Characterization of extremely halophilic archaeal isolates from Indian salt pans and their screening for production of hydrolytic enzymes

Das, Deepthi; Kalra, Isha; Mani, Kabilan; Salgaonkar, Bhakti B.; Bragança, Judith M.

doi:10.1007/s42398-019-00077-x

Cited by 10 publications

(3 citation statements)

References 58 publications

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“…The carotenoid extracts of strains Natronococcus sp. TC6 and Halorubrum tebenquichense SU10 showed the typical bacterioruberin “three-finger” spectra [ 30 ] found in other halophilic archaea from Halobacteriaceae and Haloferacaceae families [ 31 , 32 , 33 ]. Different bacterioruberin (BAR) isomers were found in the samples of carotenoid pigments; the presence of Astaxanthin and β-carotene was confirmed.…”

Section: Discussionmentioning

confidence: 99%

Identification, Antioxidant Capacity, and Matrix Metallopeptidase 9 (MMP-9) In Silico Inhibition of Haloarchaeal Carotenoids from Natronococcus sp. and Halorubrum tebenquichense

Delgado-Garcia,

Gómez-Secundino,

Rodríguez

et al. 2023

Microorganisms

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Natural pigments from haloarchaea are of great interest; bacterioruberin is the major pigment, it shows higher antioxidant power when compared with β-carotene. However, characterization of bacterioruberin and its isomers along with its antioxidant and the matrix metallopeptidase 9 (MMP-9) inhibition activities in extracts from Natronoccoccus sp. TC6 and Halorubrum tebenquichense SU10 was not previously described, being the aim of this work. The carotenoids profile was performed by UV-Vis spectrophotometry, thin-layer chromatography, nuclear magnetic resonance spectroscopy, and high-resolution mass spectrometry (UPLC-ESI-MS/MS). Antioxidant capacity was determined for DPPH, ABTS, and FRAP. In addition, MMP-9 inhibition was studied using docking simulations. The carotenoid profile of studied strains was composed of bacterioruberin, some derivatives like mono, bis, and tris anhydrobacterioruberin, and also some bacterioruberin cis isomers. The carotenoid pools showed antioxidant capacity for DPPH > ABTS > FRAP; Natronococcus sp. TC6 carotenoid pool was better for ABTS and DPPH, while Halorubrum tebenquichense SU10 carotenoid pool was better for FRAP. Additionally, docking and molecular dynamics suggest that bacterioruberin inhibits MMP-9 through hydrophobic interactions near the catalytic site. Bacterioruberin shows the higher binding energy of −8.3 (kcal/mol). The carotenoids profile of both strains was elucidated, their antioxidant activity and singular participation of each carotenoid on MMP-9 in silico inhibition were evaluated.

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Section: Discussionmentioning

confidence: 99%

Identification, Antioxidant Capacity, and Matrix Metallopeptidase 9 (MMP-9) In Silico Inhibition of Haloarchaeal Carotenoids from Natronococcus sp. and Halorubrum tebenquichense

Delgado-Garcia,

Gómez-Secundino,

Rodríguez

et al. 2023

Microorganisms

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“…Halophiles form a group of extremophiles relatively few explored compared to thermophiles, alkalophiles and 47 acidophiles, although they have been used traditionally for the production of salt and even for the preservation of foodstuffs, few products derived from halophiles (for example, beta-carotene from Dunaliella and ectoine from moderately halophilic bacteria) have been able to enter the world market (Oren, 2010). However, no haloarchean enzyme has reached the level of production and commercial distribution to date (Das et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Diversity of Culturable Halophilic Archaea and Bacteria from Chott Tinsilt and El Malah Salt-Lake in Algeria

