Characterization of chicken interleukin 2 receptor α chain, a homolog to mammalian CD25

Teng, Quincy; Zhou, Jiyong; Wu, Jianming; Guo, Junqing; Shen, Huigang

doi:10.1016/j.febslet.2006.06.044

Cited by 40 publications

(23 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CD25 + is a T cell activation marker in chickens. Conventional T cells transiently upregulate CD25 + in the periphery during inflammation in chickens (Hála et al, 1986;Kim et al, 2000;Teng et al, 2006). CD25 + is also a Treg-specific marker.…”

Section: Discussionmentioning

confidence: 98%

Intestinal physiology and regulatory T cell response to immediate or delayed access to feed and water in Pekin ducklings,

Loudon

Shanmugasundaram²,

Lilburn³

et al. 2011

Poultry Science

View full text Add to dashboard Cite

This study investigated the effects of delayed access to feed and water on early duckling growth, selected aspects of intestinal physiology, and immune responses. Ducklings were assigned to one of 2 experimental groups. In one treatment group (referred to as the fed group), feed and water were provided beginning on d 0, and in the other treatment group (referred to as the withheld group), feed and water were withheld until d 2. The ducklings in the withheld treatment had lower BW at 2 and 6 d posthatch than ducklings in the fed group. At 8 d posthatch (6 d postfeeding), the BW of the ducklings in the withheld group was comparable to the BW of the 6-d-old ducklings in the fed group. At 2 d posthatch, withheld ducklings had lower absolute and relative duodenum plus pancreas weight than fed ducklings. At 8 d posthatch (6 d postfeeding), withheld ducklings had higher absolute and relative duodenum plus pancreas weight than fed ducklings at 6 d posthatch. At 2 d posthatch, mucin 5B mRNA content was approximately 2 times lower in withheld ducklings (P = 0.09) than in fed ducklings. At 6 d posthatch, mucin 5b mRNA content was approximately 2.5-fold higher in withheld ducklings (P = 0.07) than in fed ducklings. Delayed access to feed and water increased the CD25(+) cell number in the cecal tonsil at 2, 6, and 8 d posthatch. The IL-10 content of CD25(+) cells was higher in the withheld ducklings than in the fed ducklings at 2 and 6 d posthatch. In conclusion, delaying access of ducklings with no experimental pathogen infection to feed and water has no long-term effects on early growth parameters, intestinal physiology, and immune responses.

show abstract

Section: Discussionmentioning

confidence: 98%

Intestinal physiology and regulatory T cell response to immediate or delayed access to feed and water in Pekin ducklings,

Loudon

Shanmugasundaram²,

Lilburn³

et al. 2011

Poultry Science

View full text Add to dashboard Cite

show abstract

“…In mammals, TCR-stimulated T cells upregulate CD25 in the presence of IL-2. In chicken, CD25 is an activation marker (24), and CD25 are (23). Anti-chicken CD25 mAb stained a higher percentage of cells in Con Atreated groups compared with the naive cells (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…CD25-His tag fusion protein was purified using a nickel column under denaturing conditions following the manufacturer's instructions (Qiagen). CD25-His tag fusion protein was analyzed by SDS-PAGE and Western blot using anti-His tag mAb (Qiagen) (23). Mouse anti-chicken CD25 was produced by Promab Biotechnologies (Richmond, CA).…”

Section: Mouse Anti-chicken Cd25 Mabmentioning

confidence: 99%

“…CD25, an IL-2R, is expressed in chicken monocytes, macrophages, thrombocytes, CD4 + cells, and CD8 + cells (23) and is a marker of T cell activation in chickens (24). Though CD25 + cells have been identified in chicken, the CD4 + CD25 + subpopulation has not been isolated and characterized for classical mammalian Treg properties such as proliferation of T cell suppression assay or suppressive cytokine production.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Regulatory T Cell Properties of Chicken CD4+CD25+ Cells

