Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains

Mendoza, Héctor; Jara, Adelina de la; Freijanes, Karen; Carmona, Lourdes; Ramos, A.; Duarte, Vanessa de Sousa; Varela, João

doi:10.2225/vol11/issue4-fulltext-2

Cited by 10 publications

(16 citation statements)

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“…Conventional methods for screening mutated strains with high carotenoid content require manual inspection of every colony, which is time-consuming and inefficient [ 22 ]. After many mutated strains have been created, efficient screening of the desired phenotypes is the critical step and a major bottleneck in mutagenesis applications.…”

Section: Introductionmentioning

confidence: 99%

Chemical Mutagenesis and Fluorescence-Based High-Throughput Screening for Enhanced Accumulation of Carotenoids in a Model Marine Diatom Phaeodactylum tricornutum

et al. 2018

Marine Drugs

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Diatoms are a major group of unicellular algae that are rich in lipids and carotenoids. However, sustained research efforts are needed to improve the strain performance for high product yields towards commercialization. In this study, we generated a number of mutants of the model diatom Phaeodactylum tricornutum, a cosmopolitan species that has also been found in Nordic region, using the chemical mutagens ethyl methanesulfonate (EMS) and N-methyl-N′-nitro-N-nitrosoguanidine (NTG). We found that both chlorophyll a and neutral lipids had a significant correlation with carotenoid content and these correlations were better during exponential growth than in the stationary growth phase. Then, we studied P. tricornutum common metabolic pathways and analyzed correlated enzymatic reactions between fucoxanthin synthesis and pigmentation or lipid metabolism through a genome-scale metabolic model. The integration of the computational results with liquid chromatography-mass spectrometry data revealed key compounds underlying the correlative metabolic pathways. Approximately 1000 strains were screened using fluorescence-based high-throughput method and five mutants selected had 33% or higher total carotenoids than the wild type, in which four strains remained stable in the long term and the top mutant exhibited an increase of 69.3% in fucoxanthin content compared to the wild type. The platform described in this study may be applied to the screening of other high performing diatom strains for industrial applications.

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Section: Introductionmentioning

confidence: 99%

Chemical Mutagenesis and Fluorescence-Based High-Throughput Screening for Enhanced Accumulation of Carotenoids in a Model Marine Diatom Phaeodactylum tricornutum

et al. 2018

Marine Drugs

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“…Kemudian perlakuan (B) dengan kandungan karotenoid 0,8332 µg/ml, diikuti perlakuan (A) dengan kandungan karotenoid 0,4368 µg/mL (Gambar 5). Perbedaan kandungan karotenoid yang dihasilkan antar perlakuan karena adanya perbedaan kepadatan sel selama kultur (Agustini, 2010).Menurut Mendoza et al (2008), pembentukan karotenoid pada mikroalga dipengaruhi oleh beberapa parameter antara lain kecepatan pembelahan sel, kepadatan sel, produksi biomassa, perbandingan jumlah klorofil dan ukuran sel. (Shabana et al, 2016); c (Kusdarwati et al, 2011) …”

Section: H a S I L D A N P E M B A H A S A Nunclassified

PENGARUH INTENSITAS CAHAYA TERHADAP PERTUMBUHAN DAN KANDUNGAN KAROTENOID <em>Dunaliella</em> sp. PADA MEDIA EKSTRAK DAUN LAMTORO <em>Leucaena leucocephala</em>

2017

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“…Dunaliella strains are currently used for commercial production of β-carotene [13]. Although many researchers have studied Dunaliella salina [7,[14][15][16][17][18][19][20][21][22][23][24][25][26][27], no fully parametric data have been published on a single strain to facilitate development of a strain-specific computational model.…”

Section: Background and Motivationmentioning

confidence: 99%

Formation of algae growth constitutive relations for improved algae modeling.

Gharagozloo¹,

Drewry²

2013

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This SAND report summarizes research conducted as a part of a two year Laboratory Directed Research and Development (LDRD) project to improve our abilities to model algal cultivation. Algae-based biofuels have generated much excitement due to their potentially large oil yield from relatively small land use and without interfering with the food or water supply. Algae mitigate atmospheric CO 2 through metabolism. Efficient production of algal biofuels could reduce dependence on foreign oil by providing a domestic renewable energy source. Important factors controlling algal productivity include temperature, nutrient concentrations, salinity, pH, and the light-to-biomass conversion rate. Computational models allow for inexpensive predictions of algae growth kinetics in these non-ideal conditions for various bioreactor sizes and geometries without the need for multiple expensive measurement setups. However, these models need to be calibrated for each algal strain. In this work, we conduct a parametric study of key marine algae strains and apply the findings to a computational model. 4 ACKNOWLEDGMENTSThe authors of this SAND report acknowledge the following people who provided additional assistance to enable the success of the project:Todd Lane -guidance with lab-scale growth measurements Pam Lane -guidance with lab-scale growth measurements Scott James -guidance with EFDC and CE-QUAL models Jerilyn Timlin -providing greenhouse and raceway data and general guidance Ben Wu -program development Neal Fornaciari -program development Blake Simmons -program development Greg Wagner -program support Ryan Davis -Dunaliella salina guidance Dave Brekke -help procuring lab space for lab-scale measurements Aaron Collins -conducting greenhouse and absorptivity measurements Howland Jones -absorptivity calculations Kathy Alam -absorptivity measurements Laura Martin -absorptivity measurements 5

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Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains

Cited by 10 publications

References 1 publication

Chemical Mutagenesis and Fluorescence-Based High-Throughput Screening for Enhanced Accumulation of Carotenoids in a Model Marine Diatom Phaeodactylum tricornutum

Chemical Mutagenesis and Fluorescence-Based High-Throughput Screening for Enhanced Accumulation of Carotenoids in a Model Marine Diatom Phaeodactylum tricornutum

PENGARUH INTENSITAS CAHAYA TERHADAP PERTUMBUHAN DAN KANDUNGAN KAROTENOID <em>Dunaliella</em> sp. PADA MEDIA EKSTRAK DAUN LAMTORO <em>Leucaena leucocephala</em>

Formation of algae growth constitutive relations for improved algae modeling.

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