Characterization of free-living and attached bacteria in sediments colonized by Hediste diversicolor

Lucas, Françoise; Bertru, G.; Höfle, Manfred G.

doi:10.3354/ame032165

Cited by 39 publications

(25 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although it has been known that burrowing organisms affect sediment biogeochemistry, the interactions are often complex and detailed investigations on the effect of geochemical microzonations on microbial diversity are scarce (Kostka et al, 2002;Lucas et al, 2003;Matsui et al, 2004;Papaspyrou et al, 2006). This is not surprising because studies on microbial diversity in marine sediments have also only just begun (Mu mann et al, 2005;Wilms et al, 2006;Wu et al, 2008).…”

Section: Discussionmentioning

confidence: 98%

Biodiversity of benthic microbial communities in bioturbated coastal sediments is controlled by geochemical microniches

2009

View full text Add to dashboard Cite

We used a combination of field and laboratory approaches to address how the bioturbation activity of two crustaceans, the ghost shrimp Neotrypaea californiensis and the fiddler crab Uca crenulata, affects the microbial diversity in the seabed of a coastal lagoon (Catalina Harbor, Santa Catalina Island, CA, USA). Detailed geochemical analyses, including oxygen microsensor measurements, were performed to characterize environmental parameters. We used a whole-assemblage fingerprinting approach (ARISA: amplified ribosomal intergenic spacer analysis) to compare bacterial diversity along geochemical gradients and in relation to subsurface microniches. The two crustaceans have different burrowing behaviors. The ghost shrimp maintains complex, deepreaching burrows and permanently lives subterranean, supplying its burrow with oxygen-rich water. In contrast, the fiddler crab constructs simpler, J-shaped burrows, which it does not inhabit permanently and does not actively ventilate. Our goal was to address how varying environmental parameters affect benthic microbial communities. An important question in benthic microbial ecology has been whether burrows support similar or unique communities compared with the sediment surface. Our results showed that sediment surface microbial communities are distinct from subsurface assemblages and that different burrow types support diverse bacterial taxa. Statistical comparisons by canonical correspondence analysis indicated that the availability of oxidants (oxygen, nitrate, ferric iron) play a key role in determining the presence and abundance of different taxa. When geochemical parameters were alike, microbial communities associated with burrows showed significant similarity to sediment surface communities. Our study provides implications on the community structure of microbial communities in marine sediments and the factors controlling their distribution.

show abstract

Section: Discussionmentioning

confidence: 98%

Biodiversity of benthic microbial communities in bioturbated coastal sediments is controlled by geochemical microniches

2009

View full text Add to dashboard Cite

show abstract

“…Bacterial communities from the burrows of single species of nereidid worm, thalassinidean shrimp and fiddler crab (Lucas et al, 2003;Papaspyrou et al, 2005;Bertics and Ziebis, 2009, respectively) were found to be more similar to those in the surrounding subsurface sediment than to surface communities. In contrast, in this study the bacterial communities inhabiting the burrow wall more closely resembled those in surface rather than subsurface sediment.…”

Section: Figurementioning

confidence: 96%

Bioturbating shrimp alter the structure and diversity of bacterial communities in coastal marine sediments

et al. 2010

View full text Add to dashboard Cite

Bioturbation is a key process in coastal sediments, influencing microbially driven cycling of nutrients as well as the physical characteristics of the sediment. However, little is known about the distribution, diversity and function of the microbial communities that inhabit the burrows of infaunal macroorganisms. In this study, terminal-restriction fragment length polymorphism analysis was used to investigate variation in the structure of bacterial communities in sediment bioturbated by the burrowing shrimp Upogebia deltaura or Callianassa subterranea. Analyses of 229 sediment samples revealed significant differences between bacterial communities inhabiting shrimp burrows and those inhabiting ambient surface and subsurface sediments. Bacterial communities in burrows from both shrimp species were more similar to those in surface-ambient than subsurface-ambient sediment (R ¼ 0.258, Po0.001). The presence of shrimp was also associated with changes in bacterial community structure in surrounding surface sediment, when compared with sediments uninhabited by shrimp. Bacterial community structure varied with burrow depth, and also between individual burrows, suggesting that the shrimp's burrow construction, irrigation and maintenance behaviour affect the distribution of bacteria within shrimp burrows. Subsequent sequence analysis of bacterial 16S rRNA genes from surface sediments revealed differences in the relative abundance of bacterial taxa between shrimp-inhabited and uninhabited sediments; shrimp-inhabited sediment contained a higher proportion of proteobacterial sequences, including in particular a twofold increase in Gammaproteobacteria. Chao1 and ACE diversity estimates showed that taxon richness within surface bacterial communities in shrimp-inhabited sediment was at least threefold higher than that in uninhabited sediment. This study shows that bioturbation can result in significant structural and compositional changes in sediment bacterial communities, increasing bacterial diversity in surface sediments and resulting in distinct bacterial communities even at depth within the burrow. In an area of high macrofaunal abundance, this could lead to alterations in the microbial transformations of important nutrients at the sediment-water interface.

