Characterization of combinatorially designed polyarylates by time-of-flight secondary ion mass spectrometry

Abstract

A series of 16 polyarylates, with well-controlled and systematically varying chemistry, has been characterized by time-of-flight secondary ion mass spectrometry (TOF-SIMS). The polymers are structurally identical except for the incremental additions of C2H4 units to the backbone and sidechain. From the spectra, peaks characteristic of all polyarylates are identified. Furthermore, evaluation of the spectra and identification of unique signals allow classification of the polyarylates according to sidechain and backbone chemistry.

Cellular response to phase‐separated blends of tyrosine‐derived polycarbonates

“… Negative‐ion TOF‐SIMS of the tyrosine‐derived homopolymers and the respective blends showing the characteristic peaks for DTE (191) and DTO (275) and the chemical structures for the common ion fragments first identified by Belu et al36 show that both materials are present at the surface in the phase‐separated blends. …”

“…To evaluate the responses of macrophages and osteoblasts to DTE, DTO, and the respective blends, cells of each type were plated on polymer-coated disks, and mRNA was harvested from the samples after 24 h of incubation. The studies described herein measure how the genetic expression levels of IL-1␤ and FN vary for the series of blends of DTE and DTO in two different 36 show that both materials are present at the surface in the phase-separated blends. cell lines.…”

“…The possible presence of a hydrophobic wetting layer due to the water contact angle being very close to that of the DTO homopolymers led to the use of time-of-flight secondary-ion mass spectrometry (TOF-SIMS), which was able to measure the relative amounts of each polymer present at the surface. The SIMS technique used in these analyses is sensitive to the top 10 Å of the material surface, where both of the characteristic peaks of the DTE and DTO polymers identified previously by Belu et al 36 have been measured. The characteristic fragmentation peaks for DTE (191) and DTO (275) are noted in the respective spectra of Figure 3.…”

Biomaterials Informatics

“…Time-of-fl ight secondary-ion mass spectrometry (TOF-SIMS) can be used to identify the polymer repeat units on the surface of the material, which may give specifi c information about the orientation of block copolymer morphology to the surface (i.e., layered microstructure parallel or orthogonal to the substrate), or simply distinguish a particular polymer from a family of structurally related materials [38]. Characterization of polyarylates using this technique resulted in the identifi cation of fi ngerprints for both the backbone (or diacid component) and the length of the ester sidechain of the diol component.…”

Integration of Combinatorial Synthesis, Rapid Screening, and Computational Modeling in Biomaterials Development

“…To analyze the more than 100 polymers contained in the library of polyarylates, Kohn et al developed a series of new assays for the rapid characterization of large polymer sets. [8,27,[36][37][38][39][40] With only 30 mg of polymer sample, glass transition temperature, melting temperature, decomposition temperature, the position of low-temperature transitions, an estimate of the flexural modulus, surface hydrophobicity, surface chemical composition (by XPS) and surface topography by scanning electron microscopy (SEM) and/or atomic force microscopy (AFM) were obtained. Using miniaturized compression-molded thin films, an additional 15 mg of polymer was sufficient to generate disks which were inserted in 96-well tissue culture plates and used to evaluate the ability of these polymers to support cell growth.…”