Characterization of chlorobenzoate degraders isolated from polychlorinated biphenyl-contaminated soil and sediment in the Czech Republic

Abstract

Two polychlorinated biphenyl‐contaminated sites in the Czech Republic, a soil at Zamberk and a sediment sludge at Milevsko, were screened for the presence of chlorobenzoate degraders. Sixteen different chlorobenzoate degraders were isolated from the soil compared with only three strains isolated from the sediment. From these strains, only four soil degraders and one strain isolated from the sediment, respectively, were shown to possess a complete chlorobenzoate (CB) pathway. Bacteria isolated from the soil have expressed more flexibility for CB degradation, namely in the case of ortho‐chlorinated benzoates. They all possessed large plasmids, the restriction patterns of which were compared. Plasmids in Pseudomonas sp. A7, A8, A18 and A19, respectively, were cured and found to encode at least part of the metabolic pathway involved in the growth on ortho‐chlorinated benzoates.

Integration of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Cloning for the Identification and Functional Characterization of Mobile ortho -Halobenzoate Oxygenase Genes in Pseudomonas aeruginosa Strain JB2

“…While several strains have been reported to utilize 2-chlorobenzoate (2-CBa) as a growth-supporting substrate (13,15,17,27,37,42,47,50), organisms with this phenotype appear to be isolated less frequently than organisms that are able to utilize 3-or 4-CBa. The molecular genetics and physiology of 2-CBa degradation have been studied in detail in two organisms, Burkholderia cepacia strain 2-CBS and Pseudomonas aeruginosa strain 142.…”

Analysis of a Bacterial Community in 3-Chlorobenzoate-Contaminated Soil by PCR-DGGE Targeting the 16S rRNA Gene and Benzoate 1,2-Dioxygenase Gene (benA)

“…To date, various bacteria that can degrade CBs have been isolated from different environmental samples 3,[6][7][8]15,25,29) . It is not clear, however, which bacterial groups are the main contributors to the degradation of CBs under environmental conditions, because most of the degraders are obtained by screening based on their ability to degrade CBs on agar plates or in liquid cultures.…”

General and molecular microbiology and microbial genetics in the IM CAS

“…These vectors were used for analysis of catRABC operon involved in catechol degradation in phenol-degrading Rhodococcus erythropolis [221]. The bacterial chlorobenzoate degraders isolated form contaminated soils in the Czech Republic were also characterized [13,176]. Metagenomic approaches revealed abundance of meta-cleavage pathways of aromatic degradation in microbial communities from soil highly contaminated with jet fuel [14].…”