Lipid profiles of detergent resistant fractions of the plasma membrane in oat and rye in association with cold acclimation and freezing tolerance

Abstract

Cold acclimation (CA) results in alteration of the plasma membrane (PM) lipid composition in plants, which plays a crucial role in the acquisition of freezing tolerance via membrane stabilization. Recent studies have indicated that PM structure is consistent with the fluid mosaic model but is laterally non-homogenous and contains microdomains enriched in sterols, sphingolipids and specific proteins. In plant cells, the function of these microdomains in relation to CA and freezing tolerance is not yet fully understood. The present study aimed to investigate the lipid compositions of detergent resistant fractions of the PM (DRM) which are considered to represent microdomains. They were prepared from leaves of low-freezing tolerant oat and high-freezing tolerant rye. The DRMs contained higher proportions of sterols, sphingolipids and saturated phospholipids than the PM. In particular, one of the sterol lipid classes, acylated sterylglycoside, was the predominant sterol in oat DRM while rye DRM contained free sterol as the major sterol. Oat and rye showed different patterns (or changes) of sterols and 2-hydroxy fatty acids of sphingolipids of DRM lipids during CA. Taken together, these results suggest that CA-induced changes of lipid classes and molecular species in DRMs are associated with changes in the thermodynamic properties and physiological functions of microdomains during CA and hence, influence plant freezing tolerance.

Sphingolipids: towards an integrated view of metabolism during the plant stress response

“…Acclimation capacity was correlated with changes in the content of TAGs (triacylglycerols), MGDG (monogalactosyldiacylglycerol), DGDG (digalactosyldiacylglycerol) and a GlcCer (Degenkolbe et al, 2012). Analysis of oat, rye and Arabidopsis lipid profiles during cold acclimation demonstrated that GlcCer contents decreased in the plasma membrane, whereas they were unchanged in microdomains (Minami et al, 2009;Takahashi et al, 2016). These changes could contribute to a greater hydration of the plasma membrane that could, in turn, increase membrane stability during cold stress.…”

Large-Scale Phosphoproteomic Study of Arabidopsis Membrane Proteins Reveals Early Signaling Events in Response to Cold

“…Therefore, perception of and response to a drop in temperature is essential for plants that originated in tropic and sub-tropic regions to deploy survival mechanisms and for those in temperate climatic zones to start the acclimation processes and prepare for upcoming freezing stress. Responses to cold under acclimating and freezing conditions are studied extensively on both genomic and proteomic levels [ 7 , 8 , 9 , 10 , 11 , 12 , 13 ]. However, there is little or no information available on how plants sense temperature drops over a short time (i.e., seconds and minutes) and how they respond and form adaptive signals to the cold.…”

Lipid peroxidation of cell membranes in the formation and regulation of plant protective reactions

“…Обидва процеси врешті-решт призводять до пошкодження молекулярних клітин, що може сприяти розвитку різних патологічних станів та прискоренню процесів старіння (Ayala et al, 2014). ПОЛ є первинною реакцією рослинних клітин на дію стресів, що призводить до руйнування ліпідного комплексу мембран, порушуючи їхні транспортні функції, а також пригнічуючи процеси генерації енергії, що в кінцевому результаті знижує життєдіяльність клітин (Baraboi, 1991;Takahashi et al, 2016). Водночас ці процеси індукують стрес-захисні реакції, що мають важливе значення для відновлення та адаптації функціонуючих структур, внаслідок підвищення ефективності ферментативного та неферментативного антиоксидантного захисту та чіткого контролю вмісту активних кисневих радикалів, ліпідних пероксидів і каталізаторів пероксидазних реакцій (Zhu, 2016;Laxa et al, 2019).…”