Characterization of Escherichia coli Isolates from Commercial Layer Hens with Salpingitis

Abstract

Problem statement: Escherichia coli isolates are the most common bacterial agents of salpingitis in commercial layer hens. The objectives of this study were to determine the phylogenetic groups/subgroups and antibiotic resistance of E. coli isolates from salpingitis cases in commercial layer hens farms in southeast of Iran. Approach: One hundred twenty one E. coli isolates from oviducts of layer hens with salpingitis were examined to determine their O-serogroup, phylogenetic group/subgroup and antibiotic resistance pattern. Results: O-serogroup determination test showed that 47 (38.84%) isolates were typeable and belonged to eleven different O serogroups including: O1, O2, O6, O8, O15, O20, O25, O36, O78, O86 and O111 and 71 (58.67%) isolates were O-nontypeable. Three most prevalent serogroup were O78, O2 and O1 respectively. PCR assays showed that the isolates fall into four phylogenetic groups A (41.32%), D (33.88%), B2 (14.87%) and B1 (9.91%). Ninety nine (81.81%) isolates fell into six phylogenetic subgroups including: A 0 (17.35%), A 1 (23.96%), B2 2 (5.78%), B2 3 (9.09%), D 1 (13.22%) and D 2 (20.66%). All of the E. coli isolates from O78 serogroup belonged to A phylo-group whereas the O2 isolates mostly fell into D group. The maximum antibiotic resistance was against tetracycline (100%) and minimum resistance was against linco-spectin (37.19%). Twenty four isolates (19.83%) were resistant to all of the examined antibiotics. Twenty three different patterns of multiple drug resistance were observed, out of which N-Te-Sxt-Fm-Nfx-C and N-Te-Sxt-Gm-Fm-Nfx-C-Ls was the two most frequent patterns respectively. The resistant isolates were found in all of the phylogenetic groups and or sub groups. Conclusion: There are similarities between salpingitis derived E. coli strains and other avian pathogenic E. coli isolates in phylogroups, O-serogroups and antibio-resistance patterns.

Serotayping and Sensitivity Tests of Pathogenic Escherichia Coli Isolated From Salpingitis in Commercial Laying Hens.

“…It was detected in oviducts of commercial layers as one of the most predominant serovars in different studies (Trampel et al, 2007;Salehi andGhanbarpour, 2010 andSrinivasan et al, 2013). The serogroup O86 was deteced in salpingitis cases in this study and in study of Salehi andGhanbarpou (2010). Eidand Erfan (2013) found that, O114, O125, O55, O111 and O26 serogroups were the most prevalent in broiler flocks in Sharkia Governorate (Egypt), also these serogoups were found predominant in our study.…”

“…The serogroups include O111, O26, O125,O55, O114, O127, O86, O124 and O142 serogroups (Table ,2). Salehi and Ghanbarpour, (2010) isolated 47 strains of E. coli from commercial layer hens with salpingitis. The isolates were typeable and belonged to 11 serogroupes (O1, O2,O6, O8, O15, O20, O25, O36, O78, O86 and O111).…”

“…These findings suggest that salpingitis in the former bird may be caused by ascending infection of the oviduct from the cloacae and in the remaining birds may occur as part of systemic infection. Salehi and Ghanbarpour, (2010) isolated E.coli from commercial layer hens with salpingitis. The O-serogroup determination test showed that 47 (38.84%) isolates were typeable and belonged to eleven different O serogroups (O1, O2,O6, O8, O15, O20, O25, O36, O78, O86 and O111) while 71 (58.67%) isolates were O-nontypeable.…”

Characterization of E. coli and Salmonella spp. isolates associated with omphalitis in baby chicks

“…Serotyped strains (n=40) belonged to 10 serovars, O78 (n=13), O153 (n=6), O168 (n=5), O26 (n=4), O157 and O146 (n=3 each), O20 and O114 (n=2 each), O125 and O126 (n=one each). Salehi and Ghanbarpour (2010) isolated E. coli serogroups O6, O15, O25, and O78 from oviducts of layer hens with salpingitis. Meanwhile, Kika et al (2013) isolated E. coli serogroups O15 and O115 from visceral organs of birds with colibacillosis.…”

Prevalence and pathology of oviduct impaction in commercial white leghorn layer chicken in Namakkal region of India

“…Among the different serotypes identified from impacted oviduct, serotypes O , O and O were predominant, however none of the serogroup was belongs to the systemic form serogroups such as O , O and O [26,27] . The occurrence of a specific serotype and its role in disease production depends upon the health status of the birds, climatic conditions, geographical situations and managemental strategies.…”