Characterization of Citrus aurantifolia bioactive compounds and their inhibition of human pancreatic cancer cells through apoptosis

Patil, Bhimanagouda S.; Jayaprakasha, G.K.; Murthy, Kotamballi N. Chidambara; Chetti, M. B.; Patil, Bhimanagouda S.

doi:10.1016/j.microc.2009.09.008

Cited by 58 publications

(38 citation statements)

References 42 publications

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“…The methanolic extract of this plant exhibited the maximum activity with an IC 50 value of 81.20 µg/mL after 72 h. The reported inhibition of Panc-28 cells was in the range of 73%-89%, at 100 µg/mL after 96 h. The involvement of apoptosis in induction of cytotoxicity was further confirmed by expression of Bax, Bcl-2, caspase-3, and p53 [48]. Similarly, Patil et al [52] studied the proliferation inhibitory activity in human pancreatic (Panc-28) cell lines by 1, limonin glucoside (11), 5 and 6 isolated from C. aurantifolia. The IC 50 [73].…”

Section: In Vitro Testsmentioning

confidence: 59%

“…Their amounts were found to be higher than those found in other citrus fruits [168]. The isolation and identification of both limonoids aglycones and glucosides by routinary HPLC and spectrometric or spectroscopic analyses is reported in several recent papers [48,52,169].…”

Section: Limonoid Aglycones and Glucosidesmentioning

confidence: 92%

“…The unbalanced solubility profile of the latter may explain the relatively low number of studies on their respective biological activities which are hampered by difficult isolation from natural sources and poor pharmacokinetics. Analgesic [45], anticancer [46][47][48][49][50][51][52][53][54][55][56][57][58][59], anti-inflammatory [45,[60][61][62], antibacterial [63,64], antioxidant [4,65,66], antiviral [67], larvicidal [68,69] Anticancer [46,47,58,59,70], anti-hyperglycemic [71], anti-inflammatory [25,62], antioxidant [4,65,66], antiviral [67], larvicidal [68,69] …”

Section: Chemistry and Classificationsmentioning

confidence: 99%

“…Only two studies reported the isolation of limonexic acid (5) in the last decade (two further studies reporting on the isolation of both 5 and its β-D-glucoside are treated in Section 3.3 [48,52]. After isolation from C. aurantium var.…”

Section: Limonoid Aglyconesmentioning

confidence: 99%

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The Chemistry and Pharmacology of Citrus Limonoids

et al. 2016

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Citrus limonoids (CLs) are a group of highly oxygenated terpenoid secondary metabolites found mostly in the seeds, fruits and peel tissues of citrus fruits such as lemons, limes, oranges, pumellos, grapefruits, bergamots, and mandarins. Represented by limonin, the aglycones and glycosides of CLs have shown to display numerous pharmacological activities including anticancer, antimicrobial, antioxidant, antidiabetic and insecticidal among others. In this review, the chemistry and pharmacology of CLs are systematically scrutinised through the use of medicinal chemistry tools and structure-activity relationship approach. Synthetic derivatives and other structurally-related limonoids from other sources are include in the analysis. With the focus on literature in the past decade, the chemical classification of CLs, their physico-chemical properties as drugs, their biosynthesis and enzymatic modifications, possible ways of enhancing their biological activities through structural modifications, their ligand efficiency metrics and systematic graphical radar plot analysis to assess their developability as drugs are among those discussed in detail.

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Section: In Vitro Testsmentioning

confidence: 59%

Section: Limonoid Aglycones and Glucosidesmentioning

confidence: 92%

Section: Chemistry and Classificationsmentioning

confidence: 99%

Section: Limonoid Aglyconesmentioning

confidence: 99%

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The Chemistry and Pharmacology of Citrus Limonoids

et al. 2016

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“…The studies in vitro and in vivo indicated that limonin is a potential bioactive compound which has many properties for promoting human health, such as lowering cholesterol, anticancer, antiviral and a number of other pharmacological activities [3][4][5][6][7]. The identification of limonin has also been reported [4,[8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Extraction of Limonin from Orange (<i>Citrus reticulata</i> Blanco) Seeds by the Flash Extraction Method

Liu

Rong

et al. 2012

SERDJ

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A flash extraction method was investigated for mass production of limonin from orange (Citrus reticulata Blanco) seeds. The limonin was extracted by using flash extraction for 2 min. The extraction conditions optimized by response surface methodology were as follows: ethanol concentration, 72% (v/v); solvent/solid ratio, 29:1 mL/g; rotational speed, 4000 r/min. The limonin was crystallized from the mixed solution of dichloromethane and isopropanol (1:3) at 4 o C for 1 h. The limonin crystals were identified by high performance liquid chromatography (HPLC) and from their infrared spectrum (IR). The purity of limonin was 95%, the yield of limonin was 6.8 mg/g and the recovery yield of limonin was 97.1%. Thus, flash extraction is an efficient method for the mass production of limonin.

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