show abstract

“…data). Similarly, Lucas et al (2003) reported that the bacterial composition along the burrow walls of Nereis diversicolor is stable over time and markedly different from the community at the sediment surface. Even though microorganisms along burrow walls have to adapt to the rapidly oscillating redox environment due to the usually intermittent burrow ventilation (Kristensen 1988, Ziebis et al 1996, Furukawa 2001, our results confirm the idea that infaunal burrows, and ambient sediment, are stable environments over longterm periods compared to surface sediment, which is 188 Papaspyrou et al: Properties of Pestarella tyrrhena burrows continually disturbed by macrofaunal feeding, and current or wave mediated action (Kristensen 1988).…”

Section: Bacterial Abundance and Community Characteristicsmentioning

confidence: 99%

Sediment properties and bacterial community in burrows of the ghost shrimp Pestarella tyrrhena (Decapoda: Thalassinidea)

Papaspyrou¹,

Gregersen²,

Cox³

et al. 2005

Aquat. Microb. Ecol.

View full text Add to dashboard Cite

Chemical properties of burrow wall sediment from burrows of the thalassinidean shrimp Pestarella (=Callianassa) tyrrhena located at Vravrona Bay (Aegean Sea, Greece) were studied and found to be very different from the sediment surface and ambient anoxic sediment. P. tyrrhena burrow walls had significantly higher amounts of silt and clay, while total organic carbon (TOC) was up to 6 times higher than in surrounding sediment. Chlorophyll a (chl a) accounted for a small fraction of TOC and showed similar values in burrow walls and surface sediment, whereas the low chl a: chl a + phaeopigment ratio indicated the presence of more fresh material in the latter. Biopolymers (carbohydrates, proteins and lipids) were 4 to 11 times higher in burrow walls than in the surrounding sediment, accounting for 47% of TOC. The low protein:carbohydrate ratio indicated that the high TOC in the burrow walls was caused by the presence of aged detritus of low nutritional quality, such as seagrass detritus. The distinct conditions along the burrow wall also affected the bacterial community and resulted in a 10-fold increase of bacterial abundance. Molecular fingerprints of the bacterial communities showed that the bacterial composition of the burrow wall was more similar to the ambient anoxic sediment and showed less seasonal change than the sediment surface. These results suggest that burrow walls have distinct properties and should not be considered merely as a simple extension of the sediment surface. KEY WORDS: Bioturbation · Organic matter · Seagrass detritus · Bacteria · PCR-DGGE · Pestarella tyrrhena Resale or republication not permitted without written consent of the publisherAquat Microb Ecol 38: [181][182][183][184][185][186][187][188][189][190] 2005 ditions in the burrows (Kristensen 1988, Ziebis et al. 1996, Furukawa 2001. The availability of labile organic matter combined with steep chemical gradients and narrow redox zonation has a significant impact on the chemical and biological composition of the burrow environment. Bacterial abundances have been shown to be higher along burrow walls compared to either surface or ambient sediment (Aller & Aller 1986, Branch & Pringle 1987, Dworschak 2001. In addition, burrow walls show increased heterotrophic activity by both aerobic and anaerobic bacteria, resulting in increased rates of organic matter decomposition (Aller & Aller 1986, Reichardt 1988, Gribsholt et al. 2003.Thalassinidean ghost shrimps have been recognised in recent years as one of the most effective bioturbating groups of macrofaunal organisms, with significant impacts on the benthic environment (Griffis & Suchanek 1991, Reise 2002. Pestarella tyrrhena is an important bioturbator, commonly found in muddy and fine sandy intertidal and shallow subtidal coastal sediments, where it often creates dense monospecific populations. It is a selective deposit feeder that constructs deep and complex burrows, constantly digging new branches or filling up existing ones (Dworschak 1987). P. tyrrhena increases the organi...

show abstract

Characterization of free-living and attached bacteria in sediments colonized by Hediste diversicolor

Cited by 39 publications

References 45 publications

Biodiversity of benthic microbial communities in bioturbated coastal sediments is controlled by geochemical microniches

Biodiversity of benthic microbial communities in bioturbated coastal sediments is controlled by geochemical microniches

Bioturbating shrimp alter the structure and diversity of bacterial communities in coastal marine sediments

Sediment properties and bacterial community in burrows of the ghost shrimp Pestarella tyrrhena (Decapoda: Thalassinidea)

Contact Info

Product

Resources

About