Characterization of Extended-Spectrum Beta-Lactamase–Producing<i>Salmonella enterica</i>Serotype Brunei and Heidelberg at the Hussein Dey Hospital in Algiers (Algeria)

Kermas, Rachida; Touati, Abdelaziz; Brasme, Lucien; Magrex-Debar, Elisabeth Le; Mehrane, Sadjia; Weill, François‐Xavier; Champs, Christophe de

doi:10.1089/fpd.2012.1159

Cited by 16 publications

(7 citation statements)

References 41 publications

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“…CTX-M-15 was identified in all human Salmonella strains. This finding corroborates well with several studies performed in Algeria showing that different Salmonella serotypes (Heidelberg, Kedougou, Infantis and Enteritidis) isolated from humans harbored this gene [40–43]. …”

Section: Discussionsupporting

confidence: 92%

Prevalence and clonal relationship of ESBL-producing Salmonella strains from humans and poultry in northeastern Algeria

et al. 2017

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BackgroundThe aims of this study were to investigate Salmonella contamination in broiler chicken farms and slaughterhouses, to assess the antibiotic resistance profile in avian and human Salmonella isolates, and to evaluate the relationship between avian and human Extended Spectrum β-Lactamase (ESBL)-producing isolates. Salmonella was screened in different sample matrices collected at thirty-two chicken farms and five slaughterhouses. The human isolates were recovered from clinical specimens at the University Teaching Hospital of Constantine (UTH). All suspected colonies were confirmed by MALDI-TOF (Matrix Assisted Laser Desorption Ionization Time OF light) and serotyped. Susceptibility testing against 13 antibiotics including, amoxicillin/clavulanic acid, ticarcillin, cefoxitin, cefotaxime, aztreonam, imipenem, ertapenem, gentamicin, amikacin, ciprofloxacin, colistin, trimethoprim/sulfamethoxazole and fosfomycin, was performed using the disk diffusion method on Mueller-Hinton agar. ESBL-production was screened by the double-disk synergy test and confirmed by molecular characterization using PCR (polymerase chain reaction) amplification and sequencing of ESBL encoding genes. Clonality of the avian and human strains was performed using the Multi Locus Sequencing Typing method (MLST).ResultsForty-five isolated avian Salmonella strains and 37 human collected ones were studied. Five S. enterica serotypes were found in avian isolates (mainly Kentucky) and 9 from human ones (essentially Infantis). 51.11% and 26.6% of the avian isolates were resistant to ciprofloxacin and cefotaxime, respectively, whereas human isolates were less resistant to these antibiotics (13.5% to ciprofloxacin and 16.2% to cefotaxime). Eighteen (12 avian and 6 human) strains were found to produce ESBLs, which were identified as bla CTX-M-1 (n = 12), bla CTX-M-15 (n = 5) and bla TEM group (n = 8). Interestingly, seven of the ESBL-producing strains (5 avian and 2 human) were of the same ST (ST15) and clustered together, suggesting a common origin.ConclusionThe results of the combined phenotypic and genotypic analysis found in this study suggest a close relationship between human and avian strains and support the hypothesis that poultry production may play a role in the spread of multidrug-resistant Salmonella in the human community within the study region.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-017-1050-3) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 92%

Prevalence and clonal relationship of ESBL-producing Salmonella strains from humans and poultry in northeastern Algeria

et al. 2017

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“…ESBL gene detection ( bla TEM , bla SHV , bla CTX-M and bla GES ) was screened with PCR as described previously (Poirel et al , 2000; Kermas et al , 2012) ().…”

Section: Methodsmentioning

confidence: 99%

Enterobacteriaceae isolates carrying the New Delhi metallo-β-lactamase gene in Yemen

Gharout-Sait

Alsharapy

Brasme

et al. 2014

Journal of Medical Microbiology

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Ten carbapenem-resistant Enterobacteriaceae (eight Klebsiella pneumoniae isolates and two Enterobacter cloacae) isolates from Yemen were investigated using in vitro antimicrobial susceptibility testing, phenotypic carbapenemase detection, multilocus sequence typing (MLST) and replicon typing. Carbapenemase, extended-spectrum b-lactamase (ESBL) and plasmidmediated quinolone resistance determinant genes were identified using PCR and sequencing. All of the 10 carbapenem-resistant Enterobacteriaceae were resistant to b-lactams, tobramycin, ciprofloxacin and cotrimoxazole. Imipenem, doripenem and meropenem MICs ranged from 2 to .32 mg l "1 and ertapenem MICs ranged from 6 to .32 mg l "1. All of the K. pneumoniae isolates showed ESBL activity in phenotypic tests. Genes encoding bla NDM were detected in all strains. All K. pneumoniae strains produced CTX-M-15 ESBL and SHV b-lactamases. TEM-1 b-lactamase was detected in seven isolates. Nine isolates were qnr positive including QnrB1, QnrA1 and QnrS1, and six isolates produced AAC-69-Ib-cr. MLST identified five different sequence types (STs): ST1399, ST147, ST29, ST405 and ST340. Replicon typing showed the presence of IncFII1K plasmids in four transformants. To the best of our knowledge, this is the first report of NDM-1-producing Enterobacteriaceae isolates in Yemen.

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“…The ESBL genes bla TEM , bla SHV and bla CTX-M were detected by PCR using specific primers and conditions as previously described [10]. The qnr, qepA and oqxAB genes were screened by real-time PCR as described previously [11,12].…”

Section: Detection Of Antibiotic Resistance Genesmentioning

confidence: 99%

Extended spectrum β-lactamase and plasmid mediated quinolone resistance in Escherichia coli fecal isolates from healthy companion animals in Algeria

Yousfi

Mairi

Touati

et al. 2016

Journal of Infection and Chemotherapy

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Characterization of Extended-Spectrum Beta-Lactamase–ProducingSalmonella entericaSerotype Brunei and Heidelberg at the Hussein Dey Hospital in Algiers (Algeria)

Cited by 16 publications

References 41 publications

Prevalence and clonal relationship of ESBL-producing Salmonella strains from humans and poultry in northeastern Algeria

Prevalence and clonal relationship of ESBL-producing Salmonella strains from humans and poultry in northeastern Algeria

Enterobacteriaceae isolates carrying the New Delhi metallo-β-lactamase gene in Yemen

Extended spectrum β-lactamase and plasmid mediated quinolone resistance in Escherichia coli fecal isolates from healthy companion animals in Algeria

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