2016
DOI: 10.1134/s0026261716040160
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Characterization of extracellular yeast peptide factors and their stress-protective effect on probiotic lactic acid bacteria

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Cited by 7 publications
(8 citation statements)
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“…In a next step, the relative expression ratio is determined by dividing the ratio (E ∆Ct ) by a normalization factor, which is the geometric mean of the E ∆Ct values of the most stable reference genes. The E value for each TaqMan gene expression assay (target genes and reference genes) was determined by performing qPCR reactions of a 5-log dilution range of gDNA and plotting the logarithm of the gDNA concentrations as a linear function of the Ct values [26]. From the slope of each standard curve, E was calculated as follows: The efficiencies of the TaqMan gene expression assays varied between 1.81 and 2.04 (Table S2).…”
Section: (Iv) Relative Quantification Of Gene Expression Levelsmentioning
confidence: 99%
See 1 more Smart Citation
“…In a next step, the relative expression ratio is determined by dividing the ratio (E ∆Ct ) by a normalization factor, which is the geometric mean of the E ∆Ct values of the most stable reference genes. The E value for each TaqMan gene expression assay (target genes and reference genes) was determined by performing qPCR reactions of a 5-log dilution range of gDNA and plotting the logarithm of the gDNA concentrations as a linear function of the Ct values [26]. From the slope of each standard curve, E was calculated as follows: The efficiencies of the TaqMan gene expression assays varied between 1.81 and 2.04 (Table S2).…”
Section: (Iv) Relative Quantification Of Gene Expression Levelsmentioning
confidence: 99%
“…A beneficial aspect of LAB-yeast association is the improvement of EPS production (kefiran) or bacteriocins (nisin), probably due to lactate consumption by the yeast in pH-controlled culture [21][22][23]. Co-culture with S. cerevisiae supplied alanine to L. delbrueckii subspecies bulgaricus, enhanced the viability of L. rhamnosus HN001 under acidic conditions, and enhanced the tolerance of L. paracasei H9 to simulated gastrointestinal juices as well as adhesion to intestinal mucosa cells [24][25][26].…”
Section: Introductionmentioning
confidence: 99%
“…Enterococcus faecalis as a lactic acid bacterium was separated from the commensal microbiota of human. The lactic acid bacteria are recently receiving great attentions associated with functionality [9,10]. For example, a lactic acid bacterium has recently been evaluated as a potential hospital opportunistic anti-fungal agent [11].…”
Section: Introductionmentioning
confidence: 99%
“…Comunidades biológicas estáveis são formadas quando os microrganismos em conjunto suprem as janelas metabólicas da rede, formando interações complexas de competição, mutualismo e simbiose [13]. Estas redes de interação possibilitam que os microrganismos subjuguem adversidades variegadas como estresse oxidativo ou ação de sais biliares [14], bem como podem possibilitar um aprimoramento nas capacidades probióticas de linhagens conhecidas [15,16]. Dessarte, leveduras oriundas de kombucha encerram um alto potencial biotecnológico no desenvolvimento de probióticos mais eficazes e seguros, possibilitando sua utilização como um adjuvante no desenvolvimento de novos alimentos e formulações probióticas, tal como em alimentos que apresentam uma grande variedade de microrganismos, tanto quanto os alimentos fermentados tradicionais.…”
Section: Lista De Ilustraçõesunclassified
“…Estudos sobre a interação entre bactérias e leveduras demonstram que estes microrganismos podem beneficiar-se em relações simbióticas ou mutualísticas, uma vez que estes apresentam perfis metabólicos consideravelmente distintos, sendo procariotos e eucariotos, respectivamente [14,65]. Estas interações são comuns e encontradas em diversos alimentos além da kombucha, como kefir, chucrute, chocolate (fermentação do cacau) e vinho, sendo essenciais para a manutenção do ecossistema microbiano e podem ser exploradas para beneficiar linhagens probióticas de interesse [32,66].…”
Section: Interações Entre Microrganismosunclassified