Characterization of Glucosylceramides in thePolygonaceae,Rumex obtusifoliusL. Injurious Weed

Lipid profiles of detergent resistant fractions of the plasma membrane in oat and rye in association with cold acclimation and freezing tolerance

“…Sample fractions containing GlcCer was transferred to a Sep-Pak Plus Silica Cartridge (Waters, Milford, MA, USA) coupled to a glass syringe barrel and chloroform/acetic acid (100:1, v/v) was passed through the column. The fractions containing GlcCer were then eluted with chloroform/methanol (2:1, v/v) and injected into a LC-10AT pump (Shimadzu Scientific) coupled with ACQUITY TQD tandem quadrupole mass spectrometer (Waters) as described in Watanabe et al [45]. Samples were separated on a 3 µm TSKgel ODS-100Z column (Tosoh, Tokyo, Japan) eluted with a gradient from 80% solvent A (methanol/formic acid, 1000/1, v/v)/20% solvent B (water/formic acid, 1000/1, v/v) to 100% solvent A for 30 min and then solvent A for 70 min at a flow rate of 200 µL/min.…”

“…The following conditions were used for detection: capillary voltage, 3 kV; desolvation gas flow, 600 L/h; nebulizer gas flow, 50 L/h; source temp., 120°C; and collision gas flow, 0.3 mL/min (4–5 mbar). Identification of various GlcCer molecular species was carried out based on 30 pairs of precursor ions [M + H] + and product ions of sphingoid base moieties as listed in Watanabe et al [45] in the positive ionization MRM mode.…”

Characterization of Glucosylceramides in Leaves of the Grass Family (Poaceae): Pooideae Has Unsaturated Hydroxy Fatty Acids

“…4) It can be assumed from the previous results 4,10,22,25) and present data that 2-hydroxy fatty acids having a C 24 chain length [i.e., 24h:0 and/or 24h:1] are normally present at a certain level in the leaves from any species of plant. It has been presumed that glucosylceramide molecular species containing 4-hydroxy-8-sphingenines [i.e., t18:1(8Z) and t18:1(8E)] would be of primary importance in establishing the possible thermal phase transition of membrane lipids.…”

“…4,6,9) 2-Hydroxy tetracosenic acid (24h:1) has been found in oat, wheat and rye, and it has been reported that such monoenoic 2-hydroxy fatty acids as 2-hydroxy docosenic acid (22h:1) and 24h:1 have only been found in several species of chilling-resistant plants. 4,10) While these fatty acids have not been detected in the glucosylceramides from rice and maize, it is still unknown whether the only glucosylceramides in oat, wheat and rye are monoenoic 2-hydroxy fatty acids among various groups of Poaceae. Several functions have been ascribed to glucosylceramides and their components, including plant-fungus interaction 11) and membrane stability to temperature and aluminum stresses.…”

Pharmacological screening of Polygonaceae species and isolation of biologically active compounds from Rumex aquaticus L. and Rumex thyrsiflorus Fingerh.

“…The observed high content of n-9 monoenoic 2-hydroxy fatty acids with 22 and 24 carbonchain lengths is unique. 59 The fatty acid profiles of 20 Spanish wild vegetables (among them R. pulcher and R. papillaris) were evaluated by GC with FID detection. It was observed that the samples in which the leaves predominated in their edible parts in general contained the highest amounts of polyunsaturated fatty acid, with R. pulcher outstanding as concerns its high polyunsaturated/saturated fatty acid ratio.…”