Characterization of chemical constituents in Shuanghuanglian oral dosage forms by ultra‐high performance liquid chromatography coupled with time‐of‐flight mass spectrometry

Li, Ruiyun; Zhu, Yanru; Ma, Mingyan; Lei, Min; Zhao, Yingchun; Liu, Ting; Yu, Miao; Zhao, Yunli; Yu, Zhiguo

doi:10.1002/jssc.202100860

Cited by 17 publications

(14 citation statements)

References 30 publications

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“…We found 13 triterpenoids in Abrus mollis Hance by using the above criteria. According to the literature [28, 31, 32], during the cleavage process, these triterpenoids may lose some neutral molecules. For example, CO 2 and H 2 O or the oligosaccharide and monosaccharide fragment peaks can be lost, and the corresponding sugar unit fragment peaks will occur at the fractionation points.…”

Section: Resultsmentioning

confidence: 99%

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An ultra‐high performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry identification and characterization of the active constituents from Abrus mollis Hance

Tan

Chen

Zhang

et al. 2022

J of Separation Science

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Abrus mollis Hance is a traditional Chinese medicine that is widely used to treat acute and chronic hepatitis, steatosis, and fibrosis. Its therapeutic qualities of it have long been acknowledged, although the active ingredients responsible for its efficacy and the mechanisms of its action are unknown. In this study, the chemical constituents absorbed into the blood from Abrus mollis Hance were assessed by using liquid chromatography-quadrupole-time-of-flight mass spectrometry and the data was analyzed with the UNIFI screening platform. The results obtained were compared to existing chromatographic-mass spectrometry information, including retention times and molecular weights as well as known reference compounds. 41 chemical constituents were found in Abrus mollis Hance, and these included 16 flavonoids, 13 triterpenoids, five organic acids, and two alkaloids. Experimentally it was found that Abrus mollis Hance had a therapeutic benefit when treating α-naphthalene isothiocyanate-induced acute liver injury in rats. In addition, 11 blood prototypical constituents, including six flavonoids, three triterpenoids, and two alkaloids, were found in serum samples following intragastric administration of Abrus mollis Hance extracts to rats. This novel study can be used for the quality control and pharmacodynamic assessment of Abrus mollis Hance in order to assess its efficacy in the therapeutic treatment of patients.

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Section: Resultsmentioning

confidence: 99%

“…According to the cracking laws and the related literature [27][28][29], flavonoids are the key constituents of Abrus mollis Hance (Table 1). 16 flavonoids were found in this study and most of these were of either aglycones or O-glycosides types.…”

Section: Characterization and Identification Of Flavonoidsmentioning

confidence: 99%

An ultra‐high performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry identification and characterization of the active constituents from Abrus mollis Hance

Tan

Chen

Zhang

et al. 2022

J of Separation Science

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“…Studies such as those described by Li et al and Li et al provide theoretical support for consecutive research on the effective components and pharmacological experiments, and it is helpful for further development and usage. This facilitates the establishment of more equitable quality control standards [14,15].…”

Section: Chemical Composition Of the Spray-dried Extract Of L Albamentioning

confidence: 99%

Qualitative and quantitative chemical characterization of spray‐dried extracts and the development of an innovative oral solid formulation from Lippia alba

Almeida

Silva

Zimmermann

et al. 2023

Separation Science Plus

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A novel tablet formulation using Lippia alba was developed with spray‐dried extract leaf to obtain a product with anxiolytic properties. This work describes in silico study, extracting chemical qualitative and quantitative profiles, and the development and formulation of chemical‐physical characterization. A comprehensive understanding of L. alba oral dosage form components was obtained using a method based on high‐performance liquid chromatography coupled with a quadrupole time‐of‐flight mass spectrometer for the separation and characterization of L. alba tablets. Liquid chromatography‐tandem mass spectrometry analysis and Global Natural Product Social Molecular Networking allowed the identification of 36 compounds in the spray‐dried extract including flavonoids, iridoids, and phenylpropanoids glycosides. To determine biological markers, in silico analyzes were carried out with 15 compounds previously identified in this species. Acteoside was suggested as a biological marker, and the results of the Prediction of Activity Spectra for Substances pointed to a possible anxiolytic action. A quantitative method by high‐performance liquid chromatography‐diode array detection for Acteoside was also validated. The extract and formulation showed excellent flow characteristics, which favored the development of the formulation by direct compression. The tablets obtained were met for the quality control tests. Associating chemical strategies and technological development the novel tablet formulation could be used as a treatment for anxiolytic disorders.

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“…The chemical constituents of SHL are complex. Recently, 358 components have been identified in SHL oral liquid [1], and the primary chemical components were baicalin, chlorogenic acid, and forsythin [2]. SHL has been widely used in clinical practice in China to treat respiratory tract infections [3].…”

Section: Introductionmentioning

confidence: 99%

Qualitative and quantitative determination of the primary active components and metabolites in human plasma after oral administration of Shuanghuanglian liquid

Zhang

Liu

et al. 2022

J of Separation Science

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Shuanghuanglian oral liquid is a common traditional Chinese medicine used to treat respiratory tract infections. Its major components are baicalin, chlorogenic acid, and forsythin. In this study, the main drug‐related components in human plasma after oral administration of Shuanghuanglian were initially identified using ultra‐performance liquid chromatography‐ultraviolet detector/quadrupole time‐of‐flight mass spectrometry. Thirteen components from baicalin were identified, including the parent drug baicalin and aglycone baicalein. Only one metabolite related to chlorogenic acid, a sulfate conjugate formed after hydrolysis, and one metabolite related to forsythin, a sulfate conjugate of forsythin aglycone, were detected. Subsequently, a liquid chromatography‐tandem mass spectrometry method was established and validated to simultaneously determine baicalin and baicalein, the primary active components. After simple protein precipitation, the analytes were separated on a BEH C18 column using a 5 min‐gradient elution to avoid interference from baicalin isomers and their in‐source dissociation. Excellent linearity was observed over the concentration ranges of 5.00–2000 ng/ml for baicalin and 1.00–100 ng/ml for baicalein. The validated method was successfully applied to a pharmacokinetic study of an oral administration of 60 ml Shuanghuanglian in healthy subjects. This study provided a foundation to investigate the clinical efficacy and safety of Shuanghuanglian further.

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Characterization of chemical constituents in Shuanghuanglian oral dosage forms by ultra‐high performance liquid chromatography coupled with time‐of‐flight mass spectrometry

Cited by 17 publications

References 30 publications

An ultra‐high performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry identification and characterization of the active constituents from Abrus mollis Hance

An ultra‐high performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry identification and characterization of the active constituents from Abrus mollis Hance

Qualitative and quantitative chemical characterization of spray‐dried extracts and the development of an innovative oral solid formulation from Lippia alba

Qualitative and quantitative determination of the primary active components and metabolites in human plasma after oral administration of Shuanghuanglian liquid

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