Characterization of Edwardsiella tarda Isolated from Farm-Cultured Eels, Anguilla japonica, in the Republic of Korea

Joh, Seong-Joon; Kim, Minjeong; Kwon, Hyuk-Man; Ahn, Eun-Hye; Jang, Hwan; Kwon, Jun-Hun

doi:10.1292/jvms.10-0252

Cited by 44 publications

(30 citation statements)

References 10 publications

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“…Additionally, E. tarda can be classified into two biotypes (wild and biogroup 1) based on the production of H 2 S as described in previous studies (Joh et al . ). However, the biogrouping may be a limited discriminating tool among phenotypic characteristics owing to the diversity of biochemical characteristics.…”

Section: Resultsmentioning

confidence: 97%

Phenotypic traits, virulence-associated gene profile and genetic relatedness of Edwardsiella tarda isolates from Japanese eel Anguilla japonica in Korea

Kim

Kang

Park

et al. 2013

Lett Appl Microbiol

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Significance and Impact of the Study: Based on the biochemical characteristics, the phylogenetic property of the 16S rRNA gene and PFGE types of Edwardsiella tarda, we could identify the intraspecific diversity of isolates from Japanese eel, Anguilla japonica in Korea. In addition, this study describes the strong congruence of virulence-related genes and pathogenicity, suggesting that the virulence profile may be useful tool for prediction of pathogenicity. AbstractEdwardsiella tarda is the predominant bacterium in farm-cultured eel in Korea. Here, we evaluated the heterogeneity of 37 E. tarda isolates derived from Japanese eel with various origins (olive flounder, common carp and ornamental fish) between 2003 and 2010. Regardless of origins, the biochemical characteristics of E. tarda isolates were homogenous except hydrogen sulfide production, citrate utilization and mannitol fermentation. Based on the phylogenetic analysis of 16S rRNA, E. tarda isolates could be classified into two subgroups and displayed a close relation with Edwardsiella ictaluri and Edwardsiella hosinae lineages, suggesting that the subgroup I has been a predominant type in the Jeonnam and Jeonbuk provinces. I-CeuI-based pulsed-field gel electrophoresis (PFGE) typing showed that the isolates from Japanese eels belonged to 11 pulsotypes, indicating that the presence of highly genomic diversity. Additionally, two isolates, ET-060 and ET-191, showed a high frequency of virulence genes (100%) and caused 90% and 60% mortality in Japanese eel, respectively. This finding suggests a substantial congruence of virulence gene profiles and pathogenicity. Our results demonstrate that the intraspecific diversity within E. tarda strains from Japanese eel has been in prior existence.

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Section: Resultsmentioning

confidence: 97%

Phenotypic traits, virulence-associated gene profile and genetic relatedness of Edwardsiella tarda isolates from Japanese eel Anguilla japonica in Korea

Kim

Kang

Park

et al. 2013

Lett Appl Microbiol

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“…Some previous study about the disease that usually attacks the eel is bacteria. Joh et al (2010) have reported the characteristics of Yersinia ruckeri bacteria isolated from a rearing pool of Anguilla japonica in Korea. In addition to Yersinia ruckeri, other pathogenic bacteria that attacked the A.japonica in Korea was Edwardsiella tarda, Aeromonas hydrophila, Aeromonas salmonicida, Aeromonas veronii, Streptococcus iniae, Citrobacter friend, and Vibrio alginolyticus (Joh et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Characterization of pathogenic bacteria in eel Anguilla bicolor bicolor

