Characterization of Composite Nano-Bioscaffolds Based on Collagen and Supercritical Fluids-Assisted Decellularized Fibrous Extracellular Matrix

Huang, Ching-Cheng; Chen, Ying-Ju; Liu, Hsia‐Wei

doi:10.3390/polym13244326

Cited by 10 publications

(12 citation statements)

References 27 publications

(50 reference statements)

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“…All spectra are largely overlapped, indicating that there are no alterations in the structure of ECM proteins. The band between 3000 and 3600 cm −1 includes peaks of amide A due to N–H stretching (~3320 cm −1 ) and amide B caused by C–H stretching vibrations (~3020 cm −1 ) [ 51 , 52 , 53 ]. The characteristic transmittance peaks of collagen are present in the range of 1700–1250 cm −1 : band amide I at ∼1650 cm −1 and amide II at ~1560 cm −1 .…”

Section: Resultsmentioning

confidence: 99%

“…The amide I band depends on the stretching vibration of the peptide carbonyl group (–CO), while amide II is due to stretching of C–N and bending of N–H. A set of three weaker bands, which represent amide III vibration modes, is centered at ∼1245 cm −1 [ 54 ]: it is due to N–H bending [ 53 , 54 , 55 , 56 ]. In the band range of 1250–1000 cm −1 , there are typical transmittance peaks of CH and COH from carbohydrates: polycarbohydrates are the main components of glycosaminoglycans (GAGs), as reported by Jastrzebska et al [ 57 ].…”

Section: Resultsmentioning

confidence: 99%

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A New Detergent for the Effective Decellularization of Bovine and Porcine Pericardia

et al. 2022

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Human and animal pericardia are among the most widely exploited materials suitable to repair damaged tissues in the cardiovascular surgery context. Autologous, xenogeneic (chemically treated) and homologous pericardia are largely utilized, but they do exhibit some crucial drawbacks. Any tissue treated with glutaraldehyde is known to be prone to calcification in vivo, lacks regeneration potential, has limited durability, and can result in cytotoxicity. Moreover, autologous tissues have limited availability. Decellularized biological tissues represent a promising alternative: decellularization removes cellular and nuclear components from native tissues and makes them suitable for repopulation by autologous cells upon implantation into the body. The present work aims to assess the effects of a new detergent, i.e., Tergitol, for decellularizing bovine and porcine pericardia. The decellularization procedure successfully removed cells, while preserving the histoarchitecture of the extracellular matrix. No cytotoxic effect was observed. Therefore, decellularized pericardia showed potential to be used as scaffold for cardiovascular tissue regeneration.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A New Detergent for the Effective Decellularization of Bovine and Porcine Pericardia

et al. 2022

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“…To reveal the cyto-compatibility of the tested membranes, the cell viability of the L929 fibroblastic cells was investigated. The L929 cell line is one of the most frequently used lines in material/cell interaction research, and has been previously used for cytotoxicity testing for many polymeric scaffolds [ 75 , 76 , 77 ]. The cytotoxicity and wettability of the prepared membranes are summarized in Figure 5 .…”

Section: Resultsmentioning

confidence: 99%

Plasma-Initiated Grafting of Bioactive Peptide onto Nano-CuO/Tencel Membrane

Chen

Shi

et al. 2022

Polymers

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A bioactive peptide has been successfully grafted onto nano-CuO impregnated Tencel membranes by a simple and rapid method involving a series of textile processes, and an atmospheric argon plasma treatment that requires no additional solvent or emulsifier. Surface morphology shows an apparent change from smooth, slightly roughened, and stripped with increasing plasma treatment time. The FT-IR characteristic peaks confirm the presence of the CuO nanoparticle and peptide on the extremely hydrophilic Tencel membranes that exhibit a zero-degree contact angle. Prepared nano-CuO/Tencel membranes with 90 sec plasma treatment time exhibit excellent antimicrobial activity against E. coli and S. aureus, and promote fibroblast cell viability with the assistance of a grafted bioactive peptide layer on the membrane surface.

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“…Thus, gelatin hydrogels have low shape stability, poor mechanical strength, and low elasticity [ 18 ], which significantly limits their biomedical applications at physiological temperatures above 37 °C. To enhance its stability and mechanical properties, gelatin can be covalently crosslinked through the use of chemical crosslinking agents such as formaldehyde, glutaraldehyde, paraformaldehyde, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), and genipin [ 19 , 20 , 21 ]. Additionally, the use of enzymes such as tyrosinase and transglutaminase can initiate an enzymatic crosslinking procedure, causing gelatin crosslinking, as studied by [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Highly Organized Porous Gelatin-Based Scaffold by Microfluidic 3D-Foaming Technology and Dynamic Culture for Cartilage Tissue Engineering

Liu

et al. 2022

IJMS

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A gelatin-based hydrogel scaffold with highly uniform pore size and biocompatibility was fabricated for cartilage tissue engineering using microfluidic 3D-foaming technology. Mainly, bubbles with different diameters, such as 100 μm and 160 μm, were produced by introducing an optimized nitrogen gas and gelatin solution at an optimized flow rate, and N2/gelatin bubbles were formed. Furthermore, a cross-linking agent (1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide, EDC) was employed for the cross-linking reaction of the gelatin-based hydrogel scaffold with uniform bubbles, and then the interface between the close cells were broken by degassing. The pore uniformity of the gelatin-based hydrogel scaffolds was confirmed by use of a bright field microscope, conjugate focus microscope and scanning electron microscope. The in vitro degradation rate, mechanical properties, and swelling rate of gelatin-based hydrogel scaffolds with highly uniform pore size were studied. Rabbit knee cartilage was cultured, and its extracellular matrix content was analyzed. Histological analysis and immunofluorescence staining were employed to confirm the activity of the rabbit knee chondrocytes. The chondrocytes were seeded into the resulting 3D porous gelatin-based hydrogel scaffolds. The growth conditions of the chondrocyte culture on the resulting 3D porous gelatin-based hydrogel scaffolds were evaluated by MTT analysis, live/dead cell activity analysis, and extracellular matrix content analysis. Additionally, a dynamic culture of cartilage tissue was performed, and the expression of cartilage-specific proteins within the culture time was studied by immunofluorescence staining analysis. The gelatin-based hydrogel scaffold encouraged chondrocyte proliferation, promoting the expression of collagen type II, aggrecan, and sox9 while retaining the structural stability and durability of the cartilage after dynamic compression and promoting cartilage repair.

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Characterization of Composite Nano-Bioscaffolds Based on Collagen and Supercritical Fluids-Assisted Decellularized Fibrous Extracellular Matrix

Cited by 10 publications

References 27 publications

A New Detergent for the Effective Decellularization of Bovine and Porcine Pericardia

A New Detergent for the Effective Decellularization of Bovine and Porcine Pericardia

Plasma-Initiated Grafting of Bioactive Peptide onto Nano-CuO/Tencel Membrane

Highly Organized Porous Gelatin-Based Scaffold by Microfluidic 3D-Foaming Technology and Dynamic Culture for Cartilage Tissue Engineering

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