“…EmpA and PrtV belong to the M4 and M6 family of peptidases, respectively, and are translated in inactive form which passes through a maturation process involving proteolytic cleavages during secretion through the T2SS ( Milton et al, 1992 ; Staroscik et al, 2005 ; Zhang et al, 2006 ; Varina et al, 2008 ; Mo et al, 2010 ; Rompikuntal et al, 2015 ). Besides its proteolytic activity, EmpA activates hemolysins promoting disruption of red blood cells in fish ( Han et al, 2011 ). PrtV contains gelatinase, protease and glycosidase activity, and a prtV mutant of V. anguillarum strain show reduced infection, growth and hemolytic activity in turbot and turbot cell lines ( Mo et al, 2010 ).…”