Characterization of cells from pannus-like tissue over articular cartilage of advanced osteoarthritis

Yuan, Guohua; Tanaka, Mitsuya; Masuko, Kayo; Shibakawa, Akifumi; Kato, Tomohiro; Nishioka, Kusuki; Nakamura, Hiroshi

doi:10.1016/j.joca.2003.08.004

Cited by 54 publications

(56 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Formation of pannus tissue containing lymphocytes, macrophages, neutrophils, and fibroblasts and the growth of this tissue, which can invade and destroy the cartilage, could be stimulated in part by release of cytokines and chemokines by the chondrocytes in response to the initial matrix damage. Synovial inflammation and pannus formation have also been noted in patients with advanced OA (35,36), although usually not to the extent seen in RA. This is most likely due to a greater influx of cells into the joint driven by the early inflammatory activity in the RA synovium, while local generation of chemokines in OA cartilage would attract fewer cells to the joint due to the avascular nature of cartilage.…”

Section: Discussionmentioning

confidence: 99%

NF-κB Mediates the Stimulation of Cytokine and Chemokine Expression by Human Articular Chondrocytes in Response to Fibronectin Fragments

Pulai

Chen

et al. 2005

The Journal of Immunology

196

166

View full text Add to dashboard Cite

Fibronectin fragments (FN-f) that bind to the α5β1 integrin stimulate chondrocyte-mediated cartilage destruction and could play an important role in the progression of arthritis. The objective of this study was to identify potential cytokine mediators of cartilage inflammation and destruction induced by FN-f and to investigate the mechanism of their stimulation. Human articular chondrocytes, isolated from normal ankle cartilage obtained from tissue donors, were treated with a 110-kDa FN-f in serum-free culture, and expression of various cytokine genes was analyzed by cDNA microarray and by a cytokine protein array. Compared with untreated control cultures, stimulation by FN-f resulted in a >2-fold increase in IL-6, IL-8, MCP-1, and growth-related oncogene β (GRO-β). Constitutive and FN-f-inducible expression of GRO-α and GRO-γ were also noted by RT-PCR and confirmed by immunoblotting. Previous reports of IL-1β expression induced by FN-f were also confirmed, while TNF expression was found to be very low. Inhibitor studies revealed that FN-f-induced stimulation of chondrocyte chemokine expression was dependent on NF-κB activity, but independent of IL-1 autocrine signaling. The ability of FN-f to stimulate chondrocyte expression of multiple proinflammatory cytokines and chemokines suggests that damage to the cartilage matrix is capable of inducing a proinflammatory state responsible for further progressive matrix destruction, which also includes the chemoattraction of inflammatory cells. Targeting the signaling pathways activated by FN-f may be an effective means of inhibiting production of multiple mediators of cartilage destruction.

show abstract

Section: Discussionmentioning

confidence: 99%

NF-κB Mediates the Stimulation of Cytokine and Chemokine Expression by Human Articular Chondrocytes in Response to Fibronectin Fragments

Pulai

Chen

et al. 2005

The Journal of Immunology

196

166

View full text Add to dashboard Cite

show abstract

“…Yuan et al studied the characteristics of cells isolated from pannus-like soft tissue on osteoarthritic cartilage. He described that the osteoarthritis pannus-like cells shared the property of mesenchymal cells, chondrocytes, and the tissue involved in cartilage degeneration in osteoarthritis [20]. In 1996, the recruitment of mesenchymal cells from the synovial membrane into the partialthickness cartilage defect was reported by Hunziker E et al They described how their histological findings revealed a continuous layer of mesenchymal cells extending from the synovial membrane across the superficial tangential zone of normal articular cartilage into the defect, indicating that the cells that were recruited for the repair process were synovial in origin Study 1 and Study 2 shows the total mean score a At 2 and 4 weeks after surgery, Pineda's score showed no significant difference between cartilage defect with intact synovium group and cartilage defect without synovium group.…”

