Characterization of galectin-1 from Chinese giant salamanders Andrias davidianus and its involvements during immune response

Yang, Hui; Lan, Qingjing; Liu, Ranran; Cui, Dan; Liu, Haixia; Xiong, Dongmei; Li, Fenggang; Liu, Xiaolin; Wang, Lixin

doi:10.1016/j.dci.2017.01.004

Cited by 16 publications

(14 citation statements)

References 58 publications

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“…; Yang et al . ). However, traditions aside, the A. davidianus blood is a wasteful by‐product of the A. davidianus meat production industry; hence, it is poured into the local environment.…”

Section: Introductionmentioning

confidence: 97%

Purification, characterization and application of a novel antimicrobial peptide from Andrias davidianus blood

Pei

Feng

Ren

et al. 2017

Lett Appl Microbiol

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Significance and Impact of the Study: This is the pioneer study on screening and isolation of antimicrobial peptide from the blood of Andrias davidianus. Here, we have developed a novel method by combining magnetic liposomes adsorption with reversed-phase high-performance liquid chromatography to purify and screen the antimicrobial peptides. From this screen, we identified a novel antimicrobial peptide which we name as andricin B. Andricin B is unique as it checks the growth of both Gram-positive and Gram-negative bacteria as well as few fungal species. AbstractThe Andrias davidianus has been known as a traditional Chinese medicine for a long time. Its blood is considered as a waste or by-product of the meat production industry. Although there are reports on isolation of the antimicrobial peptides from different resources, there are no reports of their isolation from A. davidianus blood. In this work, an antimicrobial peptide, andricin B, was isolated from the blood of A. davidianus by an innovative method in which the magnetic liposome adsorption was combined with reversed-phase high-performance liquid chromatography. The structure, antimicrobial activity and safety of andricin B were further investigated. Amino acid sequence was determined by N-terminal sequencing and found to be Gly-Leu-Thr-Arg-Leu-Phe-Ser-Val-Ile-Lys. Circular dichroism (CD) spectra and prediction of three-dimensional structure by bioinformatics software suggested the presence of a well-defined random coil conformation. Andricin B was found to be active against all bacteria tested in this study as well as some fungi. The minimum inhibitory concentrations (MICs) were in the range 8-64 lg ml À1 . Moreover, the haemolytic testing also suggested that andricin B could be considered safe at the MICs. Finally, andricin B was shown to inhibit the growth of Staphylococcus aureus in the cooked meat of A. davidianus. This study shows that andricin B is a promising novel antimicrobial peptide that may provide further insights towards the development of new drugs.

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Section: Introductionmentioning

confidence: 97%

Purification, characterization and application of a novel antimicrobial peptide from Andrias davidianus blood

Pei

Feng

Ren

et al. 2017

Lett Appl Microbiol

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“…Andrias davidianus is widely recognized in traditional medicine as a cure‐all against many illnesses from colds to arthritis and has been even described as anticancer (Cunningham et al ; Qi et al ; Yang et al ). Despite this, the AMPs in these ancient balms have not been well characterized.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover artificially cultured A. davidianus is a favourite food for local people. Eating A. davidianus is believed to improve health and longevity according to Chinese folklore (Todorov et al ; Qi et al ; Yang et al ). The blood of A. davidianus is considered a byproduct of the food processing industry and is usually disposed of in the environment as waste.…”

Section: Introductionmentioning

confidence: 99%

Structure and mode of action of a novel antibacterial peptide from the blood of Andrias davidianus

Pei

Chen

Jin

et al. 2019

Letters in Applied Microbiology

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Significance and Impact of the Study: The results of this study suggested the new perspectives about the mode of action of antimicrobial peptide (AMP) active against sensitive bacteria. The AMP was able to be in a random coiled state in aqueous solution but to change to a more rigid one in the presence of sensitive bacteria. Exposure to AMP might not lead to immediate death of treated bacteria, rather bacteria concentration decreased gradually flattening at a sublethal stage. These findings will help people to understand better how the AMPs activate against sensitive bacteria. AbstractAndrias davidianus is widely recognized in traditional medicine as a cure-all to treat a plethora of ailments. In a previous study, a novel antibacterial peptide named andricin B was isolated from A. davidianus blood. In this study, we investigated andricin B structure and its mode of action. Circular dichroism spectra suggested that andricin B adopts a random coil state in aqueous solution and a more rigid conformation in the presence of bacteria. Moreover propidium iodide/fluorescein diacetate double staining indicated that bacteria treated with andricin B were not immediately eliminated. Rather, there is a gradual bacterial death, followed by a sublethal stage. Scanning electronic microscope imaging indicates that andricin B might form pores on cell membranes, leading to the release of cytoplasmic contents. These results were consistent with flow cytometry analysis. Furthermore, Fourier transform infrared spectroscopy suggests that andricin B induces changes in the chemical properties in the areas surrounding these "pores" on the cell membranes.

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“…CLRs, the other transmembrane PRRs, are able to recognize carbohydrate structures of bacteria, fungi, parasites, and virus through a unique structure known as "C-type carbohydrate recognition domain (CRD)'' or ''C-type lectin domain" (65,66). The CLR pathway initiates innate immune responses, including phagocytosis, activation of the complement system, and enhanced NK cell activity, and regulates adaptive immune responses (20,65).…”

Section: Innate Immune Systemmentioning

confidence: 99%

The Immune System and the Antiviral Responses in Chinese Giant Salamander, Andrias davidianus

et al. 2021

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The Chinese giant salamander, belonging to an ancient amphibian lineage, is the largest amphibian existing in the world, and is also an important animal for artificial cultivation in China. However, some aspects of the innate and adaptive immune system of the Chinese giant salamander are still unknown. The Chinese giant salamander iridovirus (GSIV), a member of the Ranavirus genus (family Iridoviridae), is a prominent pathogen causing high mortality and severe economic losses in Chinese giant salamander aquaculture. As a serious threat to amphibians worldwide, the etiology of ranaviruses has been mainly studied in model organisms, such as the Ambystoma tigrinum and Xenopus. Nevertheless, the immunity to ranavirus in Chinese giant salamander is distinct from other amphibians and less known. We review the unique immune system and antiviral responses of the Chinese giant salamander, in order to establish effective management of virus disease in Chinese giant salamander artificial cultivation.

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Characterization of galectin-1 from Chinese giant salamanders Andrias davidianus and its involvements during immune response

Cited by 16 publications

References 58 publications

Purification, characterization and application of a novel antimicrobial peptide from Andrias davidianus blood

Purification, characterization and application of a novel antimicrobial peptide from Andrias davidianus blood

Structure and mode of action of a novel antibacterial peptide from the blood of Andrias davidianus

The Immune System and the Antiviral Responses in Chinese Giant Salamander, Andrias davidianus

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