Characterization of Cellular Phenotypes and Cytokine Expression in Balt From Children With Congenital Heart Diseases

Moussallem, Tálib Moysés; Guedes, Fernanda; Fernandes, Elaine Aparecida; Pagliari, Carla; Andrade, Heitor Franco de; Duarte, Maria Irma Seixas; Lancellotti, Carmen Lúcia Penteado

doi:10.1080/pdp.22.6.449.459

Cited by 7 publications

(2 citation statements)

References 10 publications

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“…The paraffin-embedded lung biopsies were resectioned and 5 μ m sections were adhered to sylane-treated slides. Sections were submitted to immunohistochemistry with monoclonal antibodies as per the Streptavidin-Biotin Peroxidase method, using an endogenous biotin blocking system (DAKO, Carpinteria, CA) with modifications as described elsewhere (22,23). Positive and negative controls were used to avoid bias.…”

Section: Immunohistochemical Detection Of Cells Inflammation Phenotymentioning

confidence: 99%

In situ immune responses to interstitial pneumonitis in human visceral leishmaniasis

Tuon¹,

Guedes²,

Fernandes³

et al. 2009

Parasite Immunology

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Lung disease during active human visceral leishmaniasis is frequently reported. As such, studies have associated pulmonary symptoms to interstitial pneumonitis with a mononuclear infiltrate. However, the immune response in this condition has never been described before. The aim of this study was to determine the immunophenotypic pattern and cytokine profile of lung involvement (IPL) in human visceral leishmaniasis. Quantitative methods of analysis were performed using immunohistochemistry, and were compared with a control group of normal lung. Interstitial macrophages and cd8 cells were increased in IPL, and IL-4 as well as TNF-alpha displayed increased expression when compared to the control group. This inflammatory process with a Th2 pattern, as suggested by increased IL-4 and low IFN-gamma expression, is consistent with the immune response in other organs of visceral leishmaniasis. The microenvironment of the immune response in this condition is associated with lung disease in patients with interstitial pneumonitis related to visceral leishmaniasis, increasing the chance of bacterial infection.

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Section: Immunohistochemical Detection Of Cells Inflammation Phenotymentioning

confidence: 99%

In situ immune responses to interstitial pneumonitis in human visceral leishmaniasis

Tuon¹,

Guedes²,

Fernandes³

et al. 2009

Parasite Immunology

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“…The paraffin‐embedded mucosal biopsies were re‐sectioned and 5‐µm sections were adhered to sylane‐treated slides. The immunohistochemistry was performed with monoclonal antibodies and revealed by the Streptavidin‐Biotin Peroxidase method, using endogenous biotin blocking system (DAKO, USA) with modifications as elsewhere described (7,8). The following monoclonal antibodies for cytokines were used in this process: IL‐4 (AB204/R&D Systems, USA, MN), IL‐10 (MAB 217/R&D Systems, USA, MN), TNF‐α (IP300/Genzyme, UK), and IFN‐γ (IP 500/Genzyme, UK).…”

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confidence: 99%

Histopathology of mast cells and cytokines during healing of human mucosal leishmaniasis

Amato

Tuon

Nicodemo

et al. 2008

Parasite Immunology

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Mast cells (MCs) are associated with chronic inflammatory diseases. However, there is no study evaluating the importance of MCs in the mucosal leishmaniasis (ML). The aim of this study was to quantify the most important cytokines associated with mucosal leishmaniasis, before and after disease treatment, correlating with the healing. A cohort of 12 patients with ML was evaluated, and biopsies were taken before and after the treatment. A quantitative estimation of MCs and some cytokines was analysed by density of the labelled cells through immunohistochemistry. The MCs count in the tissue from patients with ML before treatment showed a mean of 29.3 +/- 37.9 cells/mm(2). The MCs count in patients with ML after healing decreased to 14.8 +/- 23.9 cells/mm(2). There was an inverse relation of MCs with IFN-gamma and IL-4 expression (r2 = 29.4 and r2 = 22.3 with P < 0.05). The expression of IL-10 and TNF-alpha was not related with MCs count. MCs decrease after treatment associated with decrease of IL-4 and IFN-gamma. The explanations of cytokine correlation are discussed in the article.

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TGF-beta and mesenchymal hepatic involvement after visceral leishmaniasis

et al. 2008

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The liver involvement in the human visceral leishmaniasis (VL) has been related to parasitism and activated Kupffer cells with further occasional fibrotic alterations, especially after long-term disease without treatment. However, fibrotic alterations have been reported after therapy, whose clinical finding is the persistence of hepatomegaly. Fibrotic involvement of the liver after therapy was never well understood, and the aim of this study was to evaluate this finding through ultrastructural and morphometric analysis. A case-control study was performed with 20 patients (15 cases and five controls). Cases included patients with persistent hepatomegaly (residual) after treatment of VL submitted to liver biopsy to exclude other causes of liver enlargement, including serum tests of viral hepatitis. The material was evaluated by electron microscopy allowing ultrastructural with morphometric analysis of medium portion of hepatic lobule. Narrow sinusoidal lumen and prominent Kupffer cells were found with insignificant alterations of hepatocytes, pit, and endothelial cells. On ultrastructural analysis, the enlargement of the space of Disse was due to fibrous collagen, increase of number of Ito cells, and nonfibrous extracellular matrix that were associated with Kupffer cells enlargement. Immunohistochemistry showed an intense expression of TGF-beta in patients with VL. These findings suggest a production of TGF-beta by Kupffer cells that resulted in the characteristic fibrotic involvement of the liver. Residual hepatomegaly in visceral leishmaniasis could result from sustained Kupffer cell activation with perihepatocytic fibrosis.

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Characterization of Cellular Phenotypes and Cytokine Expression in Balt From Children With Congenital Heart Diseases

Cited by 7 publications

References 10 publications

In situ immune responses to interstitial pneumonitis in human visceral leishmaniasis

In situ immune responses to interstitial pneumonitis in human visceral leishmaniasis

Histopathology of mast cells and cytokines during healing of human mucosal leishmaniasis

TGF-beta and mesenchymal hepatic involvement after visceral leishmaniasis

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