Characterization of Cesium Uptake Mediated by a Potassium Transport System of Bacteria in a Soil Conditioner

Abstract

We found that bacteria in a commercial soil conditioner sold in Ishinomaki, Miyagi, exhibited concentrative and saturable cesium ion (Cs ) uptake in the natural range of pH and temperature. The concentration of intracellular Cs could be condensed at least a few times higher compared with the outside medium of the cells. This uptake appeared to be mediated by a K transport system, since Cs uptake was dose-dependently inhibited by potassium ion (K ). Eadie-Hofstee plot analysis indicated that the Cs uptake involved a single saturable process. The maximum uptake amount (J max ) was the same in the presence and absence of K , suggesting that Cs and K uptakes were competitive with respect to each other. These bacteria might be useful for bioremediation of cesium-contaminated soil.

Bioremediation of Sr2+ ion radionuclide by using marine Streptomyces sp. CuOff24 extracellular polymeric substances

“…Later on, Tisakova et al reported the highest Cs + uptake by bacterium during 2 days of incubation period, as mature bacterial growth was attained within 1 day incubation time. 65 Similarly, Zhang et al 62 also observed an intracellular Cs + uptake which reached a maximum during 24 h incubation period. However, limited reports are available regarding the mechanism of biosorption, components and the pathway for the sorption, furthermore there is no exclusive study of biosorption of radionuclide ion by using EPS.…”

“…bacteria that could remove 80% of Sr from contaminated aquifer quartz sand by the soluble‐exchangeable fraction method. However, Zhang et al reported the Cs + sorbent experiment using soil associated bacteria and their characterization from near the Fukushima nuclear power plant accident site, Japan and those bacterial isolates could absorb only 10 μM CsCl. Compared to these observations, the EPS has distinctive and high biosorption capability and has the potential remediate efficiency to remove toxic substances.…”

“…Similarly, the Sr + resistant and urease producing bacterium of Halamonas sp., were recorded by Achal et al 15 and it could be adsorbed maximum Sr + ion at pH 7.5 after 4th days of incubation. Furthermore, Zhang et al 62 also studied the Cs + wileyonlinelibrary.com/jctb remediation in bacteria at pH 7.0 to 8.0 where the adsorption rate significantly decreased when the pH reached 8.5. 62 The temperature ranges from cold (10 ∘ C) to higher temperature (50 ∘ C) condition were used to study the adsorption of Sr 2+ by EPS.…”

“…Furthermore, Zhang et al 62 also studied the Cs + wileyonlinelibrary.com/jctb remediation in bacteria at pH 7.0 to 8.0 where the adsorption rate significantly decreased when the pH reached 8.5. 62 The temperature ranges from cold (10 ∘ C) to higher temperature (50 ∘ C) condition were used to study the adsorption of Sr 2+ by EPS. The Sr 2+ biosorption gradually increased while increasing the temperature level ( Fig.…”

Cs-131 as an experimental tool for the investigation and quantification of the radiotoxicity of intracellular Auger decays in vitro

“…The transport of K þ across the cell membrane or cell wall is vital for an organism's ability to maintain its membrane potential (Suhail 2010), and Cs-131 can be expected to be transported into other cell types or organisms, by a variety of K þ transporters. These cells/organisms include non-mammalian cells (Latorre and Miller 1983), bacteria (Zhang et al 2014), algae (Avery et al 1991(Avery et al , 1993 and fungi (Avery 1995).…”