Characterization of Fusarium root rot disease in grafted watermelon

Zhang, Man; Yang, Xingping; Xu, Jianfei; Liu, Guang; Yao, Xiefeng; Ren, Runsheng

doi:10.1007/s10658-020-02013-w

Cited by 8 publications

(4 citation statements)

References 31 publications

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“…According to the results, both fungal species can be regarded as causal agents of fruit rot disease in watermelons. Our results are supported by previous studies that reported that Fusarium species are the cause of various disease symptoms in watermelons in tropical and subtropical regions around the world [ 73 , 74 , 75 , 76 ]. Prior to this study, F. solani , F. oxysporum , F. verticillioides , and F. chlamydosporum were considered to be the causal agents of fruit rot in watermelons in Nigeria [ 16 , 24 , 77 ].…”

Section: Discussionsupporting

confidence: 92%

“…niveum have been observed to cause Fusarium wilt disease in fruits grown in Korea [ 76 ] and Malaysia [ 74 ], respectively. On the other hand, Fusarium brachygibbosum and F. oxysporum have been shown to lead to vine decline symptoms in the United States [ 73 ] and root rot in China [ 75 ], respectively. Furthermore, other fungal species from the genera Alternaria , Aspergillus , Curvularia , Fusarium , Macrophomina , Phytophthora , Lasiodiplodia , Sclerotium , and Pythium have also been associated with fruit rot in watermelons.…”

Section: Discussionmentioning

confidence: 99%

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Identification and Characterization of Fusarium Species Causing Watermelon Fruit Rot in Northern Thailand

Nuangmek

Kumla

Khuna

et al. 2023

Plants

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Fruit rot caused by phytopathogenic fungi is one of the major diseases affecting watermelons (Citrullus lanatus) around the world, which can result in unmarketable fruits and significant economic losses. Fruit rot was observed on watermelons throughout the postharvest storage periods in Phayao Province, northern Thailand in 2022. For the present study, a total of ten fungal isolates were isolated from the rot lesions of watermelons. All obtained fungal isolates were then characterized in terms of their pathogenicity. The results indicated that only four fungal isolates caused rot disease with similar symptoms during the postharvest storage period. Based on their morphological characteristics, these four fungal isolates were identified as belonging to the genus Fusarium. Using multi-gene phylogenetic analyses with a combination of the translation elongation factor 1-alpha (tef-1), calmodulin (cam), and RNA polymerase second largest subunit (rpb2) genes, the fungal isolates were subsequently identified as Fusarium compactum and F. paranaense. Taken together, the results of this study indicate that F. compactum and F. paranaense cause fruit rot disease in watermelons. To the best of our knowledge, this is the first study to report F. compactum and F. paranaense as novel pathogens of watermelon fruit rot both in Thailand and elsewhere in the world.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Fusarium Species Causing Watermelon Fruit Rot in Northern Thailand

Nuangmek

Kumla

Khuna

et al. 2023

Plants

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“…In the present study, we identified eight Fusarium species based on morphological characteristics and phylogenetic analysis ( EF1-α ), including F. verticillioides , F. proliferatum, F. fujikuroi , F. asiaticum , F. equiseti, F. meridionale, F. graminearum and F. oxysporum , with observed isolation frequencies of 30.00, 22.67, 15.33, 7.33, 6.00, 5.33, 3.33 and 1.33%, respectively. Many studies have shown that Fusarium species are consistently isolated and identified in mixed infections with other Fusarium species or fungi on many crops in the field 21 , 22 . In the present study, F. verticillioides and F. proliferatum were consistently observed in mixed infections in CFSR on maize, with a mixed infection rate of 14.67%.…”

Section: Discussionmentioning

confidence: 99%

Symptoms and pathogens diversity of Corn Fusarium sheath rot in Sichuan Province, China