Section: Discussionmentioning

confidence: 99%

“…It was reported that foci and plaque formation of the pannus-like tissue over cartilage surface were found in an osteoarthritic knee, and the pannus-like tissue was found at the margins of articular surface or in more central areas [20]. However, whether the invading synovium forms cartilage-like tissue or degenerate cartilage has remained unclear [1,2,4,16].…”

Section: Introductionmentioning

confidence: 99%

The role of the synovium in repairing cartilage defects

Miyamoto

Deie

Yamasaki

et al. 2007

Knee Surg Sports Traumatol Arthr

View full text Add to dashboard Cite

The purpose of this study was to examine the role of the synovium in the transitional zone between the articular cartilage and the synovial membrane in cartilage repair and the relationship between the origin of the repaired cartilage and the grafted synovium. We used 8-week-old Sprague Dawley (SD) rats and green fluorescent protein (GFP) transgenic rats. In study 1, a full-thickness cartilage defect was created at the medial condyle of the femur, and the synovium 5 x 5 mm extending up to the cartilage defect was resected in the left knee (cartilage defect without synovium group) but not resected in the right knee (cartilage defect with intact synovium group). In study 2, after the creation of a full-thickness cartilage defect and resection of the synovium, the synovium of the GFP rats was transplanted into the unilateral knee (cartilage defect with transpl.synovium group). At 2, 4, 6, and 8 weeks after surgery, we evaluated the repaired tissue in cartilage defects histologically and immunohistochemically, and the expression of aggrecan and type II collagen in the repaired tissue was also investigated using reverse transcriptase-polymerase chain reactions (RT-PCR). At 6 and 8 weeks after surgery, the defect was filled with cartilage-like tissue in cartilage defect with intact synovium group and cartilage defect with transpl.synovium group, but not in cartilage defect without synovium group. GFP positive cells were observed in the repaired tissue and the expression of aggrecan and type II collagen was found in cartilage defect with transpl.synovium group. We concluded that the synovium in the transitional zone between the articular cartilage and the grafted synovial membrane invades the cartilage defects where the cells could be detected as GFP-positive cells. Those cells may take part in the repair and may induce chondrogenesis.

show abstract

“…MMP-1 and MMP-13 preferentially degrade type II collagen and may be most important in OA pathogenesis (Amalinei et al, 2007;Burrage et al, 2006). MMP-3 secreted by osteoarthritis synovial tissue was involved in direct extra-cellular matrix (ECM) breakdown (Yuan et al, 2004). ADAMTS-5 is the primary aggrecanase responsible for aggrecan degradation and ADAMTS-5 knockout mouse showed significantly reduced cartilage destruction (Glasson et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

WIN-34B, a new herbal medicine, inhibits the inflammatory response by inactivating IκB-α phosphorylation and mitogen activated protein kinase pathways in fibroblast-like synoviocytes

Huh¹,

Seo²,

Park³

et al. 2012

Journal of Ethnopharmacology

View full text Add to dashboard Cite

Characterization of cells from pannus-like tissue over articular cartilage of advanced osteoarthritis

Abstract: Our results showed that OA pannus cells shared the property of mesenchymal cells and chondrocytes; however, their origin seemed different from chondrocytes or synoviocytes. The spontaneous expression of MMPs suggests that they are involved in the articular degradation in OA.

Cited by 54 publications

References 27 publications

NF-κB Mediates the Stimulation of Cytokine and Chemokine Expression by Human Articular Chondrocytes in Response to Fibronectin Fragments

NF-κB Mediates the Stimulation of Cytokine and Chemokine Expression by Human Articular Chondrocytes in Response to Fibronectin Fragments

The role of the synovium in repairing cartilage defects

WIN-34B, a new herbal medicine, inhibits the inflammatory response by inactivating IκB-α phosphorylation and mitogen activated protein kinase pathways in fibroblast-like synoviocytes

Contact Info

Product

Resources

About