Characterization of Cucurbit Aphid-Borne Yellows Virus (CABYV) from Passion Fruit in Brazil: Evidence of a Complex of Species within CABYV Isolates

Vidal, Andreza Henrique; Lacorte, Cristiano; Sanches, Márcio Martinello; Alves-Freitas, Dione M T; Abreu, E. F. M.; Pinheiro-Lima, Bruna; Rosa, Raul Castro Carriello; Jesus, O. N. de; Campos, Magnólia A.; Felix, Gustavo Pereira; Abreu, Ana Clara R.; Santos, Yam S.; Lacerda, A. L. M.; Varsani, Arvind; Melo, Fernando Lucas; Lefeuvre, Pierre

doi:10.3390/v15020410

Cited by 4 publications

(1 citation statement)

References 78 publications

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“…The analysis showed that the CABYV isolates formed two distinct phylogenetic clusters, with one cluster containing isolates from Africa and Asia, while the other cluster comprising isolates from Europe. Additionally, several recombination events were identified within the CABYV genome, which may have contributed to the emergence and diversification of new strains of the virus [ 14 ]. High-throughput sequencing was used to analyze the genetic diversity of CABYV isolates collected from Spain and other European countries.…”

Section: Introductionmentioning

confidence: 99%

Genetic Variation between Asian and Mediterranean Populations of Cucurbit Aphid-Borne Yellows Virus

Pouraziz,

Yousefi,

Santosa

et al. 2023

Viruses

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Viral symptoms, such as yellowing, leaf deformation, mottling, vein clearing, and reduced yield, were observed in cucurbits in Iran. This study aimed to detect the main suspected causal agent, cucurbit aphid-borne yellows virus (CABYV), in Iran and analyze the genetic diversity among isolates. Two hundred samples were collected from different growing areas between 2019 and 2022. PCR amplification was performed on the P3 and P4 genes. The sequences of 18 Iranian isolates were obtained and deposited in GenBank. Recombination, phylogenetic, and population genetics studies were then carried out for the complete genome and all ORFs sequences, together with other isolates in GenBank. The nucleotide identities of the overlapped ORF3/4 sequences of Iranian isolates were 94.8 to 99.5% among themselves, and with other tested isolates ranging from 94.3 to 99.3%. Phylogenetic trees based on the complete genome and the overlapped ORF3/4 showed two major clades, namely Asian and Mediterranean, and the new isolates from Iran were positioned in both clades. The obtained results also suggest that all the genes and two clades of CABYV populations were under negative selection pressure. Furthermore, rare gene flow between these two clades (FST > 0.33) confirmed the high genetic separation among them.

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Section: Introductionmentioning

confidence: 99%

Genetic Variation between Asian and Mediterranean Populations of Cucurbit Aphid-Borne Yellows Virus

Pouraziz,

Yousefi,

Santosa

et al. 2023

Viruses

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Detection and molecular characterization of a novel mitovirus associated with Passiflora edulis Sims

Santos,

Vidal,

Abreu

et al. 2024

Arch Virol

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Global phylodynamics of two relevant aphid-transmitted viruses in cucurbit crops: cucurbit aphid-borne yellows virus and watermelon mosaic virus

Rabadán,

Gómez

2023

Phytopathol Res

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Cucurbit aphid-borne yellows virus (CABYV) and watermelon mosaic virus (WMV) are major plant pathogens that cause severe epidemics in cucurbit crops. While there has been an increasing interest in molecular epidemiological studies on both viruses at regional scales, their phylodynamic analysis by using the temporal data at global scale remains unexplored. In this study, we implemented the Nextstrain phylodynamic approach to comprehensively examine the coat protein gene and full-length genome sequences of the CABYV and WMV worldwide populations. Our analyses reconstructed a robust phylogeny of CABYV and confirmed the occurrence of isolates grouped into three clusters (Asian, Mediterranean, and Recombinant). Nextstrain analysis suggested that CABYV epidemics likely originated in Southeast Asia in fourteenth century, while the Mediterranean population emerged in Spain in seventeenth century. We also found a high divergence between Asian and Mediterranean isolates, with low genetic diversity and scarce evidence of selection, as reflected by the lack of gene flow. Moreover, the hierarchical analysis of molecular variance revealed a significant differentiation between CABYV populations grouped by geographical location and plant host. Additionally, the global phylogenetic reconstruction of the WMV population confirmed a clear differentiation among isolates, which grouped into two clusters (classical and emergent), and Nextstrain analysis suggested that WMV epidemics were most probably originated in USA during the sixteenth century. The initial WMV population diverged in the eighteenth century, with the origin of the emergent population in the nineteenth century. Our analysis confirmed that WMV population has a polyphyletic origin, defining an extensive genetic diversity. Overall, this work provides insights into the CABYV and WMV origin and evolutionary dynamics, gaining an understanding of the global spread of both viral diseases. Additionally, the integration of all spatio-temporal CABYV and WMV data, along with their genome sequence data by open access (https://github.com/PiR92) and the Nextstrain approach, provides a valuable tool for compiling and sharing current knowledge of these viral diseases in cucurbit crops.

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Characterization of Cucurbit Aphid-Borne Yellows Virus (CABYV) from Passion Fruit in Brazil: Evidence of a Complex of Species within CABYV Isolates

Cited by 4 publications

References 78 publications

Genetic Variation between Asian and Mediterranean Populations of Cucurbit Aphid-Borne Yellows Virus

Genetic Variation between Asian and Mediterranean Populations of Cucurbit Aphid-Borne Yellows Virus

Detection and molecular characterization of a novel mitovirus associated with Passiflora edulis Sims

Global phylodynamics of two relevant aphid-transmitted viruses in cucurbit crops: cucurbit aphid-borne yellows virus and watermelon mosaic virus

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