Characterization of cytomegalovirus glycoproteins in a family of complexes designated gC-II with murine monoclonal antibodies

Abstract

Several murine monoclonal antibodies (MoAbs) were made to a family of human cytomegalovirus (HCMV) disulfide linked glycoprotein complexes designated gC-II. Characterization of these MoAbs by immunological methods showed that they could be divided into two groups recognizing different glycoproteins. Western blot analysis was done with immunoaffinity purified gC-II complexes. Under non-reducing conditions MoAbs from both groups recognized gC-II complexes with molecular weights of 67-93 k and 130 k to greater than 200 k. When purified gC-II complexes were reduced and individual glycoproteins separated by SDS-PAGE prior to Western blotting, Group 1 MoAbs reacted with glycoproteins having molecular weights of 47-63k, while Group 2 MoAbs reacted with glycoproteins having molecular weights of 39-48k and 90k to greater than 200k. Thus, gC-II complexes contain glycoproteins recognized by both groups of MoAbs. By Coomassie blue staining and incorporation of [3H]Arg, Group 1 glycoproteins appeared to be minor components in the complexes relative to Group 2 glycoproteins. Surface labeling of extracellular virus with galactose oxidase and tritiated borohydride showed that gC-II complexes of all molecular weights were on the surface of the virus. However, the most heavily labeled gC-II glycoproteins had molecular weights of 47-63k. These data confirm our previous observations that the gC-II complexes of HCMV are comprised of a heterogeneous family of glycoproteins.

The Human Cytomegalovirus UL100 Gene Encodes the gC-II glycoproteins Recognized by Group 2 Monoclonal Antibodies

“…The ascites was collected and screened for antibody to the peptides. The gC-II Group 1 MAbs 8B4 and 9El0 and the gC-II Group 2 MAbs 12G9, 15G5, 15F9, 27B4, 25C8, 23B10 and 40B7 used in this study were described previously (Kari et al, 1990).…”

“…Group 1 MAbs recognize a glycoprotein which forms a broad band in SDS-PAGE covering Mrs of 47 000 to 63 000. Group 2 MAbs recognize glycoproteins with MrS of 48000 to 200000 (Kari et al, 1990). The glycoproteins recognized by the Group 1 and Group 2 MAbs can also be differentiated by their glycan composition.…”

Processing of human cytomegalovirus envelope glycoproteins in and egress of cytomegalovirus from human astrocytoma cells

“…MAbs 15F9 and 27B4 recognized gC-II disulphide-linked glycoprotein complexes and have been previously described (Karl et al, 1990a). Glycoprotein complexes containing the HCMV gB glycoprotein were immunoprecipitated with MAb 41C2 (Lussenhop et al, 1988).…”

Complex Formation by Glycoproteins M and N of Human Cytomegalovirus: Structural and Functional Aspects

“…The gM/gN complex of betaherpesviruses, more specifically that of HCMV, represents the disulfide complex previously designated gCII (18). Early studies identified this disulfidelinked complex, but definitive evidence that it was composed of gM and gN has been presented only recently (25).…”