Characterization of critical factors influencing gene expression of two types of fatty acid-binding proteins (L-FABP and Lb-FABP) in the liver of birds

Murai, Atsushi; Furuse, Mitsuhiro; Kitaguchi, Kohji; Kusumoto, Kohji; Nakanishi, Yuki; Kobayashi, Misato; Horio, Fumihiko

doi:10.1016/j.cbpa.2009.06.007

Cited by 21 publications

(23 citation statements)

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“…Two types of intracellular lipidbinding proteins are abundant in the avian liver, the liver bile acid-binding protein (L-BABP), formerly called the liver "basic" fatty acid-binding protein (Lb-FABP) and the liver fatty acid binding protein (L-FABP) (Murai et al, 2009). L-BABP is isolated from chicken liver and expressed only in liver (Ceciliam et al, 1994;Murai et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Identification of SNPs of the L-BABP and L-FABP and their Association with Growth and Body Composition Traits in Chicken

et al. 2013

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Fatty acid-binding proteins (FABPs) are members of lipid-binding proteins that participate in lipid uptake and intracellular transport. This study was designed to investigate the effects of the liver bile acid-binding protein (L-BABP) and the liver fatty acid binding protein (L-FABP) genes on chicken growth and body composition traits. Three SNPs (SNP1, SNP2 and SNP3) of the L-BABP and six SNPs (SNP7-SNP9, SNP11-SNP13) of the L-FABP were detected firstly by DNA sequencing. Seven SNPs (SNP6 and SNP15-SNP20) of the L-FABP were detected by DNA sequencing and were found in the chicken genomic databases at the same time. Sequenom MassARRAY was developed to genotype 966 birds from the Northeast Agricultural University F 2 (NEAUF 2 ) resource population and the Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) population. Linkage disequilibrium analysis showed that the L-BABP SNPs appeared to be strongly linked with each other and were located within a haplotype block in both populations. SNP7 and SNP8, SNP12 and SNP13, SNP19 and SNP20 of the L-FABP were in strong linkage disequilibrium, respectively. The L-BABP polymorphisms were significantly associated with body weight (BW) (P＜0.05) in the NEAUF 2 and the NEAUHLF populations and abdominal fat weight (AFW) and abdominal fat percentage (AFP) (P＜0.05) in the NEAUHLF population. The L-BABP diplotypes were significantly associated with BW (P＜0.05) in the NEAUF 2 and the NEAUHLF populations. There was no consistent association between the thirteen SNPs of the L-FABP and the growth and body composition traits in either the NEAUF 2 or the NEAUHLF populations. The research suggests that the L-BABP could be a candidate locus to affect growth traits in chickens.

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Section: Introductionmentioning

confidence: 99%

Identification of SNPs of the L-BABP and L-FABP and their Association with Growth and Body Composition Traits in Chicken

et al. 2013

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“…In avian species, L-FABP and L-BABP are two types of intracellular lipid-binding proteins in the liver. Based on the 39% homology of amino acid sequences between chicken L-FABP and L-BABP (Murai et al, 2009), the specificities of the L-FABP and L-BABP antisera were tested by western blot analysis to avoid the presence of immune cross-reactivity between them. The result showed that each antiserum could recognize its corresponding protein, with no immunological cross-reaction.…”

Section: Discussionmentioning

confidence: 99%

“…In avian species, lipogenesis takes place primarily in the liver, accounting for 95% of the de novo FA synthesis, and there is a general assumption that almost all the fat that accumulates in broiler adipose tissue is synthesized in the liver (Griffin et al, 1992). Two types of intracellular lipid-binding proteins are abundant in the avian liver; the liver fatty acid-binding protein (L-FABP) and the liver bile acid-binding protein (L-BABP), formerly called the liver "basic" fatty acid-binding protein (Lb-FABP) (Murai et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

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Differential expression of L-FABP and L-BABP between fat and lean chickens

