Characterization of clinically relevant model bacterial strains of Pseudomonas aeruginosa for anti-biofilm testing of materials

Rzhepishevska, Olena; Ліманська, Н. В.; Галкін, М. Б.; Lacoma, Alícia; Lundquist, Margaretha; Sokol, Dmytro; Hakobyan, Shoghik; Sjöstedt, Anders; Prat, Cristina; Ramstedt, Madeleine

doi:10.1016/j.actbio.2018.06.019

Cited by 10 publications

(8 citation statements)

References 33 publications

(71 reference statements)

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“…The impact of surface chemistry on infection and biofilms has been studied predominantly from a biomaterial perspective, with the aim of developing resistant coatings. Surfaces that allow bacterial adhesion are found to support infection, and therefore, surface chemistries that avoid cell capture or retention are sought. , There has been limited effort to quantitatively distinguish characteristics of biofilms on surfaces of different bacterial-adhesive chemistries, − and we are far from a general understanding. Studies have determined that adhesive interactions can originate with pili − and flagella, − and these appendages further influence the motions of adhered bacteria such as twitching and rotating. , Adhesive interactions involving the cell body with a surface are thought, at least in some cases, to be stronger than the adhesion via appendages .…”

Section: Introductionmentioning

confidence: 99%

Surface Chemistry Guides the Orientations of Adhering E. coli Cells Captured from Flow

Niu

Rivera

et al. 2021

Langmuir

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Motivated by observations of cell orientation at biofilm−substrate interfaces and reports that cell orientation and adhesion play important roles in biofilm evolution and function, we investigated the influence of surface chemistry on the orientation of Escherichia coli cells captured from flow onto surfaces that were cationic, hydrophobic, or anionic. We characterized the initial orientations of nonmotile cells captured from gentle shear relative to the surface and flow directions. The broad distribution of captured cell orientations observed on cationic surfaces suggests that rapid electrostatic attractions of cells to oppositely charged surfaces preserve the instantaneous orientations of cells as they rotate in the near-surface shearing flow. By contrast, on hydrophobic and anionic surfaces, cells were oriented slightly more in the plane of the surface and in the flow direction compared with that on the cationic surface. This suggests slower development of adhesion at hydrophobic and anionic surfaces, allowing cells to tip toward the surface as they adhere. Once cells were captured, the flow was increased by 20-fold. Cells did not reorient substantially on the cationic surface, suggesting a strong cell−surface bonding. By contrast, on hydrophobic and anionic surfaces, increased shear forced cells to tip toward the surface and align in the flow direction, a process that was reversible upon reducing the shear. These findings suggest mechanisms by which surface chemistry may play a role in the evolving structure and function of microbial communities.

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Section: Introductionmentioning

confidence: 99%

Surface Chemistry Guides the Orientations of Adhering E. coli Cells Captured from Flow

Niu

Rivera

et al. 2021

Langmuir

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“…Finally, we used the laboratory adapted Pa strain, PAO1. There is large variation in the phenotype of clinical strains of Pa, including biofilm formation, LPS structure and the release of virulence factors and also Pili, flagella mutants [89][90][91]. Previously, a difference in the signalling cascade induced by mucoid and non-mucoid strains of Pa has been seen, with mucoid strains inducing corticosteroid-resistant inflammation, driven by TLR2 signalling [92].…”

Section: Plos Onementioning

confidence: 99%

Pseudomonas aeruginosa induces p38MAP kinase-dependent IL-6 and CXCL8 release from bronchial epithelial cells via a Syk kinase pathway

et al. 2021

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Pseudomonas aeruginosa (Pa) infection is a major cause of airway inflammation in immunocompromised and cystic fibrosis (CF) patients. Mitogen-activated protein (MAP) and tyrosine kinases are integral to inflammatory responses and are therefore potential targets for novel anti-inflammatory therapies. We have determined the involvement of specific kinases in Pa-induced inflammation. The effects of kinase inhibitors against p38MAPK, MEK 1/2, JNK 1/2, Syk or c-Src, a combination of a p38MAPK with Syk inhibitor, or a novel narrow spectrum kinase inhibitor (NSKI), were evaluated against the release of the proinflammatory cytokine/chemokine, IL-6 and CXCL8 from BEAS-2B and CFBE41o- epithelial cells by Pa. Effects of a Syk inhibitor against phosphorylation of the MAPKs were also evaluated. IL-6 and CXCL8 release by Pa were significantly inhibited by p38MAPK and Syk inhibitors (p<0.05). Phosphorylation of HSP27, but not ERK or JNK, was significantly inhibited by Syk kinase inhibition. A combination of p38MAPK and Syk inhibitors showed synergy against IL-6 and CXCL8 induction and an NSKI completely inhibited IL-6 and CXCL8 at low concentrations. Pa-induced inflammation is dependent on p38MAPK primarily, and Syk partially, which is upstream of p38MAPK. The NSKI suggests that inhibiting specific combinations of kinases is a potent potential therapy for Pa-induced inflammation.

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“…However, the results obtained with these systems cannot be completely translated into natural environments as the model strains were not isolated at the same time, nor at the place where the material is expected to work [91]. Indeed, as these lab strains are normally kept in laboratory stocks and have been cultured routinely, they may not exhibit the same phenotype as fresh isolates [92].…”

Section: Microbial Choicementioning

confidence: 99%

“…Another question is how to select the most relevant microorganisms among other isolates. At the moment, no consensus exists in the field, making results very difficult to compare between different works [92]. In the study of Rzhepishevska et al [92], 19 strains of P. aeruginosa originating from hospitalized patients were studied and compared to the lab reference strain PAO1 and a rmlC lipopolysaccharide PAO1 mutant.…”

Section: Microbial Choicementioning

confidence: 99%

“…At the moment, no consensus exists in the field, making results very difficult to compare between different works [92]. In the study of Rzhepishevska et al [92], 19 strains of P. aeruginosa originating from hospitalized patients were studied and compared to the lab reference strain PAO1 and a rmlC lipopolysaccharide PAO1 mutant. The authors observed two sets of isolates, a group with high adhesion to a polymeric anti-biofilm coating and a group with low adhesion, including PAO1.…”

Section: Microbial Choicementioning

confidence: 99%

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Testing Anti-Biofilm Polymeric Surfaces: Where to Start?

Cattò

Cappitelli

2019

IJMS

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Present day awareness of biofilm colonization on polymeric surfaces has prompted the scientific community to develop an ever-increasing number of new materials with anti-biofilm features. However, compared to the large amount of work put into discovering potent biofilm inhibitors, only a small number of papers deal with their validation, a critical step in the translation of research into practical applications. This is due to the lack of standardized testing methods and/or of well-controlled in vivo studies that show biofilm prevention on polymeric surfaces; furthermore, there has been little correlation with the reduced incidence of material deterioration. Here an overview of the most common methods for studying biofilms and for testing the anti-biofilm properties of new surfaces is provided.

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Characterization of clinically relevant model bacterial strains of Pseudomonas aeruginosa for anti-biofilm testing of materials

Cited by 10 publications

References 33 publications

Surface Chemistry Guides the Orientations of Adhering E. coli Cells Captured from Flow

Surface Chemistry Guides the Orientations of Adhering E. coli Cells Captured from Flow

Pseudomonas aeruginosa induces p38MAP kinase-dependent IL-6 and CXCL8 release from bronchial epithelial cells via a Syk kinase pathway

Testing Anti-Biofilm Polymeric Surfaces: Where to Start?

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