Characterization of chondroitin sulfate in stem cells derived from umbilical cord blood in rats

Abstract

Chondroitin sulfate (CS) and its isomeric variant, dermatan sulfate (DS), are complex glycosaminoglycans (GAGs) which are ubiquitous components of the extracellular matrix in various tissues including the brain. CS and/or DS are known to bind to a variety of growth factors and regulate many cellular events such as proliferation and differentiation. Although the biological activities of CS and/or DS towards neural stem/progenitor cells (NSPCs) have been well investigated, the CS and/or DS of hematopoietic stem cells (HSCs) have not been fully characterized. Here, we analyzed GAGs on mononuclear cells of rat umbilical cord blood cells (UCB-MNCs). CS was detected in vascular intima and media of rat umbilical cord at embryonic day 19 (E19) by immunohistochemistry. The stem-cell-enriched-UCBCs (SCE-UCBCs), which were expanded from rat UCB-MNCs, expressed CS. CS chains are composed of repeating disaccharide units, which are classified into several types such as O-, A-, B-, C-, D-, and E-unit according to the number and positions of sulfation. A disaccharide composition analysis revealed that CS and/or DS were abundant in rat UCB-MNCs as well as in their expanded SCE-UCBCs, while the amount of heparan sulfate (HS) was less. The degree of sulfation of CS/DS was relatively low and the major component in UCB-MNCs and SCE-UCBCs was the A-unit. A colony-forming cell assay revealed that the percentage of colony-forming cells decreased in culture with CS degradation enzyme. The CS and/or DS of UCBCs may be involved in biological activities such as stem cell proliferation and/or differentiation.

Pregnant Women' Knowledge and Attitudes toward Cord Blood Stem Cell Banking and Donation at Gharbia Governorate, Egypt

“…It was observed that, the majority (88.4%) of the studied women did not attend any health teaching classes related to blood cord stem cell banking and donation. Figure (2): reveals levels of Knowledge regarding blood cord and stem cells among the studied pregnant women. It was observed that more than two third (67.0%) of the studied pregnant women had low level of knowledge regarding blood cord and stem cells and only 11.0% of them had high level of knowledge regarding blood cord and stem cells.…”

“…The components of cord blood are the same as those found in other blood that can be found in any organ. It is unique, though, because it has an excess of hematopoietic cells (2,3) Hematopoietic stem cells (HSCs) are multi potent primitive cells that can develop into all types of blood cells. HSCs can form mature blood cells, such as red blood cells, platelets, and white blood cells (4).…”

Glycan Modifications as Regulators of Stem Cell Fate

“…The sulfation arrangement within HS chains profoundly influences the specificity of interaction [39,40]. Chondroitin sulfate (CS) and its isomeric variant, dermatan sulfate (DS), are major components of the extracellular matrix in various tissues [41]. CS and DS are known to serve as ligands for a variety of growth factors and regulate many cellular events including morphogenesis, proliferation, and differentiation [42].…”

Spatiotemporal diversity and regulation of glycosaminoglycans in cell homeostasis and human disease

“…Furthermore, multiple diverse forms of CS/DS (CS A–E) with distinct sulfated disaccharide repeats are present across various species and tissues. Depending on sulfation pattern and IdoA content, CS/DS chains can be classified into different subtypes: CS-A [GlcA-GalNAc(4S)], CS-B/DS [IdoA(2S)-GalNAc(4S)], CS-C [GlcA-GalNAc(6S)], CS-D [GlcA(2S)-GalNAc(6S)], CS-E [GlcA-GalNAc(4S,6S)], and CS-iE [IdoA-GalNAc(4S,6S)] ( 77 , 78 ). Interestingly, these different types of CS exhibit distinct spatiotemporal expression across tissues, particularly in the brain ( 79 , 80 ).…”