Shanmugasundaram

Selvaraj

2011

The Journal of Immunology

116

View full text Add to dashboard Cite

Chicken CD4+CD25+ cells were characterized for mammalian regulatory T cells’ suppressive and cytokine production properties. Anti-chicken CD25 mAb was produced in mice and conjugated with a fluorescent tag. The specificity of the Ab against chicken CD25 was confirmed by evaluating Con A-induced CD25 upregulation in thymocytes and by quantifying the CD25 mRNA content of positive and negative cells identified by anti-chicken CD25 Ab. The percentage of CD4+CD25+ cells, expressed as a percentage of CD4+ cells, in thymus and blood was ∼3–7%, in spleen was 10%, and in cecal tonsil, lung, and bone marrow was ∼15%. Bursa had no detectable CD4+CD25+ cells. CD25+ cells were mostly CD4+ in the thymus, whereas in every other organ studied, CD25+ cells were distributed between CD4+ and CD4− cells. Chicken thymic CD4+CD25+ cells did not proliferate in vitro in the absence of recombinant chicken IL-2 (rCIL-2). In the presence of rCIL-2, PMA plus ionomycin or Con A stimulated CD4+CD25+ cell proliferation, whereas anti-CD3 plus CD28 did not stimulate CD4+CD25+ cell proliferation. Naive CD4+CD25+ cells had 29-fold more IL-10 mRNA and 15-fold more TGF-β mRNA than the naive CD4+CD25− cells. Naive CD4+CD25+ had no detectable IL-2 mRNA. Both naive and PMA plus ionomycin-stimulated thymic CD4+CD25+ cells suppressed naive T cell proliferation. The suppressive properties were partially contact dependent. Supplementing CD4+CD25+ cell coculture with rCIL-2 reversed the suppressive properties of CD4+CD25+ cells. Chicken CD4+CD25+ cells have suppressive properties similar to that of mammalian regulatory T cells.

show abstract

“…+ cells found in our study may include a range of lymphocyte subsets with distinct activities, especially because CD25 is part of the IL-2 receptor (Teng et al, 2006). Moreover, splenic memory T cells constitutively express the CD25 marker (Reinhardt et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Dexamethasone Regulates Macrophage and Cd4+Cd25+ Cell Numbers in the Chicken Spleen

Calefi

Quinteiro-Filho

Fukushima

et al. 2016

Rev. Bras. Cienc. Avic.

View full text Add to dashboard Cite

Dexamethasone (DEX) is a corticoid hormone that is experimentally used to mimic the effects of increased levels of endogenous corticosterone observed during the stress response. Currently, stress is considered one of the major predisposing factors for diseases in the poultry industry. The aim of this study was to analyze the effects of DEX and/or of a 20-fold coccidial vaccine dose on leukocyte phenotypes in the spleen and cecal tonsils of chickens. Twenty specific-pathogen-free (SPF) Leghorn chickens were divided into four groups: a non-treated group (NT), a DEX-treated group (Dex), a vaccinated group (V) and a DEX-treated+vaccinated group (Dex+V). On experimental day (ED) 42, each bird in the vaccinated groups received a anti-coccidial vaccine. DEX was injected in the birds of the Dex and Dex+V groups (0.9 mg/kg) onED42 and ED45. + cells in the spleen of birds of the V group was higher than those measured in the other experimental groups. Our data suggest that CD4 + CD25 + cells and macrophages might be influenced by DEX treatment in spleen, but not in the cecal tonsils of chickens inoculated with Eimeria.

show abstract

Characterization of chicken interleukin 2 receptor α chain, a homolog to mammalian CD25

Cited by 40 publications

References 52 publications

Intestinal physiology and regulatory T cell response to immediate or delayed access to feed and water in Pekin ducklings,

Intestinal physiology and regulatory T cell response to immediate or delayed access to feed and water in Pekin ducklings,

Regulatory T Cell Properties of Chicken CD4+CD25+ Cells

Dexamethasone Regulates Macrophage and Cd4+Cd25+ Cell Numbers in the Chicken Spleen

Contact Info

Product

Resources

About