Zhang¹,

Shi²,

Liu³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Liver fatty acid-binding protein (L-FABP) and liver bile acid-binding protein (L-BABP), in the liver intra-cytoplasm of chicken, are members of the fatty acid-binding protein subfamily. This study was designed to analyze and compare L-FABP and L-BABP expression levels between fat and lean lines in chicken liver tissue, and to determine the relationship between their expression and lipid metabolism. Realtime polymerase chain reaction (PCR) and Western blotting were used to detect the mRNA and protein expression in liver tissue between the lean and fat lines. Real-time PCR showed that L-FABP mRNA expression in fat male chickens was higher than that in lean male chickens at 2, 3, 4, 5, 6, and 10 weeks of age (P < 0.05), and L-BABP mRNA expression in fat male chickens was higher than that in lean male chickens at 1, 2, 3, 4, 8, and 10 weeks of age (P < 0.05). Western blotting showed that the L-FABP protein expression in fat male chickens was higher than that in lean male chickens at 3, 5, 6, and 7 weeks of age (P < 0.05), and L-BABP protein expression in fat male chickens was higher than that in lean male chickens at 3, 4, 5, and 6 weeks of age (P < 0.05). These results suggested that chicken L-FABP and L-BABP affect abdominal fat deposition through differences in their expression level, and the possible Expression of chicken L-FABP and L-BABP mechanism is that a high expression level of L-FABP and L-BABP leads to a high lipogenesis rate and, ultimately, to lipid deposition.

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“…In bird, the results found that feeding stimulation was a critical factor inducing FABP gene expression irrespective of light condition, however, feeding stimulation only slightly stimulated expression of the FABP gene, and was not always its primary determinant [17]. The studies in RNAi and transgenic animals have showed that the expression of the FABPs are in many cases interdependent, FABP can be considered that grave functional consequences of the loss of a particular FABP are often prevented by the compensatory overexpression of another member of FABP family [18].…”

Section: Qrt-pcr Analysis Of Oma-fabp Expressionmentioning

confidence: 93%

Characterization of Gene Expression of Fatty Acid Binding Proteins (FABP) in Largescale Shoveljaw Fish (Onychostoma macrolepis)

Yang¹,

Xu²,

Li³

et al. 2014

IJBBB

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Abstract-The Fatty acid-binding proteins (FABP) is a 14-16 kDa conserved multifunctional protein that plays key roles in binding fatty acid ligands, metabolism of fatty acids and metabolic homeostasis. In this study, the complete cDNA of FABP was isolated and characterized from Onychostoma macrolepis to study the functions of FABP in fish metabolism, we report the FABP (GenBank number:JN254629), it contains 405 bp open reading frame(ORF) encoding predicted 134 amino acids. Phylogenetic analysis was showed that Oma-FABP shared 94% sequenced identity with Cyprinus carpio and Rutilus rutilus, suggest that FABP have conserved amino acid sequence and domains in fishes. Expression diversifications of Oma-FABP are analyzed in tissues, feeding stimulation, and starvation challenge using a qRT-PCR approach. In Onychostoma macrolepis, FABP are expressed ubiquitously although transcript levels varied between organs, the highest mRNAs levels are detected in muscle. During feeding stimulation, Oma-FABP was induced and downregulated by dietary protein of 52% in liver (P<0.05), 42% in intestine (P<0.05). In starvation challenge, Oma-FABP modifies its expression, increasing its transcripts at 2 days, 6 days and 9 days after treatment(P<0.05), the expression of Oma-FABP was highly expressed from 3d to 6d. Overall, these results demonstrate the existence of FABP, and differentially regulated by changes in conditions.

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Characterization of critical factors influencing gene expression of two types of fatty acid-binding proteins (L-FABP and Lb-FABP) in the liver of birds

Cited by 21 publications

References 30 publications

Identification of SNPs of the <i>L-BABP </i>and <i>L-FABP </i>and their Association with Growth and Body Composition Traits in Chicken

Identification of SNPs of the <i>L-BABP </i>and <i>L-FABP </i>and their Association with Growth and Body Composition Traits in Chicken

Differential expression of L-FABP and L-BABP between fat and lean chickens

Characterization of Gene Expression of Fatty Acid Binding Proteins (FABP) in Largescale Shoveljaw Fish (Onychostoma macrolepis)

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