Characterization of genetic determinants involved in antibiotic resistance in Aeromonas spp. and fecal coliforms isolated from different aquatic environments

Carnelli, Alessandro; Mauri, Federica; Demarta, Antonella

doi:10.1016/j.resmic.2017.02.006

Cited by 31 publications

(21 citation statements)

References 35 publications

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“…2000; Janda and Abbott 2010; Carnelli et al . 2017). Our results have similarities with published data in view of the susceptibility values of isolates when evaluated using CLSI and EUCAST values.…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, Schmidt et al (2001) reported that 69% of the Aeromonas isolates from fish were oxytetracyclineresistant and possessed the genes tetA and tetE, while 48% displayed multi-resistance. Several researchers have reported that tetracycline resistance genes can be transferred horizontally by R-plasmids (Schmidt et al 2001;Carnelli et al 2017;Capkin et al 2017). This explains how WT isolates carry tetracycline resistance genes.…”

Section: Tetracyclinementioning

confidence: 99%

See 1 more Smart Citation

The determination of antimicrobial susceptibility by MIC and epidemiological cut‐off values and the detection of resistance genes in Aeromonas species isolated from cultured fish

Duman

Satıcıoğlu

Altun

2020

Lett. Appl. Microbiol.

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Significance and Impact of the Study: In this study, we investigated phenotypic and genotypic antimicrobial resistance characteristics by a novel method based on epidemiological cutoff values. This is the second comprehensive study on the antimicrobial susceptibility characteristics of Aeromonas species using NRI and epidemiological cutoff values. The present research is related to our previous researches focussed on the identification of motile Aeromonads, their prevalence in relation to different fish lengths, seasons and regions, and covered the investigation of Lactococcus garvieae, Yersinia ruckeri, Flavobacterium spp., Enterobacter spp. and Citrobacter spp.

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Section: Resultsmentioning

confidence: 99%

Section: Tetracyclinementioning

confidence: 99%

The determination of antimicrobial susceptibility by MIC and epidemiological cut‐off values and the detection of resistance genes in Aeromonas species isolated from cultured fish

Duman

Satıcıoğlu

Altun

2020

Lett. Appl. Microbiol.

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“…Further, Serratia, Aeromonas , and Pantoea were identified as apparent on meat. All these bacteria are widely distributed in environmental and pork samples (Jiang and Shi, 2013; Greig et al, 2015; Roberts and Schwarz, 2016; Møretrø and Langsrud, 2017), and various ARGs have been detected in antibiotics resistant strains belonging to these bacterial genera (Batah et al, 2015; Liu et al, 2015; Le et al, 2016; Carnelli et al, 2017). Contrary, over 40% of the bands from the soil and fecal sample gels were identified as uncultured bacteria (Supplementary Table S4), and accounted for the vast majority of the total bacteria as expected from various environmental samples (Rappé and Giovannoni, 2003).…”

Section: Discussionmentioning

confidence: 99%

From Pig Breeding Environment to Subsequently Produced Pork: Comparative Analysis of Antibiotic Resistance Genes and Bacterial Community Composition

et al. 2019

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It is well verified that pig farms are an important reservoir and supplier of antibiotic resistance genes (ARGs). However, little is known about the transmission of ARGs between the breeding environment and subsequently produced pork. This study was conducted to investigate if ARGs and associated host bacteria spread from the breeding environment onto the meat through the food production chain. We thus analyzed the occurrence and abundance of ARGs, as well as comparing both ARG and bacterial community compositions in farm soil, pig feces and pork samples from a large-scale pig farm located in Xiamen, People’s Republic of China. Among the 26 target ARGs, genes conferring resistance to sulfonamide, trimethoprim, aminoglycoside, chloramphenicol, macrolide, florfenicol, and tetracycline were observed at high frequency in both the pig breeding environment and pork. The prevalence of ARGs in pork was surprisingly consistent with breeding environments, especially between the pork and feces. The relative abundance of 10 representative ARGs conferring resistance to six classes of antibiotics ranged from 3.01 × 10-1 to 1.55 × 10-6 copies/16S rRNA copies. The ARGs conferring resistance to sulfanilamide (sulI and sulII), aminoglycoside (aadA), and tetracycline [tet(A) and tet(M)] were most highly abundant across most samples. Samples from feces and meat possessed a higher similarity in ARG compositions than samples from the farms soil. Enterobacteriaceae found on the meat samples were further identical with previously isolated multidrug-resistant bacteria from the same pig farm. Our results strongly indicate that ARGs can be potentially spreading from pig breeding environment to meat via the pork industry chain, such as feed supply, pig feeding and pork production.

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“…Microorganisms harboring resistance genes end up in water [2] and soil [3]. Wastewater [4], agricultural runoff [5], and hospital waste [6] have been reported as sources of antibiotic resistance in the aquatic environment. Water contaminated with antibiotic-resistant bacteria (ARB) has the potential to affect aquatic biodiversity [7,8] and human health adversely.…”

Section: Introductionmentioning

confidence: 99%

Presence of Antibiotic-Resistant Escherichia coli in Wastewater Treatment Plant Effluents Utilized as Water Reuse for Irrigation

Aslan

Cole

Bhattacharya

et al. 2018

Water

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Providing safe water through water reuse is becoming a global necessity. One concern with water reuse is the introduction of unregulated contaminants to the environment that cannot be easily removed by conventional wastewater treatment plants (WWTP). The occurrence of ampicillin, sulfamethoxazole, ciprofloxacin, and tetracycline-resistant Escherichia coli through the treatment stages of a WWTP (raw sewage, post-secondary, post-UV and post-chlorination) was investigated from January to May 2016. The highest concentrations of antibiotic resistant E. coli in the effluent were detected in April after rainfall. Ampicillin-resistant E. coli was the most common at the post UV and chlorination stages comprising 63% of the total E. coli population. The minimum inhibitory concentration (MIC) analysis showed that one in five isolates was resistant to three or more antibiotics, and the majority of these E. coli were resistant to ampicillin, followed by sulfamethoxazole and ciprofloxacin. The highest MIC was detected at the finished water after application of multiple disinfection methods. Tetracycline resistance was the least observed among others, indicating that certain drug families may respond to wastewater treatment differently. Currently, there are no policies to enforce the monitoring of antibiotic-resistant pathogen removal in WWTP. Better guidelines are needed to better regulate reuse water and prevent health risk upon exposure to antibiotic-resistant bacteria.

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Characterization of genetic determinants involved in antibiotic resistance in Aeromonas spp. and fecal coliforms isolated from different aquatic environments

Cited by 31 publications

References 35 publications

The determination of antimicrobial susceptibility by MIC and epidemiological cut‐off values and the detection of resistance genes in Aeromonas species isolated from cultured fish

The determination of antimicrobial susceptibility by MIC and epidemiological cut‐off values and the detection of resistance genes in Aeromonas species isolated from cultured fish

From Pig Breeding Environment to Subsequently Produced Pork: Comparative Analysis of Antibiotic Resistance Genes and Bacterial Community Composition

Presence of Antibiotic-Resistant Escherichia coli in Wastewater Treatment Plant Effluents Utilized as Water Reuse for Irrigation

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