Wahjuningrum¹,

Hidayat²,

Budiardi³

2018

Jurnal Akuakultur Indonesia

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This research aimed to characterize bacteria caused disease in eel Anguilla bicolor bicolor. The research was conducted in two steps. The first step included the isolation and identification of bacteria from the disease infected glass eel (average body length: 5.0 ± 0.5 cm, average weight: 0.5 ± 0.1 g). The observation were colony and cell morphology, physiology, and biochemical characterization of bacteria, hemolysis test, and bacteria identification performed by KIT API 20 E, KIT API 20 Strep, and KIT API 20 Listeria. The second step was Koch's postulate, tested on healthy elver with an average length of 15.00 ± 0.65 cm and weight of 3.00 ± 0.75 g. The results showed three dominant species of bacteria suspected as a causative agent in eel, namely: Aeromonas hydrophila, Streptococcus agalactiae, and Listeria grayi. Koch's postulates test proved that the Aeromonas hydrophila and Streptococcus agalactiae were virulent to Anguilla bicolor bicolor. Thus, A. hydrophila and S. agalactiae were disease-causing agent bacteria in eel.Keywords: Anguilla bicolor bicolor, bacteria, A. hydrophila, S. agalactiae. ABSTRAKPenelitian ini bertujuan untuk mengkarakterisasi bakteri penyebab penyakit pada ikan sidat Anguilla bicolor bicolor. Penelitian ini dilakukan dalam dua tahap. Tahap pertama meliputi isolasi dan identifikasi bakteri dari ikan sidat kondisi sakit pada stadia glass eel. Ukuran panjang ikan sidat rata-rata 5 ± 0,5 cm dan bobot ratarata 0,5 ± 0,08 g, pengamatan bentuk morfologi koloni dan morfologi sel, karakterisasi fisiologi, dan biokimia bakteri, serta uji hemolisis, dan identifikasi jenis bakteri dengan KIT API 20 E, KIT API 20 Strep, dan KIT API 20 Listeria. Tahap kedua yaitu uji postulat Koch pada ikan sidat kondisi sehat stadia elver yang berukuran panjang rata-rata 15 ± 0,65 cm dan bobot rata-rata 3 ± 0,75 g. Hasil penelitian diperoleh tiga jenis bakteri dominan yaitu Aeromonas hydrophila, Streptococcus agalactiae, dan Listeria grayi. Uji postulat Koch membuktikan bahwa bakteri A. hydrophila dan S. agalactiae bersifat virulen pada ikan sidat Anguilla bicolor bicolor. Dengan demikian maka bakteri A. hydrophila dan S. agalactiae sebagai bakteri penyebab penyakit pada ikan sidat.Kata kunci: Anguilla bicolor bicolor, bakteri, A. hydrophila, S. agalactiae

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“…E. tarda has been recognised as one of the major bacterial pathogens to freshwater-and marine-farmed fish species worldwide (Xu and Zhang 2014). In Korea, China and Japan olive flounder and eels are main species that are affected by E. tarda in (Kanai et al 1988;Han et al 2006;Joh et al 2010;Xu and Zhang 2014). E. tarda has also been reported as a rare human pathogen.…”

Section: Introductionmentioning

confidence: 99%

Epidemiological cut-off values for disc diffusion data generated by standard test protocols from Edwardsiella tarda and Vibrio harveyi

et al. 2016

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The susceptibility of 52 isolates of Edwardsiella tarda and 54 isolates of Vibrio harveyi to 12 antibiotics was established using internationally standardised disc diffusion protocols. Normalised resistance interpretation was employed to generate statistically valid epidemiological cut-off values for these susceptibility data. For three of these 24 species/ antibiotic data sets, there were insufficient fully sensitive strains to allow analysis and for an additional two there were sufficient fully susceptible isolates to allow only a provisional estimate to be made. The data sets for a further two species/antibiotic combinations were considered too diverse to allow the calculation of a valid cut-off value. Valid epidemiological cut-off values were, therefore, calculated for 17 species/antibiotic combinations. It is argued that these cut-off values will greatly facilitate the performance of studies designed to monitor the consequence of antibiotic use in aquaculture. They will also facilitate the prudent and rational choice of antibiotics to be applied therapeutically in commercial farming of aquatic animals.

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Characterization of Edwardsiella tarda Isolated from Farm-Cultured Eels, Anguilla japonica, in the Republic of Korea

Cited by 44 publications

References 10 publications

Phenotypic traits, virulence-associated gene profile and genetic relatedness of Edwardsiella tarda isolates from Japanese eel Anguilla japonica in Korea

Phenotypic traits, virulence-associated gene profile and genetic relatedness of Edwardsiella tarda isolates from Japanese eel Anguilla japonica in Korea

Characterization of pathogenic bacteria in eel Anguilla bicolor bicolor

Epidemiological cut-off values for disc diffusion data generated by standard test protocols from Edwardsiella tarda and Vibrio harveyi

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