Characterization of functional properties of <i>Enterococcus faecium</i> strains isolated from human gut

İspirli, Hümeyra; Demirbaş, Fatmanur; Dertli, Enes

doi:10.1139/cjm-2015-0446

Cited by 38 publications

(24 citation statements)

References 42 publications

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“…Antibiotic resistance of LAB especially from dairy products with potential health‐promoting functions such as kefir is a major concern (İspirli et al ), and in this study, we tested the antibiotic resistance of all isolates against chloramphenicol, erythromycin, kanamycin, tetracycline, oxytetracycline, ampicillin, penicillin and streptomycin. In general, antibiotic resistance of kefir isolates was at low levels and an important proportion of the isolates were sensitive to the tested antibiotics.…”

Section: Resultsmentioning

confidence: 99%

Diversity and functional characteristics of lactic acid bacteria from traditional kefir grains

Purutoğlu

İspirli

Yüzer

et al. 2019

Int J of Dairy Tech

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Traditional kefir grains were collected from distinct parts of Turkey, and their microbial profile was determined. A wide bacterial biota was observed formed by distinct lactic acid bacteria (LAB) in which Lactococcus lactis strains appeared to be dominant. Yeast species were also identified in kefir grains. Significant levels of antifungal and antibacterial activities were monitored in kefir isolates. All tested LAB produced an exopolysaccharide (EPS) containing glucose and galactose, and some strains formed a fructan-type EPS. Importantly, low levels of antibiotic resistance were observed among the kefir isolates. (2016) Isolation of exopolysaccharide-producing bacteria and yeasts from Tibetan kefir and characterisation of the exopolysaccharides. International Journal of Dairy Technology 69 410-417. Demirbas ß F, _ Ispirli H, Kurnaz A A, Yilmaz M T and Dertli E (2017) Antimicrobial and functional properties of lactic acid bacteria isolated from sourdoughs. LWT-Food Science and Technology 79 361-366. Dertli E and C ß on A H (2017) Microbial diversity of traditional kefir grains and their role on kefir aroma. LWT-Food Science and Technology 85 151-157. Dertli E, Mercan E, Arıcı M, Yılmaz M T and Sa gdıc ß O (2016) Characterisation of lactic acid bacteria from Turkish sourdough and determination of their exopolysaccharide (EPS) production characteristics. LWT-Food Science and Technology 71 116-124. Dertli E, Colquhoun I J, Côt e G L, Le Gall G and Narbad A (2018) Structural analysis of the a-D-glucan produced by the sourdough isolate Lactobacillus brevis E25. Food chemistry 242 45-52. Franzetti L, Galli A, Pagani M and Noni I D (1998) Microbiological and chemical investigations on sugar kefir drink. Annali di Microbiologia ed Enzimologia. 48 67-80. Gao J, Gu F, Abdella N H, Ruan H and He G (2012) Investigation on culturable microflora in Tibetan kefir grains from different areas of China. Journal of food science 77 M425-M433. Garofalo C, Osimani A, Milanovi c V, Aquilanti L, De Filippis F, Stellato G and Ercolini D (2015) Bacteria and yeast microbiota in milk kefir grains from different Italian regions. Food Microbiology 49 123-133. Gul O, Atalar I, Mortas M and Dervisoglu M (2018) Rheological, textural, colour and sensorial properties of kefir produced with buffalo milk using kefir grains and starter culture: A comparison with cows' milk kefir. International Journal of Dairy Technology 71 73-80.

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Section: Resultsmentioning

confidence: 99%

Diversity and functional characteristics of lactic acid bacteria from traditional kefir grains

Purutoğlu

İspirli

Yüzer

et al. 2019

Int J of Dairy Tech

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“…Additionally, for research to successfully challenge the sterile womb paradigm, a demonstration of microbial viability is essential, as sites can be sterile even while containing bacterial DNA. Very few groups have demonstrated viable microorganisms in the fetal environment despite these studies employing culture methods that readily grow bacteria from other parts of the body [77–83]. In the case of Satokari and colleagues [84], the authors could not culture bacteria detected by molecular methods ( Bifidobacterium and Lactobacillus ) even though these are readily cultivable organisms.…”

