Characterization of fructose-bisphosphate aldolase regulated by gibberellin in roots of rice seedling

Konishi, Hirosato; Yamane, Hisakazu; Maeshima, Masayoshi; Komatsu, Setsuko

doi:10.1007/s11103-004-5920-2

Cited by 112 publications

(77 citation statements)

References 33 publications

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“…Therefore, an increase and/or activation of aldolase appears to be implicated in growth mainly through the promotion of ATP synthesis in the glycolytic pathway. The involvement of FBP aldolase in cell growth was identified in rice roots treated with gibberellin [26]. In this study, FBP aldolase showed down-regulation under either chilling conditions or upon BR treatments (Fig.…”

Section: Protein Identifications and Putative Functions In The Br-medmentioning

confidence: 49%

A proteomics study of the mung bean epicotyl regulated by brassinosteroids under conditions of chilling stress

Huang

Chu

Chen

et al. 2006

Cellular and Molecular Biology Letters

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Abstract:Mung bean CYP90A2 is a putative brassinosteroid (BR) synthetic gene that shares 77% identity with the Arabidopsis CPD gene. It was strongly suppressed by chilling stress. This implies that exogenous treatment with BR could allow the plant to recover from the inhibited growth caused by chilling. In this study, we used proteomics to investigate whether the mung bean epicotyl can be regulated by brassinosteroids under conditions of chilling stress. Mung bean epicotyls whose growth was initially suppressed by chilling partly recovered their ability to elongate after treatment with 24-epibrassinolde; 17 proteins down-regulated by this chilling were re-up-regulated. These upregulated proteins are involved in methionine assimilation, ATP synthesis, cell wall construction and the stress response. This is consistent with the re-upregulation of methionine synthase and S-adenosyl-L-methionine synthetase, since chilling-inhibited mung bean epicotyl elongation could be partially recovered by exogenous treatment with DL-methionine. This is the first proteome established for the mung bean species. The regulatory relationship between brassinosteroids and chilling conditions was investigated, and possible mechanisms are discussed herein.

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Section: Protein Identifications and Putative Functions In The Br-medmentioning

confidence: 49%

A proteomics study of the mung bean epicotyl regulated by brassinosteroids under conditions of chilling stress

Huang

Chu

Chen

et al. 2006

Cellular and Molecular Biology Letters

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“…In yeast, the physiological significance of this association has not been determined, and studies have yet to decipher under what conditions the interaction takes place. In plants, an association between aldolase and V-ATPase subunits was noted in rice roots following the identification of aldolase in the tonoplast fraction (Tanaka et al, 2004); VHA-A, VHA-B, and VHA-a coimmunoprecipitated with aldolase, but regulation of activity was not demonstrated (Konishi et al, 2004(Konishi et al, , 2005, and physiological relevance was also lacking for this association.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Proteomics of the Tonoplast Reveals a Role for Glycolytic Enzymes in Salt Tolerance

et al. 2009

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To examine the role of the tonoplast in plant salt tolerance and identify proteins involved in the regulation of transporters for vacuolar Na + sequestration, we exploited a targeted quantitative proteomics approach. Two-dimensional differential in-gel electrophoresis analysis of free flow zonal electrophoresis separated tonoplast fractions from control, and salt-treated Mesembryanthemum crystallinum plants revealed the membrane association of glycolytic enzymes aldolase and enolase, along with subunits of the vacuolar H + -ATPase V-ATPase. Protein blot analysis confirmed coordinated salt regulation of these proteins, and chaotrope treatment indicated a strong tonoplast association. Reciprocal coimmunoprecipitation studies revealed that the glycolytic enzymes interacted with the V-ATPase subunit B VHA-B, and aldolase was shown to stimulate V-ATPase activity in vitro by increasing the affinity for ATP. To investigate a physiological role for this association, the Arabidopsis thaliana cytoplasmic enolase mutant, los2, was characterized. These plants were salt sensitive, and there was a specific reduction in enolase abundance in the tonoplast from salt-treated plants. Moreover, tonoplast isolated from mutant plants showed an impaired ability for aldolase stimulation of V-ATPase hydrolytic activity. The association of glycolytic proteins with the tonoplast may not only channel ATP to the V-ATPase, but also directly upregulate H + -pump activity.

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“…Fructose-bisphos- phate aldorase has been reported to be GA-inducible in rice roots, and the root growth of transgenic rice expressing antisense-RNA of the aldorase was suppressed. 18) However, none of these has been reported to have specific interaction with GAs.…”

Section: Resultsmentioning

confidence: 99%

Similarities and Differences between the Characteristics of Gibberellin-Binding Protein and Gibberellin 2-Oxidases in Adzuki Bean (Vigna angularis) Seedlings

Park

Nakajima

Hasegawa

et al. 2005

Bioscience, Biotechnology, and Biochemistry

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Gibberellin-binding proteins (GBPs) were purified ca. 230,000 fold. The characteristics of adzuki GBP were examined and compared with those of a recombinant gibberellin 2-oxidase (rVaGA2oxA1) that was fused with glutathione S-transferase (GST). VaGA2oxA1 was most abundantly expressed in etiolated adzuki bean seedlings, and VaGA2oxA1 and GBPs from adzuki bean seedlings showed gibberellin-binding activity when incubated with 2-oxoglutarate and Co 2þ . Both rVaGA2oxA1 and partially purified GBPs from adzuki bean seedlings showed very similar selectivity to gibberellins in binding assays, where biologically active gibberellins such as GA 4 , GA 3 , GA 7 , and GA 1 showed higher binding affinity than biologically inactive gibberellins such as GA 8 , GA 34 , and 3-epi-GA 4 . The polyclonal antibody raised against rVaGA2oxA1 cross-reacted with all rVaGA2oxs (rVaGA2oxA1, rVaGA2oxA2, rVaGA2oxB1, rVaGA2oxB2, and rVaGA2oxB3) whose cDNAs were cloned from adzuki bean seedlings. Treated with the antibody, the recombinants that originally showed gibberellin-binding activity lost both binding activity and enzymatic activity. In contrast to the recombinants, the gibberellin-binding activity of GBPs from adzuki bean seedlings was hardly affected by the antibody treatment. The GBPs showed very weak gibberellin 2-oxidase-like activity, and it was not affected by the antibody treatment either. These observations suggest that a major component that showed GA-binding activity was apparently different from any gibberellin 2-oxidase cloned from the seedlings.

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Characterization of fructose-bisphosphate aldolase regulated by gibberellin in roots of rice seedling

Cited by 112 publications

References 33 publications

A proteomics study of the mung bean epicotyl regulated by brassinosteroids under conditions of chilling stress

A proteomics study of the mung bean epicotyl regulated by brassinosteroids under conditions of chilling stress

Quantitative Proteomics of the Tonoplast Reveals a Role for Glycolytic Enzymes in Salt Tolerance

Similarities and Differences between the Characteristics of Gibberellin-Binding Protein and Gibberellin 2-Oxidases in Adzuki Bean (Vigna angularis) Seedlings

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