Proteomic analysis of symbiotic proteins of Glomus mosseae and Amorpha fruticosa

Abstract

Arbuscular mycorrhiza fungi (AMF) can colonize the roots of Amorpha fruticosa, a perennial leguminous woody shrub, and form arbuscular mycorrhiza (AM). AMF have significant promoting effects on A. fruticosa growth as the intensity of fungal colonization increases. Taking AMF-A. fruticosa symbionts as the experimental material, gel-free isobaric tags for relative and absolute quantification (iTRAQ) coupled with two-dimensional liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to investigate the expression of A. fruticosa mycorrhizal proteins at the maturation stage. A total of 3,473 proteins were identified, of which 77 showed dramatic changes in their root expression levels; 33 increased, and 44 decreased. We also found nine AMF proteins that were expressed with AMF treatment. The 77 proteins were classified according to function. Plant proteins were assigned into 11 categories: metabolism-related (32%), protein folding and degradation-related (22%), energy-related (10%), protein synthesis-related (8%), stress and defense-related (24%), transcription-related (6%), membrane and transport-related (4%), cellular structure-related (2.5%), signaling transduction-related (11%) and unknown proteins (5%). The results of the study provide a foundation for further investigation of the metabolic characteristics and molecular mechanisms of AM.

Proteomic approach to understand the molecular physiology of symbiotic interaction between Piriformospora indica and Brassica napus

“…Song et al . 13 suggested the role of lectin and GLP in defense during the interaction of AMF G. mosseae and A. fruticose . Additionally, EP1 and lectins are involved in carbohydrate recognition and glucan binding and play an important role during pathogen attack and maintain the cell integrity 21 , 22 .…”

“…Song et al . 13 investigated the proteomes of Glomus mosseae and Amorphafruticosa interaction. Expressed proteins found extensively involved in several biological and cellular processes 14 .…”

Effect of Inoculation of Pineapple Plantlets with Arbuscular Mycorrhizal Fungi Obtained from Different Inoculum Sources Multiplied by the On-Farm Method

“…However, while low colonization was documented at 50 days in this experiment, Rodríguez-Romero et al (2011) obtained similar results seven months after inoculation with Funneliformis mosseae, but in this work, the inoculated pineapple plantlets were higher than controls in relation to SFM, SDM, and the absorption of N, P, and K. However, when producing pineapple plantlets, the average length of stay of the seedlings in the greenhouse is six months (Farahani, 2013), which is enough to gain benefits from mycorrhizal colonization, as previously demonstrated for this crop (Moreira et al, 2015(Moreira et al, , 2016. In addition, mycorrhiza might colonize a variety of agricultural crops (Cozzolino et al, 2013;Rodríguez-Romero et al, 2011), being detectable about 5-days after inoculation (Song et al, 2015).…”

Seed Metabolism and Pathogen Resistance Enhancement in Pisum sativum During Colonization of Arbuscular Mycorrhizal Fungi: An Integrative Metabolomics-Proteomics Approach

“…Even though there are increasing numbers of studies using omics technologies to investigate mycorrhizal symbiosis (Fontana et al, 2009;Pedone-Bonfim et al, 2013;Rebollo Couto et al, 2013;Laparre et al, 2014;Rivero et al, 2015;Saia et al, 2015;Bona et al, 2016;Larsen et al, 2016;Adolfsson et al, 2017;Li et al, 2017;Hill et al, 2018), the integration of several omics levels including metabolomics and proteomics with phenomics analyses is still a research gap. Also, most of this type of studies have concentrated on vegetative parts including shoot (Scheublin and van der Heijden, 2006), root (Schliemann et al, 2008;Song et al, 2015), leaf (Pedone-Bonfim et al, 2013;Schweiger et al, 2014;Desalegn et al, 2016;Adolfsson et al, 2017;Turetschek et al, 2017), and tuber (Lu et al, 2015). The current study investigates the mycorrhizal (M) impacts on above-and belowground yield and growth parameters integrating metabolomics (secondary metabolites) and proteomics analyses of seeds in two P. sativum cultivars in response to pathogen stress.…”

“…The formed symbiosis between AMF and maize (Zea mays) affected its seed proteome through modulation of enzymes related to functional categories such as stress, nucleotide metabolism, energy, storage, and development (Bona et al, 2016). Colonization of Amorpha fruticosa roots by Glomus mosseae has been modified the root proteome (Song et al, 2015). The G. mosseae by promoting cell integrity and osmotic stress depletion in wheat protected the root system against drought stress (Bernardo et al, 2017).…”