Section: Pondering the Two Hypothesesmentioning

confidence: 99%

A critical assessment of the “sterile womb” and “in utero colonization” hypotheses: implications for research on the pioneer infant microbiome

et al. 2017

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After more than a century of active research, the notion that the human fetal environment is sterile and that the neonate’s microbiome is acquired during and after birth was an accepted dogma. However, recent studies using molecular techniques suggest bacterial communities in the placenta, amniotic fluid, and meconium from healthy pregnancies. These findings have led many scientists to challenge the “sterile womb paradigm” and propose that microbiome acquisition instead begins in utero, an idea that would fundamentally change our understanding of gut microbiota acquisition and its role in human development. In this review, we provide a critical assessment of the evidence supporting these two opposing hypotheses, specifically as it relates to (i) anatomical, immunological, and physiological characteristics of the placenta and fetus; (ii) the research methods currently used to study microbial populations in the intrauterine environment; (iii) the fecal microbiome during the first days of life; and (iv) the generation of axenic animals and humans. Based on this analysis, we argue that the evidence in support of the “in utero colonization hypothesis” is extremely weak as it is founded almost entirely on studies that (i) used molecular approaches with an insufficient detection limit to study “low-biomass” microbial populations, (ii) lacked appropriate controls for contamination, and (iii) failed to provide evidence of bacterial viability. Most importantly, the ability to reliably derive axenic animals via cesarean sections strongly supports sterility of the fetal environment in mammals. We conclude that current scientific evidence does not support the existence of microbiomes within the healthy fetal milieu, which has implications for the development of clinical practices that prevent microbiome perturbations after birth and the establishment of future research priorities.

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“…The antibacterial activities of isolates were determined as described previously [10] against Salmonella typhimurium RSSK 95091, Escherichia coli BC 1402, Bacillus cereus BC 6830, Yersinia enterocolitica ATCC 27729, and Staphylococcus aureus ATCC 25923. For the determination of antifungal activities of the isolates, a previously described methodology was used [7] and antifungal activities were determined against Aspergillus niger, Penicillium chrysogenum, and Penicillium crustosum, respectively.…”

Section: Determination Of Antibacterial and Antifungal Activities Of mentioning

confidence: 99%

“…[7] Survival of the isolates under simulated in vitro digestion For the determination of survival ability of the isolates under mimicking gastrointestinal conditions, a previously described methodology was used. [10] Briefly, strains were grown in MRS media overnight and harvested by centrifugation (4000 g for 10 min at 4°C). The harvested cells were then resuspended in PBS to obtain an OD 600 of~1.0 and cell counts were determined.…”

Section: Determination Of Antibacterial and Antifungal Activities Of mentioning

confidence: 99%

Isolation and characterisation of lactic acid bacteria from traditional koumiss and kurut

İspirli

Dertli

2017

International Journal of Food Properties

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Lactic acid bacteria (LAB) were isolated from traditional koumiss and kurut samples and identification of these isolates revealed the presence of Lactobacillus fermentum, Enterococcus faecalis, Enterococcus durans, and Lactobacillus helveticus species. L. fermentum and L. helveticus strains were further tested for different characteristics. All tested strains produced heteropolymeric exopolysaccharides (EPS) with the presence of glucose and galactose as sugar monomers whereas three strains produced fructan type EPS as homopolymeric EPS. Important levels of antibacterial and antifungal activities were observed in these strains. All tested strains were sensitive to the antibiotics tested except streptomycin. Half of the tested strains showed important levels of survival ability under mimicking gastrointestinal conditions.

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Characterization of functional properties of Enterococcus faecium strains isolated from human gut

Cited by 38 publications

References 42 publications

Diversity and functional characteristics of lactic acid bacteria from traditional kefir grains

Diversity and functional characteristics of lactic acid bacteria from traditional kefir grains

A critical assessment of the “sterile womb” and “in utero colonization” hypotheses: implications for research on the pioneer infant microbiome

Isolation and characterisation of lactic acid bacteria from traditional koumiss and kurut

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