Akmoussi-Toumi¹,

Khemili²,

Kebbouche-Gana³

et al. 2019

Current Research in Bioinformatics

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At the limits of life, hyper-saline aquatic ecosystems; Chott and Sebkha are a model of choice of extreme environments, housing a halophilic microflora that had to adapt to these conditions. In Algeria, these ecosystems are poorly studied. However, our study was carried out on the waters of Chott Tinsilt and Sebkha El Malah. The study of this microflora revealed the presence of a significant morphological, physiological and metabolic diversity. The molecular study allowed us to access to a phylogenetic affiliation including an Archean Species (ATS1) and 7 bacterial species (A1, A2, A3, A4, B1, B4, B5). The results showed that these isolates were related to the genera Haloferax (for the strain ATS1) and Halomonas (strains A1, A2 and A4), Staphylococcus (strain A3), Salinivibrio (strain B1), Planococcus (strain B4) and Halobacillus (strain B5). Most isolates produced hydrolases at high salt concentrations. The Production yields obtained are very promising for applications in the biotechnology and industrial microbiology.

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“…They constitute predominant microbial communities in salty ecosystems, which are widespread worldwide. For example, Haloarcula, Haloferax, and Halogeometricum sp., can be isolated from saline sediments across the world, such as from Spanish coastal and inland salted ponds (Martínez et al, 2022), Algerian salt lakes, Indian salt pans, Verkhnekamsk deposit etc., and exhibit several biological activities, including the production of carotenoid pigments (Das et al, 2019;Sahli et al, 2022). Besides, many more ecosystems characterized by their high salt concentrations are far from known from a microbiological point of view (i.e., saline mines in Senegal).…”

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confidence: 99%

Bioactive molecules from haloarchaea: Scope and prospects for industrial and therapeutic applications

et al. 2023

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Marine environments and salty inland ecosystems encompass various environmental conditions, such as extremes of temperature, salinity, pH, pressure, altitude, dry conditions, and nutrient scarcity. The extremely halophilic archaea (also called haloarchaea) are a group of microorganisms requiring high salt concentrations (2–6 M NaCl) for optimal growth. Haloarchaea have different metabolic adaptations to withstand these extreme conditions. Among the adaptations, several vesicles, granules, primary and secondary metabolites are produced that are highly significant in biotechnology, such as carotenoids, halocins, enzymes, and granules of polyhydroxyalkanoates (PHAs). Among halophilic enzymes, reductases play a significant role in the textile industry and the degradation of hydrocarbon compounds. Enzymes like dehydrogenases, glycosyl hydrolases, lipases, esterases, and proteases can also be used in several industrial procedures. More recently, several studies stated that carotenoids, gas vacuoles, and liposomes produced by haloarchaea have specific applications in medicine and pharmacy. Additionally, the production of biodegradable and biocompatible polymers by haloarchaea to store carbon makes them potent candidates to be used as cell factories in the industrial production of bioplastics. Furthermore, some haloarchaeal species can synthesize nanoparticles during heavy metal detoxification, thus shedding light on a new approach to producing nanoparticles on a large scale. Recent studies also highlight that exopolysaccharides from haloarchaea can bind the SARS-CoV-2 spike protein. This review explores the potential of haloarchaea in the industry and biotechnology as cellular factories to upscale the production of diverse bioactive compounds.

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Characterization of extremely halophilic archaeal isolates from Indian salt pans and their screening for production of hydrolytic enzymes

Cited by 10 publications

References 58 publications

Identification, Antioxidant Capacity, and Matrix Metallopeptidase 9 (MMP-9) In Silico Inhibition of Haloarchaeal Carotenoids from Natronococcus sp. and Halorubrum tebenquichense

Identification, Antioxidant Capacity, and Matrix Metallopeptidase 9 (MMP-9) In Silico Inhibition of Haloarchaeal Carotenoids from Natronococcus sp. and Halorubrum tebenquichense

Diversity of Culturable Halophilic Archaea and Bacteria from Chott Tinsilt and El Malah Salt-Lake in Algeria

Bioactive molecules from haloarchaea: Scope and prospects for industrial and therapeutic applications

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