Characterization of expressed genes in the establishment of arbuscular mycorrhiza between Amorpha fruticosa and Glomus mosseae

Aims The present study aimed at analysing the proteome pattern of the leaf blade of barley (Hordeum vulgare L.) in Serendipita indica‐colonised plants to decipher the molecular mechanism of S. indica‐mediated salt stress. This work is aligned with our previous research on barley leaf sheath to study proteomic pattern variability in leaf blade and sheath of barley plant in response to salinity and S. indica inoculation. Methods and Results The experiment was conducted using a completely randomised factorial design with four replications and two treatments: salinity (0 and 300 mmol l−1 NaCl) and fungus (noninoculated and S. indica‐inoculated). The leaf blades of the salt‐treated S. indica‐colonised and noninoculated plants were harvested after 2 weeks of salt treatment for the physiological and proteomic analyses. After exposure to 300 mmol l−1 NaCl, shoot dry matter production in noninoculated control plants decreased 84% which was about twofold higher than inoculated plants with S. indica. However, the accumulation of sodium in the shoot of S. indica‐inoculated plants was significantly lower than the control plants. Analysis of the proteome profile revealed a high number of significantly up‐regulated proteins involved in photosynthesis (26 out of 42 identified proteins). Conclusions The results demonstrated how the enhanced plant growth and salt stress resistance induced by S. indica was positively associated with the up‐regulation of several proteins involved in photosynthesis and carbohydrate metabolism. In fact, S. indica improved photosynthesis in order to reach the best possible performance of the host plant under salt stress. Significance and Impact of the Study Current research provides new insight into the mechanism applied by S. indica in reducing the negative impacts of salt stress in barley at physiological and molecular levels.

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“…Song et al . (2014) suggested the role GLP in defense during the interaction of AMF G. mosseae and A. fruticose .…”

Section: Discussionmentioning

confidence: 99%

Root endophytic fungus Serendipita indica modulates barley leaf blade proteome by increasing the abundance of photosynthetic proteins in response to salinity

et al. 2021

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“…Germination was conducted in an incubator at 30 °C for 60 h after soaking for 24 h. The growing medium was 50% peat soil, 30% vermiculite and 20% sand. It was sterilized in an autoclave at 121°C for 2 h and then air dried for 1 week before the start of the experiments 10 11 .…”

Section: Methodsmentioning

confidence: 99%

“…The expressed genes were confirmed by reverse Northern blotting. Eleven fragments were sequenced and putatively identified by homologous alignment, and these genes were found to relate to defense and signal transduction 10 . Kong xiangshi also has found 47 symbiosis-related unigenes during AMF treatment by using suppression subtractive hybridization (SSH) and subsequent Gene Ontology (GO) database, BLAST annotation and literature searches to categorize each of the identified genes.…”

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confidence: 99%

Proteomic analysis of symbiotic proteins of Glomus mosseae and Amorpha fruticosa

Song

et al. 2015

Sci Rep

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Arbuscular mycorrhiza fungi (AMF) can colonize the roots of Amorpha fruticosa, a perennial leguminous woody shrub, and form arbuscular mycorrhiza (AM). AMF have significant promoting effects on A. fruticosa growth as the intensity of fungal colonization increases. Taking AMF-A. fruticosa symbionts as the experimental material, gel-free isobaric tags for relative and absolute quantification (iTRAQ) coupled with two-dimensional liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to investigate the expression of A. fruticosa mycorrhizal proteins at the maturation stage. A total of 3,473 proteins were identified, of which 77 showed dramatic changes in their root expression levels; 33 increased, and 44 decreased. We also found nine AMF proteins that were expressed with AMF treatment. The 77 proteins were classified according to function. Plant proteins were assigned into 11 categories: metabolism-related (32%), protein folding and degradation-related (22%), energy-related (10%), protein synthesis-related (8%), stress and defense-related (24%), transcription-related (6%), membrane and transport-related (4%), cellular structure-related (2.5%), signaling transduction-related (11%) and unknown proteins (5%). The results of the study provide a foundation for further investigation of the metabolic characteristics and molecular mechanisms of AM.

