Characterization of enzymatically digested hyaluronic acid using NMR, Raman, IR, and UV–Vis spectroscopies

Alkrad, Jamal Alyoussef; Mrestani, Yahya; Stroehl, Dieter; Wartewig, S.; Neubert, R.

doi:10.1016/s0731-7085(02)00682-9

Cited by 121 publications

(91 citation statements)

References 14 publications

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“…Hydrolysis of Na 2 S increased the pH of the reaction mixture above 7. Under such conditions, the acetamido groups could split apart from the degradation typical for polysaccharides [65] in alkaline media. Electric resistivity studies performed for the Hyal foil in the range of 100-1000 V revealed a perfect linearity of the current-voltage (I-V) characteristic (see Fig.…”

Section: Resultsmentioning

confidence: 99%

CdS and ZnS quantum dots embedded in hyaluronic acid films

Khachatryan

Stobiński

et al. 2009

Journal of Alloys and Compounds

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Section: Resultsmentioning

confidence: 99%

CdS and ZnS quantum dots embedded in hyaluronic acid films

Khachatryan

Stobiński

et al. 2009

Journal of Alloys and Compounds

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“…HMWHA forms a viscous, pericellular meshwork around cells in a dose-dependent manner, restricting movement of reactive oxygen species [27]. Moreover, recent spectroscopy studies allowing enzymatic digestion of HA indicated the presence of a double bond in the D-glucuronic acid unit [28] which can form a complex with reactive oxygen species and reduce the toxicity of radicals. Exposing liposomal skin lipids to UV irradiation, HA and its fragments exerted antioxidative effects [29] which supports the influence of the function as a radical scavenger.…”

Section: Radical Scavengermentioning

confidence: 99%

Hyaluronic Acid in the Treatment and Prevention of Skin Diseases: Molecular Biological, Pharmaceutical and Clinical Aspects

Weindl

Schaller²,

Schäfer‐Korting³

et al. 2004

Skin Pharmacol Physiol

168

128

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The glycosaminoglycan hyaluronic acid (HA), or hyaluronan, is a major component of the extracellular matrix of skin, joints, eye and many other tissues and organs. In spite of its simple structure, HA demonstrates remarkable rheological, viscoelastic and hygroscopic properties which are relevant for dermal tissue function. Biological activities in skin, however, are also due to its interaction with various binding proteins (hyaladherins). Due to an influence on signaling pathways, HA is involved in the wound-healing process and scarless fetal healing. Increased HA concentrations have been associated with inflammatory skin diseases. In clinical trials, topical application of HA improved wound healing; in particular, acute radioepithelitis, venous leg ulcers or diabetic foot lesions responded to HA treatment. Moreover, as a topical drug delivery system for diclofenac, an HA gel has recently been approved for the treatment of actinic keratoses. Finally, chemical modifications led to new HA derivates and biomaterials, which may be introduced into therapy in the future. Therefore, ongoing research offers new horizons for the therapeutic use of this glycosaminoglycan which has been regarded as an inert structural component until recently.

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“…In our experiments, we used high specific hyaluronan lyase produced by Streptomyces hyalurolyticus (Eppig 1980;Vanderhyden 1993), and a mixture of 2HA and longer HA oligomers, in general called β-elimination products, were produced and subsequently measured. Only double bounds of SHH-derived 2HA/HA oligomers are capable of absorbing UV light (Alkrad et al 2003). However, our spectrophotometric analysis showed lower absorbance values of SHH-digested HA polymer compared with undigested 4-6HA.…”

Section: Discussionmentioning

confidence: 50%

“…In addition to HPLC improvement, optimization of our measurement wavelength was performed, and 216 nm was established as the optimal wavelength and subsequently used. A different optimal wavelength compared with the initially tested 232 nm has been confirmed by previous studies (Takagaki et al 1994;Alkrad et al 2003), which describe various wavelengths for spectrophotometric measurement of HA-digestion product, pointing out divergent laboratory conditions. Overall, the results suggest that the original points show the means of three independent experiments ± SEM GLM procedure followed by t-test was performed and different letters indicate significant differences (P < 0.05) Figure 7.…”

Section: Discussionmentioning

confidence: 52%

A simple method for assessing hyaluronic acid production by cumulus-oocyte complexes

Zámostná¹,

Nevoral²,

Kott³

et al. 2016

CZECH J ANIM SCI

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ABSTRACT:The cumulus expansion of cumulus-oocyte complex (COC) is an essential regulating process of oocyte maturation and as such it is a possible biomarker of the in vitro maturing oocytes quality. Cumulus expansion is usually assessed by non-invasive methods based on visual evaluation with many inaccuracies. On the other hand, analytical measurement of the quantity of hyaluronic acid (HA), the most abundant compound of expanded cumuli, is one of possible methods to evaluate cumulus expansion precisely. Therefore, this study aimed to verify the applicability of HA analysis for evaluating the cumulus expansion and testing oocyte maturation. The COCs were cultured in modified M199 medium for 8-48 h. The samples for the HA analysis were prepared on an 8-hour time scale, and HA retained in COCs was measured using a spectrophotometric method adapted for this purpose. We observed an increasing quantity of HA during the in vitro cultivation. A comparison with expanded COCs' classification or expansion area proved the proposed method of HA analysis suitable for the evaluation of cumulus expansion in vitro. Our findings consider the quantity of HA-expressed cumulus expansion to be a valuable marker of COC quality enabling an adequate oocyte meiotic stage estimation.

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Characterization of enzymatically digested hyaluronic acid using NMR, Raman, IR, and UV–Vis spectroscopies

Cited by 121 publications

References 14 publications

CdS and ZnS quantum dots embedded in hyaluronic acid films

CdS and ZnS quantum dots embedded in hyaluronic acid films

Hyaluronic Acid in the Treatment and Prevention of Skin Diseases: Molecular Biological, Pharmaceutical and Clinical Aspects

A simple method for assessing hyaluronic acid production by cumulus-oocyte complexes

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