Characterization of cholecystokinin receptors in canine intestinal circular muscle

Mao, Yu; Wang, Y.F.; Daniel, E. E.

doi:10.1016/0196-9781(95)00072-r

Cited by 4 publications

(1 citation statement)

References 13 publications

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“…Previously, the affinities of L‐365,260, L‐364,718 and YM022 have been investigated in radioligand binding studies conducted on canine small intestine circular muscle using L‐364,718 as radioligand (Mao et al ., 1995). Consistent with our results, Mao and co‐workers demonstrated that L‐364,718 expressed a higher affinity than L‐365,260 at putative CCK1 receptor binding sites labelled with [ 3 H]L‐364,718; however, they reported no inhibitory effect of YM022 at the same binding sites expressed in the canine intestine.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning, expression and pharmacological characterization of the canine cholecystokinin 1 receptor

Morton

Pyati

Dai

et al. 2005

British J Pharmacology

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1 The full-length, canine cholecystokinin 1 (CCK1) receptor was cloned from gallbladder tissue using RT-PCR with a combination of primers designed to interact with conserved regions of the human and rat CCK1 receptor, which also shared homology with the canine genomic sequence. . Furthermore, the selectivity of these compounds between canine CCK1 and CCK2 receptors was consistent with the selectivity between the human CCK1 and CCK2 receptors. 4 Two additional forms of the canine CCK1 receptor were identified during the cloning procedure. These had three (variant #1) and six (variant #2) amino-acid differences from the wild-type canine CCK1 receptor. Variant #1 bound [ 125 I]BH-CCK-8S and displayed an identical pharmacological profile to the wild-type receptor using the ligands described above. No significant binding was measured with variant #2. 5 In conclusion, we have cloned and pharmacologically characterized the canine CCK1 receptor. The data obtained will facilitate the interpretation of numerous pharmacological experiments that have been performed using canine tissue to elucidate the actions of CCK and gastrin.

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Section: Discussionmentioning

confidence: 99%