Characterization of copper/zinc and manganese superoxide dismutase in green bamboo (Bambusa oldhamii): Cloning, expression and regulation

Liao, Ming-Huei; Kuo, Wen-Yu; Huang, Chien-Hua; Hsieh, Hsu-Liang; Jinn, Tsung-Luo

doi:10.1016/j.plaphy.2010.11.011

Cited by 16 publications

(15 citation statements)

References 21 publications

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“…MgMnSOD1 is predicted to be the main MnSOD gene involved in stress response of M. 9 giganteus. The high identity ([99 %) of amino acid MgMnSOD1 showed excellent resistance to heat, which is more stable than some other MnSOD in previous reports (Khanna-Chopra et al 2011;Lai et al 2008;Wu et al 2011). The thermal stability is probably because the presence of metal cofactor Mn 2?…”

Section: Discussionmentioning

confidence: 79%

“…MgMnSOD1 was rather conserved both in the sequence and length of protein compared with those from other monocots (Saccharum officinarum, Triticum aestivum, Bambusa oldhamii, Zea mays, Oryza sativa) and dicots (Nelumbo nucifera, Arabidopsis thaliana, Pisum sativum, Helianthus annuus) by multiple alignment analysis (Dong et al 2009;Fernández-Ocaña et al 2011;Kliebenstein et al 1998;Que et al 2012;White and Scandalios 1988;Wong-Vega et al 1991;Wu et al 1997Wu et al , 2011Zhu and Scandalios 1993) (Fig. S1).…”

Section: Sequence Analyses Of Mnsod Cdnasmentioning

confidence: 96%

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Molecular cloning and characterization of two manganese superoxide dismutases from Miscanthus × giganteus

et al. 2015

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Six MnSOD genes were isolated from five Miscanthus species. MgMnSOD1 functions in mitochondria and MgMnSOD1 seems to be the main MnSOD gene involved in stress response of M. × giganteus. Miscanthus × giganteus is a promising biomass energy crop with advantages of vigorous growth, high yield, low fertilizer and pesticide inputs. However, poor overwinter ability limits its widespread cultivation. Moreover, narrow genetic base may increase the risk of susceptibility to diseases and pests. Manganese superoxide dismutase (MnSOD), an important antioxidant enzyme involved in stress tolerance is able to protect plant cells from accumulated reactive oxygen species by converting superoxide to peroxide and oxygen. In many plants, overexpression of MnSOD has shown the ability to enhance the resistance to various stresses. This article describes the studies performed in an attempt to elucidate the molecular and enzymatic properties of MnSODs in M. × giganteus. MnSOD genes from M. × giganteus (MgMnSOD1, MgMnSOD2), M. lutarioriparia (MlMnSOD), M. sacchariflora (MsaMnSOD), M. sinensis (MsiMnSOD), and M. floridulus (MfMnSOD) were cloned and sequenced. The sequence analysis and expression patterns of MgMnSOD1 and MgMnSOD2 suggest that they were orthologous genes which were inherited from the two parents, M. sacchariflora and M. sinensis, respectively. In addition, MgMnSOD1 is predicted to be the main MnSOD gene involved in stress response of M. × giganteus. The activity of purified recombinant MgMnSOD1 was 1854.79 ± 39.98 U mg(-1) (mean ± SD). Further enzymatic assays revealed that the protein exhibited an outstanding thermal stability. MgMnSOD1 is predicted to be targeted to mitochondria and involved in removing the superoxide radical generated by respiration. The presence and sequences of other SOD isozymes transcripts were also investigated in this study.

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Section: Discussionmentioning

confidence: 79%

Section: Sequence Analyses Of Mnsod Cdnasmentioning

confidence: 96%

Molecular cloning and characterization of two manganese superoxide dismutases from Miscanthus × giganteus

et al. 2015

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“…There are several ways in which plants use or rely on metals to influence the outcome of infection. There are a number of metalloenzymes that are important for plant defence (Wu et al, 2011), meaning that the availability of these metals to the plant can be an important factor in the outcome of the plant-pathogen interaction. Secondly, the plant may attempt to overwhelm the pathogen's metal homoeostasis and tolerance mechanisms by oversupplying a potentially toxic metal (Fones et al, 2010;Yuan et al, 2010).…”

Section: Metals In Plant Defencementioning

confidence: 99%

“…Two metalloenzymes of particular importance in plant-pathogen interactions are SOD, which exists as three isoforms that can be separated according to their metallic cofactors of Fe, Mn and Cu/Zn (Kliebenstein et al, 1998;Wu et al, 2011), and catalase, which relies for its activity on four heme groups (Reid et al, 1981;Willekens et al, 1995). An exhaustive review of this topic is beyond the scope of this review, but a few examples of particular note are discussed here.…”

Section: Indirect Effects Of Metals In Defencementioning

confidence: 99%

The impact of transition metals on bacterial plant disease

2013

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Metals play essential roles in many biological processes but are toxic when present in excess. This makes their transport and homoeostatic control of particular importance to living organisms. Within the context of plant-pathogen interactions the availability and toxicity of transition metals can have a substantial impact on disease development. Metals are essential for defensive generation of reactive oxygen species and other plant defences and can be used directly to limit pathogen growth. Metal-based antimicrobials are used in agriculture to control plant disease, and there is increasing evidence that metal hyperaccumulating plants use accumulated metal to limit pathogen growth. Pathogens and hosts compete for available metals, with plants possessing mechanisms to withhold essential metals from invading microbes. Pathogens, meanwhile, use low-metal conditions as a signal to recognise and respond to the host environment. Consequently, metal-sensing systems such as fur (iron) and zur (zinc) regulate the expression of pathogenicity and virulence genes; and pathogens have developed sophisticated strategies to acquire metal during growth in plant tissues, including the production of multiple siderophores. This review explores the impact of transition metals on the processes that determine the outcome of bacterial infection in plants, with a particular emphasis on zinc, iron and copper.

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“…Mutational studies in mouse and human CuZnSOD have shown to cause premature neuron death, cytoskeletal and cell cycle changes (Jaarsma et al, 2000;Takamiya et al, 2005). Numerous stress inducible isoforms of CuZnSODs have been identified and characterized from various monocot and dicot plants (Dong et al, 2011;Hernández-Nistal et al, 2002;Kernodle and Scandalios, 2001;Qu et al, 2010;Sunkar et al, 2006;Wu et al, 2011;Zhang et al, 2008). Their defensive function in plants has been explored by transgenic approaches, primarily by overexpressing CuZnSOD and relating its expression to the degree of plant's oxidative stress tolerance in turn highlighting their significance in plant's adaptation to abiotic (Gill et al, 2010;Lee et al, 2007;Prashanth et al, 2007) and biotic insults (Faize et al, 2011;Tertivanidis et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Biochemical and molecular analyses of copper–zinc superoxide dismutase from a C4 plant Pennisetum glaucum reveals an adaptive role in response to oxidative stress

Mahanty

Kaul

Pandey

et al. 2012

Gene

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Characterization of copper/zinc and manganese superoxide dismutase in green bamboo (Bambusa oldhamii): Cloning, expression and regulation

Cited by 16 publications

References 21 publications

Molecular cloning and characterization of two manganese superoxide dismutases from Miscanthus × giganteus

Molecular cloning and characterization of two manganese superoxide dismutases from Miscanthus × giganteus

The impact of transition metals on bacterial plant disease

Biochemical and molecular analyses of copper–zinc superoxide dismutase from a C4 plant Pennisetum glaucum reveals an adaptive role in response to oxidative stress

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