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“…AMF acquire photoassimilates from plant roots, and, in return, transport essential mineral nutrients (potassium (K), phosphorus (P), nitrogen (N), etc.) from rhizosphere soil to plant roots through their arbuscules and hyphal coils ( Riley and Corradi, 2013 ; Song et al, 2014 ). Mycorrhizal hyphae attach to the root surface, infiltrate into the epidermis, and further spread into inner cortical cells to develop a highly branched arbuscular structure, where more efficient uptake of water and nutrients takes place ( Handa et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

“…The above studies investigated the positive effects of AMF inoculation on As tolerance of host plants by using phenomic approaches. The establishment of AMF symbiosis involves complex signaling events that often involve the recruitment and modification of gene expression patterns in host plants to fulfill the symbiotic requirements, and this process ultimately leads to changes in the expression of mycorrhiza-regulated genes in the host plants ( Song et al, 2014 ). RNA sequencing (RNA-Seq) is an effective method for investigating the transcriptome-wide response of plants and elucidating the underlying molecular mechanisms ( Li et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of Gossypium reveals the molecular mechanisms of Ca2+ signaling pathway on arsenic tolerance induced by arbuscular mycorrhizal fungi

Gong,

Bai,

et al. 2024

Front. Microbiol.

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IntroductionArbuscular mycorrhizal fungi (AMF) have been demonstrated their ability to enhance the arsenic (As) tolerance of host plants, and making the utilization of mycorrhizal plants a promising and practical approach for remediating As-contaminated soils. However, comprehensive transcriptome analysis to reveal the molecular mechanism of As tolerance in the symbiotic process between AMF and host plants is still limited.MethodsIn this study, transcriptomic analysis of Gossypium seedlings was conducted with four treatments: non-inoculated Gossypium under non-As stress (CK0), non-inoculated Gossypium under As stress (CK100), F. mosseae-inoculated Gossypium under non-As stress (FM0), and F. mosseae-inoculated Gossypium under As stress (FM100).ResultsOur results showed that inoculation with F. mosseae led to a reduction in net fluxes of Ca2+, while increasing Ca2+ contents in the roots and leaves of Gossypium under the same As level in soil. Notably, 199 and 3129 differentially expressed genes (DEGs) were specially regulated by F. mosseae inoculation under As stress and non-As stress, respectively. Through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation and enrichment analyses, we found that under As stress, F. mosseae inoculation up-regulated a significant number of genes related to the Ca2+ signaling pathway genes, involved in cellular process, membrane part, and signal transduction. This suggests a potential role in mitigating As tolerance in Gossypium seedlings. Furthermore, our analysis identified specific DEGs in transcription factor families, including ERF, MYB, NAC, and WRKY, that were upregulated by F. mosseae inoculation. Conversely, MYB and HB-other were down-regulated. The ERF and MYB families exhibited the highest number of up- and down-regulated DEGs, respectively, which were speculated to play an important role in alleviating the As toxicity of Gossypium.DiscussionOur findings provided valuable insights into the molecular theoretical basis of the Ca2+ signaling pathway in improving As tolerance of mycorrhizal plants in the future.

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Characterization of expressed genes in the establishment of arbuscular mycorrhiza between Amorpha fruticosa and Glomus mosseae

Cited by 9 publications

References 68 publications

Root endophytic fungus Serendipita indica modulates barley leaf blade proteome by increasing the abundance of photosynthetic proteins in response to salinity

Root endophytic fungus Serendipita indica modulates barley leaf blade proteome by increasing the abundance of photosynthetic proteins in response to salinity

Proteomic analysis of symbiotic proteins of Glomus mosseae and Amorpha fruticosa

Transcriptome analysis of Gossypium reveals the molecular mechanisms of Ca2+ signaling pathway on arsenic tolerance induced by arbuscular mycorrhizal fungi

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