Wang

Sun

et al. 2021

Sci Rep

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To elucidate the symptoms and pathogens diversity of corn Fusarium sheath rot (CFSR), diseased samples were collected from 21 county-level regions in 12 prefecture-level districts of Sichuan Province from 2015 to 2018 in the present study. In the field, two symptom types appeared including small black spots with a linear distribution and wet blotches with a tawny or brown color. One hundred thirty-seven Fusarium isolates were identified based on morphological characteristics and phylogenetic analysis (EF1-α), and Koch’s postulates were also assessed. The results identified the isolates as 8 species in the Fusarium genus, including F. verticillioides, F. proliferatum, F. fujikuroi, F. asiaticum, F. equiseti, F. meridionale, F. graminearum and F. oxysporum, with isolation frequencies of 30.00, 22.67, 15.33, 7.33, 6.00, 5.33, 3.33 and 1.33%, respectively. Fusariumverticillioides and F. proliferatum were the dominant and subdominant species, respectively. Two or more Fusarium species such as F. verticillioides and F. proliferatum were simultaneously identified at a mixed infection rate of 14.67% in the present study. The pathogenicity test results showed that F. proliferatum and F. fujikuroi exhibited the highest virulence, with average disease indices of 30.28 ± 2.87 and 28.06 ± 1.96, followed by F. equiseti and F. verticillioides, with disease indices of 21.48 ± 2.14 and 16.21 ± 1.84, respectively. Fusarium asiaticum, F. graminearum and F. meridonale showed lower virulence, with disease indices of 13.80 ± 2.07, 11.57 ± 2.40 and 13.89 ± 2.49, respectively. Finally, F. orysporum presented the lowest virulence in CFSR, with a disease index of 10.14 ± 1.20. To the best of our knowledge, this is the first report of F. fujikuroi, F. meridionale and F. asiaticum as CFSR pathogens in China.

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“…Proteomic analysis had revealed that FON led to the significant changes in expression of abundant proteins, and the potential candidate proteins involved in stress or defense had great relevance to the biological pathways which linked to the watermelon-FON pathosystem ( Zhang et al, 2018 ). Besides, FON was identified as the most prevalent fungal strain which was isolated from the root of grafted watermelon infected by root rot and generated an increasing death rate of grafted watermelon seedlings ( Zhang et al, 2021a , b ). The sugar concentrations in root exudates of varied watermelon varieties could induce the diverse growth situation of watermelon by changing the growth of FON in rhizosphere ( Hao W. et al, 2011 ; Ma et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Variation of Soil Microbial Community and Sterilization to Fusarium oxysporum f. sp. niveum Play Roles in Slightly Acidic Electrolyzed Water-Alleviated Watermelon Continuous Cropping Obstacle

et al. 2022

Front. Microbiol.

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It is critical to exploit technologies for alleviating watermelon continuous cropping obstacle which frequently occurs and results in the limiting production and economic losses of watermelon. This study aimed to explore the effects of slightly acidic electrolyzed water (SAEW) on watermelon continuous cropping obstacles. The results showed that SAEW significantly improved the growth of watermelon seedlings cultivated in continuous cropping soil and caused a mass of changes to the diversity of the soil microbial community. Compared with Con, SAEW decreased the diversity index of bacteria by 2%, 0.48%, and 3.16%, while it increased the diversity index of fungus by 5.68%, 10.78%, and 7.54% in Shannon, Chao1, and ACE index, respectively. Besides, the enrichment level of Fusarium oxysporum f. sp. niveum (FON) was remarkably downregulated by 50.2% at 14 days of SAEW treatment, which could decrease the incidence of Fusarium wilt disease. The wet and dry weights of FON mycelia in the fluid medium were depressed more than 93%, and the number of FON colonies in continuous cropping soil was reduced by 83.56% with SAEW treatment. Additionally, a strong correlation between watermelon, FON, and SAEW was presented by correlation analysis. Furthermore, the content of endogenous reactive oxygen species (ROS) was over quadruply increased by SAEW, which may contribute to the sterilizing effect of SAEW on FON. Taken together, our findings demonstrated that exogenous SAEW could alter the soil microbial diversity and decrease the accumulation of FON, which improved the growth of watermelon seedlings and finally alleviated continuous cropping obstacles of watermelon.

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Characterization of Fusarium root rot disease in grafted watermelon

Cited by 8 publications

References 31 publications

Identification and Characterization of Fusarium Species Causing Watermelon Fruit Rot in Northern Thailand

Identification and Characterization of Fusarium Species Causing Watermelon Fruit Rot in Northern Thailand

Symptoms and pathogens diversity of Corn Fusarium sheath rot in Sichuan Province, China

Variation of Soil Microbial Community and Sterilization to Fusarium oxysporum f. sp. niveum Play Roles in Slightly Acidic Electrolyzed Water-Alleviated Watermelon Continuous Cropping Obstacle

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