were extracted up to 96 hr after infection, which was the time point determined to be before total cell culture collapse occurred. Cells were also subjected to fluorescent staining and viewed using confocal microscopy to investigate viral activity on F-actin attachment and nuclei integrity. The conclusion of this study is that H. vitripennis cells are capable of being cultured and used for mass production of HoCV-01 at a suitable level to allow production of a biopesticide. Video LinkThe video component of this article can be found at

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Insect cell culture and applications to research and pest management

Smagghe

Goodman

Stanley

2009

In Vitro Cell.Dev.Biol.-Animal

104

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Building on earlier research, insect cell culture began with the successful establishment of one cell line from pupal ovarian tissue. The field has grown to the extent that now over 500 insect cell lines have been established from many insect species representing numerous insect orders and from several different tissue sources. These cell lines are used as research tools in virology, in studies of signaling mechanisms to study insect immunity, hemocyte migration, and to test hypotheses about gene expression, and in screening programs designed to discover new insecticide chemistries. Virology research is revealing fundamentally new information on virus/host cell interactions. Studies in gene expression are uncovering signal transduction pathways that are new to insect science. Research is leading to the development of high-speed screening technologies that are essential in the search for new insect pest management tools. A few insect cell lines are, in routine industrial processes, designed to produce proteins of biomedical significance. Both primary cell cultures and established lines are used in basic biological studies to reveal how insect cells work. This review is designed to briefly cover the history of insect cell culture, recount some recent advances in the field, and offer a vision of the future of insect cell culture.

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Characterization of Cell Lines Developed From the Glassy-Winged Sharpshooter, Homalodisca Coagulata (Hemiptera: Cicadellidae)

Cited by 13 publications

References 25 publications

Aphid Viruses: A Brief View of a Long History

Aphid Viruses: A Brief View of a Long History

Propagation of <em>Homalodisca coagulata virus-01</em> via <em>Homalodisca vitripennis</em> Cell Culture

Insect cell culture and applications to research and pest management

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